Novel mycolic acid-containing bacteria in the family Segniliparaceae fam. nov., including the genus Segniliparus gen. nov., with descriptions of Segniliparus rotundus sp. nov. and Segniliparus rugosus sp. nov.

DOI Web Site 被引用文献2件
  • W. Ray Butler
    Division of Tuberculosis Elimination, National Center for HIV, STD and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA
  • Margaret M. Floyd
    Division of Tuberculosis Elimination, National Center for HIV, STD and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA
  • June M. Brown
    Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA
  • Sean R. Toney
    Division of Tuberculosis Elimination, National Center for HIV, STD and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA
  • Maryam I. Daneshvar
    Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA
  • Robert C. Cooksey
    Division of Tuberculosis Elimination, National Center for HIV, STD and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA
  • Janice Carr
    Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA
  • Arnold G. Steigerwalt
    Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA
  • Nadege Charles
    Division of Tuberculosis Elimination, National Center for HIV, STD and Tuberculosis Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA

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<jats:p>Four strains of novel, rapidly growing, acid–alcohol-fast-staining bacteria were characterized with a polyphasic approach. Isolates were received by the Centers for Disease Control and Prevention from domestic health department laboratories for reference testing as unidentifiable, clinical mycobacteria. Bacteria were rod-shaped and produced non-pigmented (white to beige), non-photochromogenic, smooth or wrinkled-rough colonies on Middlebrook 7H10 and 7H11 media at 33 °C. The smooth and wrinkled colony forms were representative of two species with 68·0 and 72·0 mol% DNA G+C content. The cell wall contained <jats:italic>meso</jats:italic>-diaminopimelic acid and mycolic acids. Species were characterized by cellular fatty acids of C10 : 0, C14 : 0, C16 : 1<jats:italic>ω</jats:italic>9<jats:italic>t</jats:italic>, C16 : 0, C18 : 1<jats:italic>ω</jats:italic>9<jats:italic>c</jats:italic> and 10-methyl C18 : 0 (tuberculostearic acid). HPLC analysis of mycolic acids produced a novel late-emerging, genus-specific mycolate pattern. TLC analysis demonstrated a novel <jats:italic>α</jats:italic> <jats:sup>+</jats:sup>-mycolate. Species were 98·9 % similar by comparison of 16S rRNA gene sequences; however, the DNA–DNA association was <28 %. Phylogenetic analysis of 16S rRNA gene sequences demonstrated an association with <jats:italic>Rhodococcus equi</jats:italic>, although a DNA–DNA relatedness value of 2 % did not support a close relationship. PCR analysis of a proposed, selected actinomycete-specific 439 bp fragment of the 65 kDa heat-shock protein was negative for three of the four isolates. The creation of <jats:italic>Segniliparaceae</jats:italic> fam. nov. is proposed to encompass the genus <jats:italic>Segniliparus</jats:italic> gen. nov., including two novel species, the type species <jats:italic>Segniliparus rotundus</jats:italic> sp. nov. and <jats:italic>Segniliparus rugosus</jats:italic> sp. nov., with the respective type strains CDC 1076<jats:sup>T</jats:sup> (=ATCC BAA-972<jats:sup>T</jats:sup>=CIP 108378<jats:sup>T</jats:sup>) and CDC 945<jats:sup>T</jats:sup> (=ATCC BAA-974<jats:sup>T</jats:sup>=CIP 108380<jats:sup>T</jats:sup>).</jats:p>

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