Metal ion-mediated polymer superquenching for highly sensitive detection of kinase and phosphatase activities
-
- Frauke Rininsland
- QTL Biosystems, 2778 Agua Fria Street, Santa Fe, NM 87507
-
- Wensheng Xia
- QTL Biosystems, 2778 Agua Fria Street, Santa Fe, NM 87507
-
- Shannon Wittenburg
- QTL Biosystems, 2778 Agua Fria Street, Santa Fe, NM 87507
-
- Xiaobo Shi
- QTL Biosystems, 2778 Agua Fria Street, Santa Fe, NM 87507
-
- Casey Stankewicz
- QTL Biosystems, 2778 Agua Fria Street, Santa Fe, NM 87507
-
- Komandoor Achyuthan
- QTL Biosystems, 2778 Agua Fria Street, Santa Fe, NM 87507
-
- Duncan McBranch
- QTL Biosystems, 2778 Agua Fria Street, Santa Fe, NM 87507
-
- David Whitten
- QTL Biosystems, 2778 Agua Fria Street, Santa Fe, NM 87507
抄録
<jats:p>An assay technology for high-throughput screening of kinase and phosphatase activities is introduced. The format is based upon superquenching of fluorescent-conjugated polymers by dye-labeled kinase/phosphatase peptide substrates. The sensor platform is composed of highly fluorescent-conjugated polyelectrolytes colocated with the phosphate coordinating metal ion gallium on microspheres. Phosphorylated peptide substrates containing a quencher bind specifically to the metal ions by means of phosphate groups, resulting in quench of polymer fluorescence. The modulation of fluorescence signal is proportional to kinase or phosphatase activity and is monitored as a turn-off or turn-on signal, respectively. The assay is homogeneous and simple and can be run either as an endpoint measurement or in a kinetic mode. The assay meets the sensitivity required for high-throughput screening of kinase or phosphatase inhibitors and is a valuable tool for drug discovery. A modified version of the assay allows for the detection of protein phosphorylation.</jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 101 (43), 15295-15300, 2004-10-19
Proceedings of the National Academy of Sciences
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1360292619197909888
-
- NII論文ID
- 80017017400
-
- ISSN
- 10916490
- 00278424
-
- データソース種別
-
- Crossref
- CiNii Articles