The Tomato<i>Aux</i>/<i>IAA</i>Transcription Factor<i>IAA9</i>Is Involved in Fruit Development and Leaf Morphogenesis

• Hua Wang

  Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France

• Brian Jones

  Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France

• Zhengguo Li

  Genetic Engineering Research Center  Chongqing University  40030 Chongqing  China

• Pierre Frasse

  Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France

• Corinne Delalande

  Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France

• Farid Regad

  Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France

• Salma Chaabouni

  Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France

• Alain Latché

  Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France

• Jean-Claude Pech

  Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France

• Mondher Bouzayen

  Unité Mixte de Recherche 990  Institut National de la Recherche Agronomique/Institut National Polytechnique–Ecole Nationale Supérieure Agronomique Toulouse  Génomique et Biotechnologie des Fruits Pôle de Biotechnologie Végétale  31326 Castanet-Tolosan Cedex  France

抄録

Auxin/indole-3-acetic acid (Aux/IAA) proteins are transcriptional regulators that mediate many aspects of plant responses to auxin. While functions of most Aux/IAAs have been defined mainly by gain-of-function mutant alleles in Arabidopsis thaliana, phenotypes associated with loss-of-function mutations have been scarce and subtle. We report here that the downregulation of IAA9, a tomato (Solanum lycopersicum) gene from a distinct subfamily of Aux/IAA genes, results in a pleiotropic phenotype, consistent with its ubiquitous expression pattern. IAA9-inhibited lines have simple leaves instead of wild-type compound leaves, and fruit development is triggered before fertilization, giving rise to parthenocarpy. This indicates that IAA9 is a key mediator of leaf morphogenesis and fruit set. In addition, antisense plants displayed auxin-related growth alterations, including enhanced hypocotyl/stem elongation, increased leaf vascularization, and reduced apical dominance. Auxin dose–response assays revealed that IAA9 downregulated lines were hypersensitive to auxin, although the only early auxin-responsive gene that was found to be upregulated in the antisense lines was IAA3. The activity of the IAA3 promoter was stimulated in the IAA9 antisense genetic background, indicating that IAA9 acts in planta as a transcriptional repressor of auxin signaling. While no mutation in any member of subfamily IV has been reported to date, the phenotypes associated with the downregulation of IAA9 reveal distinct and novel roles for members of the Aux/IAA gene family.

収録刊行物

• The Plant Cell

  The Plant Cell 17 (10), 2676-2692, 2005-08-26

  Oxford University Press (OUP)

キーワード

• Cell Biology
• Plant Science

詳細情報

• CRID: 1363107371032226304
• NII論文ID: 80017558369
• DOI: 10.1105/tpc.105.033415
• ISSN: 1532298X
• ISSN: http://id.crossref.org/issn/10404651
• Web Site: http://academic.oup.com/plcell/article-pdf/17/10/2676/36877318/plcell_v17_10_2676.pdf
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