Overexpression of <i>SOD2</i> Increases Salt Tolerance of Arabidopsis

DOI PDF 4 Citations
  • Xiuhua Gao
    Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250014, China
  • Zhonghai Ren
    Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250014, China
  • Yanxiu Zhao
    Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250014, China
  • Hui Zhang
    Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250014, China

Abstract

<jats:title>Abstract</jats:title> <jats:p>The yeast (Schizosaccharomyces pombe) SOD2 (Sodium2) gene was introduced into Arabidopsis under the control of the cauliflower mosaic virus 35S promoter. Transformants were selected for their ability to grow on medium containing kanamycin. Southern- and northern-blot analyses confirmed that SOD2 was transferred into the Arabidopsis genome. There were no obvious morphological or developmental differences between the transgenic and wild-type (wt) plants. Several transgenic homozygous lines and wt plants (control) were evaluated for salt tolerance and gene expression. Overexpression of SOD2 in Arabidopsis improved seed germination and seedling salt tolerance. Analysis of Na+ and K+ contents of the symplast and apoplast in the parenchyma cells of the root cortex and mesophyll cells in the spongy tissue of the leaf showed that transgenic lines accumulated less Na+ and more K+ in the symplast than the wt plants did. The photosynthetic rate and the fresh weight of the transgenic lines were distinctly higher than that of wt plants after NaCl treatment. Results from different tests indicated that the expression of the SOD2 gene promoted a higher level of salt tolerance in vivo in transgenic Arabidopsis plants.</jats:p>

Journal

  • Plant Physiology

    Plant Physiology 133 (4), 1873-1881, 2003-12-01

    Oxford University Press (OUP)

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