Protection of hepatocytes from apoptosis by a novel substance from actinomycetes culture medium
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- LIU Yang
- Department of Biochemistry, School of Medicine, Akita University
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- TAKAHASHI Saori
- Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)
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- OGASAWARA Hironobu
- Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)
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- SEO Han Geuk
- Department of Pharmacology, College of Medicine, Gyeongsang National University
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- KAWAGOE Masami
- Department of Biochemistry, School of Medicine, Akita University
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- HIRASAWA Fujiko
- Department of Biochemistry, School of Medicine, Akita University
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- GUO Naxin
- Department of Biochemistry, School of Medicine, Akita University
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- UENO Yasuharu
- Department of Biochemistry, School of Medicine, Akita University
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- KAMEDA Takashi
- Department of Biochemistry, School of Medicine, Akita University
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- SUGIYAMA Toshihiro
- Department of Biochemistry, School of Medicine, Akita University
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抄録
A novel substance, #675, found from an Streptomyces sp. SM675 culture medium, dose-dependently stimulates the proliferation of human functional liver cell 4 (FLC4). When FLC4 cells were incubated under conditions without fetal bovine serum (FBS), typical features of apoptotic cell death such as shrinkage and nuclear condensation appeared; high molecular weight (HMW) DNA fragments were found; and caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were cleaved. When FLC4 cells were incubated with #675 and without FBS, the cells grew healthy, no HMW DNA fragments were found, and caspase-3 and PARP cleavage weakened, suggesting that #675 protects FLC4 cells from apoptosis induced by FBS-deprivation. The quantitative reverse-transcribed polymerase chain reaction did not show differences in PARP or Bcl-2 mRNA expression in FLC4 cells incubated with or without #675, indicating other genes may be involved in this anti-apoptosis effect. These results show that #675 enhances FLC4 proliferation via an apoptosis-inhibition pathway, implying potential pharmacological and clinical applications.
収録刊行物
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- Biomedical Research
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Biomedical Research 26 (1), 9-14, 2005
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