Cardiolipin Domains in <i>Bacillus subtilis</i> Marburg Membranes

• Fumitaka Kawai

  Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan

• Momoko Shoda

  Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan

• Rie Harashima

  Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan

• Yoshito Sadaie

  Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan

• Hiroshi Hara

  Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan

• Kouji Matsumoto

  Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-ohkubo, Sakura, Saitama, Saitama 338-8570, Japan

抄録

Recently, use of the cardiolipin (CL)-specific fluorescent dye 10- N -nonyl-acridine orange (NAO) revealed CL-rich domains in the Escherichia coli membrane (E. Mileykovskaya and W. Dowhan, J. Bacteriol. 182: 1172-1175, 2000). Staining of Bacillus subtilis cells with NAO showed that there were green fluorescence domains in the septal regions and at the poles. These fluorescence domains were scarcely detectable in exponentially growing cells of the clsA -disrupted mutant lacking detectable CL. In sporulating cells with a wild-type lipid composition, fluorescence domains were observed in the polar septa and on the engulfment and forespore membranes. Both in the clsA -disrupted mutant and in a mutant with disruptions in all three of the paralogous genes ( clsA , ywjE , and ywiE ) for CL synthase, these domains did not vanish but appeared later, after sporulation initiation. A red shift in the fluorescence due to stacking of two dye molecules and the lipid composition suggested that a small amount of CL was present in sporulating cells of the mutants. Mass spectrometry analyses revealed the presence of CL in these mutant cells. At a later stage during sporulation of the mutants the frequency of heat-resistant cells that could form colonies after heat treatment was lower. The frequency of sporulation of these cells at 24 h after sporulation initiation was 30 to 50% of the frequency of the wild type. These results indicate that CL-rich domains are present in the polar septal membrane and in the engulfment and forespore membranes during the sporulation phase even in a B. subtilis mutant with disruptions in all three paralogous genes, as well as in the membranes of the medial septa and at the poles during the exponential growth phase of wild-type cells. The results further suggest that the CL-rich domains in the polar septal membrane and engulfment and forespore membranes are involved in sporulation.

収録刊行物

• Journal of Bacteriology

  Journal of Bacteriology 186 (5), 1475-1483, 2004-03

  American Society for Microbiology

キーワード

• Molecular Biology
• Microbiology

詳細情報

• CRID: 1363670320324814720
• NII論文ID: 80017202823
• DOI: 10.1128/jb.186.5.1475-1483.2004
• ISSN: 10985530
• ISSN: 00219193
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JB.186.5.1475-1483.2004
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