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- Fumitaka Kawai
- Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan
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- Momoko Shoda
- Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan
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- Rie Harashima
- Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan
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- Yoshito Sadaie
- Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan
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- Hiroshi Hara
- Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan
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- Kouji Matsumoto
- Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan
抄録
<jats:title>ABSTRACT</jats:title> <jats:p> Recently, use of the cardiolipin (CL)-specific fluorescent dye 10- <jats:italic>N</jats:italic> -nonyl-acridine orange (NAO) revealed CL-rich domains in the <jats:italic>Escherichia coli</jats:italic> membrane (E. Mileykovskaya and W. Dowhan, J. Bacteriol. <jats:bold>182:</jats:bold> 1172-1175, 2000). Staining of <jats:italic>Bacillus subtilis</jats:italic> cells with NAO showed that there were green fluorescence domains in the septal regions and at the poles. These fluorescence domains were scarcely detectable in exponentially growing cells of the <jats:italic>clsA</jats:italic> -disrupted mutant lacking detectable CL. In sporulating cells with a wild-type lipid composition, fluorescence domains were observed in the polar septa and on the engulfment and forespore membranes. Both in the <jats:italic>clsA</jats:italic> -disrupted mutant and in a mutant with disruptions in all three of the paralogous genes ( <jats:italic>clsA</jats:italic> , <jats:italic>ywjE</jats:italic> , and <jats:italic>ywiE</jats:italic> ) for CL synthase, these domains did not vanish but appeared later, after sporulation initiation. A red shift in the fluorescence due to stacking of two dye molecules and the lipid composition suggested that a small amount of CL was present in sporulating cells of the mutants. Mass spectrometry analyses revealed the presence of CL in these mutant cells. At a later stage during sporulation of the mutants the frequency of heat-resistant cells that could form colonies after heat treatment was lower. The frequency of sporulation of these cells at 24 h after sporulation initiation was 30 to 50% of the frequency of the wild type. These results indicate that CL-rich domains are present in the polar septal membrane and in the engulfment and forespore membranes during the sporulation phase even in a <jats:italic>B. subtilis</jats:italic> mutant with disruptions in all three paralogous genes, as well as in the membranes of the medial septa and at the poles during the exponential growth phase of wild-type cells. The results further suggest that the CL-rich domains in the polar septal membrane and engulfment and forespore membranes are involved in sporulation. </jats:p>
収録刊行物
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- Journal of Bacteriology
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Journal of Bacteriology 186 (5), 1475-1483, 2004-03
American Society for Microbiology
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詳細情報 詳細情報について
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- CRID
- 1363670320324814720
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- NII論文ID
- 80017202823
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- ISSN
- 10985530
- 00219193
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