Variation in lipid A structure in the pathogenic yersiniae

• Roberto Rebeil

• Robert K. Ernst

• Brian B. Gowen

• Samuel I. Miller

• B. Joseph Hinnebusch

抄録

Important pathogens in the genus Yersinia include the plague bacillus Yersinia pestis and two enteropathogenic species, Yersinia pseudotuberculosis and Yersinia enterocolitica . A shift in growth temperature induced changes in the number and type of acyl groups on the lipid A of all three species. After growth at 37°C, Y. pestis lipopolysaccharide (LPS) contained the tetra‐acylated lipid IV A and smaller amounts of lipid IV A modified with C10 or C12 acyl groups, Y. pseudotuberculosis contained the same forms as part of a more heterogeneous population in which lipid IV A modified with C16:0 predominated, and Y. enterocolitica produced a unique tetra‐acylated lipid A. When grown at 21°C, however, the three yersiniae synthesized LPS containing predominantly hexa‐acylated lipid A. This more complex lipid A stimulated human monocytes to secrete tumour necrosis factor‐α, whereas the lipid A synthesized by the three species at 37°C did not. The Y. pestis phoP gene was required for aminoarabinose modification of lipid A, but not for the temperature‐dependent acylation changes. The results suggest that the production of a less immunostimulatory form of LPS upon entry into the mammalian host is a conserved pathogenesis mechanism in the genus Yersinia , and that species‐specific lipid A forms may be important for life cycle and pathogenicity differences.

収録刊行物

• Molecular Microbiology

  Molecular Microbiology 52 (5), 1363-1373, 2004-04-28

  Wiley

キーワード

• Molecular Biology
• Microbiology

詳細情報

• CRID: 1363951794594027904
• NII論文ID: 80016726843
• DOI: 10.1111/j.1365-2958.2004.04059.x
• ISSN: 13652958
• ISSN: 0950382X
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2958.2004.04059.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2958.2004.04059.x
• データソース種別:
  • Crossref
  • CiNii Articles