hycu-hr6, a Large homologous region of Hyphantria cunea nucleopolyhedrovirus, is dispensable for virus replication in culture cells

• Kamiya Katsumi

  Gifu Prefectural Institute of Bioindustrial Technology

• Okimoto Naoaki

  Laboratory of Biodynamics Graduate School of Bioagricultural Sciences, Nagoya University

• Ikeda Motoko

  Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University

• Kunimi Yasuhisa

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology

• Kobayashi Michihiro

  Laboratory of Biodynamics Graduate School of Bioagricultural Sciences, Nagoya University

• Kawamura Satoshi

  Gifu Prefectural Institute of Bioindustrial Technology

書誌事項

タイトル別名

• Genotypic Variation of a Wild Isolate of <i>Hyphantria cunea</i> Nucleopolyhedrovirus
• Genotypic variation of a wild isolate of Hyphantria cunea nucleopolyhedrovirus

抄録

Thirty clones were derived by plaque assay on FRI-Splm 1229 cells from a wild isolate of Hyphantria cunea nucleopolyhedrovirus (HycuNPV). Analysis with restriction endonucleases (RENs) revealed that 29 clones out of 30 were distinct in their REN patterns, suggesting a very high degree of naturally occurring genotypic variation. Budded virion (BV) yields of 10 clones were examined in FRI-Splm 1229 cells at 48 and 72 h postinfection (pi), showing that productivity of BV and polyhedrin in the cell culture was different among clones. Two clones with higher BV productivity and two clones with lower BV productivity were each selected and examined for the virulence in per os inoculated H. cunea larvae as well as for the production of BV and polyhedrin in the cell culture. The virulence was different among selected clones, although no clear correlation was made between the virulence for H. cunea larvae and the productivity of BVs and/or polyhedrin in the cell culture. These results indicate that the wild isolate of HycuNPV consists of diverged variants that are distinct in genotype and biological activity.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｓｅｒｉｃｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｓｅｒｉｃｏｌｏｇｙ 72 (1), 57-64, 2003

  社団法人 日本蚕糸学会

キーワード

• <i>Hyphantria cunea</i> nucleopolyhedrovirus
• HycuNPV
• genotypic variant
• restriction endonuclease analysis
• virulence
• budded virus
• polyhedrin

詳細情報

• CRID: 1390282680172962048
• NII論文ID: 10019291376
• NII論文ID: 130004464110
• NII論文ID: 80015845985
• NII書誌ID: AA11558849
• DOI: 10.11416/jibs.72.57
• DOI: 10.11416/jibs.75.57
• ISSN: 18847978
• ISSN: 13468073
• NDL書誌ID: 8019758
• NDL書誌ID: 6485292
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8019758
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6485292
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可