Thermal denaturation and autolysis profiles of myofibrillar proteins of mantle muscle of jumbo squid Docidicus gigas.

• KONNO KUNIHIKO

  Graduate School of Fisheries Sciences, Hokkaido University

• YOUNG-JE CHO

  Department of Food Science and Technology, Pukyong National University

• YOSHIOKA TAKEYA

  Graduate School of Fisheries Sciences, Hokkaido University Hokkaido Industrial Technology Center

• SHINHO PARK

  Graduate School of Fisheries Sciences, Hokkaido University

• SEKI NOBUO

  Graduate School of Fisheries Sciences, Hokkaido University

抄録

Jumbo squid was very similar to Japanese common squid in terms of myofibrillar Ca²⁺-, Mg²⁺- and K⁺(EDTA)-ATPase activities. Myofibrils of jumbo squid were significantly stabilized upon addition of Ca²⁺ and destabilized by increasing KCl concentration for heating. Incubation of muscle homogenate of jumbo squid induced a selective cleavage of myosin into two major fragments and the cleavage was inhibited by EDTA. Autolysis was prominent at and above 0.3 M NaCl where myosin filaments dissolve. The enzyme involved in the autolysis was proved to be unstable showing maximal autolysis rate at 25°C. Washing the homogenate partially reduced the autolysis activity.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 69 (1), 204-209, 2003

  公益社団法人 日本水産学会

キーワード

• ATPase activity
• autolysis
• calcium ion
• inactivation
• jumbo squid
• thermal myofibrils

詳細情報

• CRID: 1390282679406425856
• NII論文ID: 130003904019
• NII論文ID: 80015836806
• NII書誌ID: AA10993718
• DOI: 10.1046/j.1444-2906.2003.00607.x
• COI: 1:CAS:528:DC%2BD3sXhvFemurk%3D
• ISSN: 14442906
• ISSN: 09199268
• NDL書誌ID: 6450845
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6450845
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可