Nanos suppresses somatic cell fate in <i>Drosophila</i> germ line

• Yoshiki Hayashi

  Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Higashiyama, Myodaiji, Okazaki 444-8787, Japan; Department of Molecular Biomechanics, School of Life Science, Graduate University for Advanced Studies, 38 Nishigonaka, Myodaiji, Okazaki 444-8585, Japan; and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Honcho, Kawaguchi 332-0012, Japan

• Makoto Hayashi

  Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Higashiyama, Myodaiji, Okazaki 444-8787, Japan; Department of Molecular Biomechanics, School of Life Science, Graduate University for Advanced Studies, 38 Nishigonaka, Myodaiji, Okazaki 444-8585, Japan; and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Honcho, Kawaguchi 332-0012, Japan

• Satoru Kobayashi

  Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Higashiyama, Myodaiji, Okazaki 444-8787, Japan; Department of Molecular Biomechanics, School of Life Science, Graduate University for Advanced Studies, 38 Nishigonaka, Myodaiji, Okazaki 444-8585, Japan; and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Honcho, Kawaguchi 332-0012, Japan

抄録

Nanos (Nos) is one of the evolutionarily conserved proteins known to direct germ-line development. In Drosophila , maternal Nos protein maintains transcriptional quiescence in the germ-line progenitors or pole cells to repress ectopic expression of somatic genes. Here we show that maternal Nos is required to establish and maintain germ-line identity by preventing apoptosis and somatic cell fate. The pole cells lacking maternal Nos were degraded by apoptosis during mid to late embryogenesis. When apoptosis was suppressed by Df(3L)H99 , some pole cells lacking Nos adopted somatic cell fates. These pole cells expressed somatic markers ectopically and lost the germ-line marker Vasa. We further found that some Nos-negative pole cells were able to migrate into the gonads, but they failed to develop as functional germ cells during postembryonic stages. We propose a model in which Nos establishes germ-line/soma dichotomy and is also required to maintain germ-line fate.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 101 (28), 10338-10342, 2004-07-06

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360292619098318592
• NII論文ID: 80016817966
• DOI: 10.1073/pnas.0401647101
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.0401647101
• データソース種別:
  • Crossref
  • CiNii Articles