Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in <i>Synechococcus</i> and <i>Arabidopsis</i>

• Rungaroon Waditee

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• Md. Nazmul H. Bhuiyan

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• Vandna Rai

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• Kenji Aoki

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• Yoshito Tanaka

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• Takashi Hibino

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• Shigetoshi Suzuki

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• Jun Takano

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• André T. Jagendorf

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• Tetsuko Takabe

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

• Teruhiro Takabe

  Research Institute, Graduate School of Environmental and Human Sciences, and School of Agriculture, Meijo University, Nagoya 468-8502, Japan; Shimadzu Company, Nakagyou-ku, Kyoto 604-8511, Japan; Department of Plant Biology, Cornell University, Ithaca, NY 14853; and Graduate School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan

抄録

Betaine is an important osmoprotectant, synthesized by many plants in response to abiotic stresses. Almost all known biosynthetic pathways of betaine are two-step oxidations of choline. Recently, a biosynthetic pathway of betaine from glycine, catalyzed by two N -methyltransferase enzymes, was found. Here, the potential role of N -methyltransferase genes for betaine synthesis was examined in a freshwater cyanobacterium, Synechococcus sp. PCC 7942, and in Arabidopsis plants. It was found that the coexpression of N -methyltransferase genes in Synechococcus caused accumulation of a significant amount of betaine and conferred salt tolerance to a freshwater cyanobacterium sufficient for it to become capable of growth in seawater. Arabidopsis plants expressing N -methyltransferase genes also accumulated betaine to a high level in roots, stems, leaves, and flowers and improved seed yield under stress conditions. Betaine levels were higher than those produced by choline-oxidizing enzymes. These results demonstrate the usefulness of glycine N -methyltransferase genes for the improvement of abiotic stress tolerance in crop plants.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 102 (5), 1318-1323, 2005-01-21

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361981469737516416
• NII論文ID: 80017266325
• DOI: 10.1073/pnas.0409017102
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.0409017102
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