Cell cycle-dependent Ca2+ oscillations in mouse embryos are regulated by nuclear targeting of PLCζ

DOI PDF 被引用文献11件
  • Mark G. Larman
    Department of Anatomy and Developmental Biology, University College London, London, WC1E 6BT, UK
  • Christopher M. Saunders
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff, CF14 4XN, UK
  • John Carroll
    Department of Physiology, University College London, London, WC1E 6BT, UK
  • F. Anthony Lai
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff, CF14 4XN, UK
  • Karl Swann
    Department of Anatomy and Developmental Biology, University College London, London, WC1E 6BT, UK

抄録

<jats:p>During the first cell cycle Ca2+ oscillations are regulated in a cell cycle-dependent manner, such that the oscillations are unique to M phase. How the Ca2+ oscillations are regulated with such cell cycle stage-dependency is unknown, despite their importance for egg activation and embryo development. We recently identified a novel, sperm-specific phospholipase C (PLCzeta; PLCζ) that triggers Ca2+ oscillations similar to those caused by sperm. We show that PLCζ-induced Ca2+ oscillations also occur exclusively during M phase. The cell cycle-dependency can be explained by PLCζ's localisation to the pronuclei, which depends specifically upon a nuclear localisation signal sequence. Preventing pronuclear localisation of PLCζ by mutation of the nuclear localisation signal, or by inhibiting pronuclear formation/import, can prolong Ca2+ oscillations or allow them to occur during interphase. These data suggest a novel mechanism for regulating a PLC through nuclear sequestration and may explain the cell cycle-dependent regulation of Ca2+ oscillations following fertilisation.</jats:p>

収録刊行物

被引用文献 (11)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ