5'-Triphosphate RNA Is the Ligand for RIG-I
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- Veit Hornung
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Jana Ellegast
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Sarah Kim
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Krzysztof Brzózka
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Andreas Jung
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Hiroki Kato
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Hendrik Poeck
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Shizuo Akira
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Karl-Klaus Conzelmann
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Martin Schlee
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Stefan Endres
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
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- Gunther Hartmann
- Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany.
Abstract
<jats:p>The structural basis for the distinction of viral RNA from abundant self RNA in the cytoplasm of virally infected cells is largely unknown. We demonstrated that the 5′-triphosphate end of RNA generated by viral polymerases is responsible for retinoic acid–inducible protein I (RIG-I)–mediated detection of RNA molecules. Detection of 5′-triphosphate RNA is abrogated by capping of the 5′-triphosphate end or by nucleoside modification of RNA, both occurring during posttranscriptional RNA processing in eukaryotes. Genomic RNA prepared from a negative-strand RNA virus and RNA prepared from virus-infected cells (but not from noninfected cells) triggered a potent interferon-α response in a phosphatase-sensitive manner. 5′-triphosphate RNA directly binds to RIG-I. Thus, uncapped 5′-triphosphate RNA (now termed 3pRNA) present in viruses known to be recognized by RIG-I, but absent in viruses known to be detected by MDA-5 such as the picornaviruses, serves as the molecular signature for the detection of viral infection by RIG-I.</jats:p>
Journal
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- Science
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Science 314 (5801), 994-997, 2006-11-10
American Association for the Advancement of Science (AAAS)
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Keywords
Details 詳細情報について
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- CRID
- 1364233269629339776
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- NII Article ID
- 80018776801
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- ISSN
- 10959203
- 00368075
- http://id.crossref.org/issn/00368075
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- Data Source
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- Crossref
- CiNii Articles
- KAKEN