Genetically Engineered Wine Yeast Produces a High Concentration of <scp>l</scp> -Lactic Acid of Extremely High Optical Purity

DOI Web Site 被引用文献15件
  • Satoshi Saitoh
    Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co., Miyoshi-cho, Aichi 470-0201
  • Nobuhiro Ishida
    Biotechnology Laboratory, Toyota Central R&D Labs, Inc., Nagakute-cho, Aichi 480-1192
  • Toru Onishi
    Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co., Miyoshi-cho, Aichi 470-0201
  • Kenro Tokuhiro
    Biotechnology Laboratory, Toyota Central R&D Labs, Inc., Nagakute-cho, Aichi 480-1192
  • Eiji Nagamori
    Biotechnology Laboratory, Toyota Central R&D Labs, Inc., Nagakute-cho, Aichi 480-1192
  • Katsuhiko Kitamoto
    Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
  • Haruo Takahashi
    Biotechnology Laboratory, Toyota Central R&D Labs, Inc., Nagakute-cho, Aichi 480-1192

抄録

<jats:title>ABSTRACT</jats:title> <jats:p> For mass production of lactic acid, we newly constructed a transgenic wine yeast strain that included six copies of the bovine <jats:sc>l</jats:sc> -lactate dehydrogenase gene on the genome. On fermentation in inexpensive cane juice-based medium, <jats:sc>l</jats:sc> -lactate production of this recombinant reached 122 g/liter and the optical purity was 99.9% or higher. </jats:p>

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