Multiple incorporation of non‐natural amino acids into a single protein using tRNAs with non‐standard structures

• Takashi Ohtsuki

• Taishi Manabe

• Masahiko Sisido

抄録

The ability to introduce non‐natural amino acids into proteins opens up new vistas for the study of protein structure and function. This approach requires suppressor tRNAs that deliver the non‐natural amino acid to a ribosome associated with an mRNA containing an expanded codon. The suppressor tRNAs must be absolutely protected from aminoacylation by any of the aminoacyl‐tRNA synthetases in the protein synthesizing system, or a natural amino acid will be incorporated instead of the non‐natural amino acid. Here, we found that some tRNAs with non‐standard structures could work as efficient four‐base suppressors fulfilling the above orthogonal conditions. Using these tRNAs, we successfully demonstrated incorporation of three different non‐natural amino acids into a single protein.

収録刊行物

• FEBS Letters

  FEBS Letters 579 (30), 6769-6774, 2005-11-21

  Wiley

キーワード

• Cell Biology
• Genetics
• Molecular Biology
• Biochemistry
• Structural Biology
• Biophysics

詳細情報

• CRID: 1361418520422236160
• NII論文ID: 80017666919
• DOI: 10.1016/j.febslet.2005.11.010
• ISSN: 18733468
• ISSN: 00145793
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2005.11.010
• Web Site: https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2005.11.010
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