-
- György Hutvágner
- Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA.
-
- Phillip D. Zamore
- Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA.
抄録
<jats:p> In animals, the double-stranded RNA-specific endonuclease Dicer produces two classes of functionally distinct, tiny RNAs: microRNAs (miRNAs) and small interfering RNAs (siRNAs). miRNAs regulate mRNA translation, whereas siRNAs direct RNA destruction via the RNA interference (RNAi) pathway. Here we show that, in human cell extracts, the miRNA <jats:italic>let</jats:italic> - <jats:italic>7</jats:italic> naturally enters the RNAi pathway, which suggests that only the degree of complementarity between a miRNA and its RNA target determines its function. Human <jats:italic>let</jats:italic> - <jats:italic>7</jats:italic> is a component of a previously identified, miRNA-containing ribonucleoprotein particle, which we show is an RNAi enzyme complex. Each <jats:italic>let</jats:italic> - <jats:italic>7</jats:italic> –containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells. </jats:p>
収録刊行物
-
- Science
-
Science 297 (5589), 2056-2060, 2002-09-20
American Association for the Advancement of Science (AAAS)
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1363951796191402752
-
- NII論文ID
- 80015560451
-
- ISSN
- 10959203
- 00368075
-
- データソース種別
-
- Crossref
- CiNii Articles