The SapB morphogen is a lantibiotic-like peptide derived from the product of the developmental gene <i>ramS</i> in <i>Streptomyces coelicolor</i>

DOI Web Site 被引用文献12件
  • Shinya Kodani
    Department of Biology, Hofstra University, Hempstead, NY 11549; Department of Biochemistry, Health Sciences Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5; and Computational Biology Group and Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom
  • Michael E. Hudson
    Department of Biology, Hofstra University, Hempstead, NY 11549; Department of Biochemistry, Health Sciences Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5; and Computational Biology Group and Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom
  • Marcus C. Durrant
    Department of Biology, Hofstra University, Hempstead, NY 11549; Department of Biochemistry, Health Sciences Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5; and Computational Biology Group and Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom
  • Mark J. Buttner
    Department of Biology, Hofstra University, Hempstead, NY 11549; Department of Biochemistry, Health Sciences Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5; and Computational Biology Group and Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom
  • Justin R. Nodwell
    Department of Biology, Hofstra University, Hempstead, NY 11549; Department of Biochemistry, Health Sciences Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5; and Computational Biology Group and Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom
  • Joanne M. Willey
    Department of Biology, Hofstra University, Hempstead, NY 11549; Department of Biochemistry, Health Sciences Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5; and Computational Biology Group and Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich, Norfolk NR4 7UH, United Kingdom

抄録

<jats:p> SapB is a morphogenetic peptide that is important for aerial mycelium formation by the filamentous bacterium <jats:italic>Streptomyces coelicolor</jats:italic> . Production of SapB commences during aerial mycelium formation and depends on most of the genes known to be required for the morphogenesis of aerial hyphae. Furthermore, the application of purified SapB to mutants blocked in morphogenesis restores their capacity to form aerial hyphae. Here, we present evidence that SapB is a lantibiotic-like peptide that is derived by posttranslational modification from the product of a gene ( <jats:italic>ramS</jats:italic> ) in the four-gene <jats:italic>ram</jats:italic> operon, which is under the control of the regulatory gene <jats:italic>ramR</jats:italic> . We show that the product of another gene in the operon ( <jats:italic>ramC</jats:italic> ) contains a region that is similar to enzymes involved in the biosynthesis of lantibiotics, suggesting that it might be involved in the posttranslational processing of RamS. We conclude that SapB is derived from RamS through proteolytic cleavage and the introduction of four dehydroalanine residues and two lanthionine bridges. We provide an example of a morphogenetic role for an antibiotic-like molecule. </jats:p>

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