Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome

DOI Web Site 被引用文献9件
  • Motoko Ikeda
    Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
  • Masamitsu Shikata
    Shimadzu Corporation, Nakagyou, Kyoto 604-8511, Japan
  • Noriko Shirata
    Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
  • Sudawan Chaeychomsri
    Central Laboratory and Greenhouse Complex, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
  • Michihiro Kobayashi
    Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan

抄録

<jats:p>The whole-genome sequence of the <jats:italic>Hyphantria cunea</jats:italic> nucleopolyhedrovirus (HycuNPV) was analysed. The entire nucleotide sequence of the HycuNPV genome was 132 959 bp long, with a G+C content of 45.1 mol%. A total of 148 open reading frames (ORFs) consisting of more than 50 aa were encoded by the genome. HycuNPV shares more than 122 ORFs with other lepidopteran group I NPVs, including <jats:italic>Autographa californica MNPV</jats:italic>, <jats:italic>Bombyx mori NPV</jats:italic>, <jats:italic>Choristoneura fumiferana MNPV</jats:italic> (CfMNPV), <jats:italic>Choristoneura fumiferana</jats:italic> defective NPV, <jats:italic>Epiphyas postvittana MNPV</jats:italic> and <jats:italic>Orgyia pseudotsugata MNPV</jats:italic> (OpMNPV). Six ORFs are identified as being unique to HycuNPV. Most of the HycuNPV ORFs showed higher similarity to CfMNPV and OpMNPV ORFs than to those of the other group I NPVs. HycuNPV encodes two conotoxin-like homologues (<jats:italic>ctl</jats:italic>s), which are observed only in OpMNPV in group I NPVs. HycuNPV encodes three inhibitors of apoptosis (<jats:italic>iap</jats:italic>s), <jats:italic>hycu-iap-1</jats:italic>, <jats:italic>hycu-iap-2</jats:italic> and <jats:italic>hycu-iap-3</jats:italic>, a feature that it shares only with CfMNPV. In addition, six homologous regions (<jats:italic>hr</jats:italic>s) are identified in the HycuNPV genome. These <jats:italic>hr</jats:italic>s are located in regions similar to those of the OpMNPV <jats:italic>hr</jats:italic>s, but different from most of the CfMNPV <jats:italic>hr</jats:italic>s. Based on the close phylogenetic relationship and conservation of group I NPV-specific genes, such as <jats:italic>gp64</jats:italic>, <jats:italic>ie-2</jats:italic> and <jats:italic>ptp-1</jats:italic>, it is concluded that HycuNPV belongs to the group I NPVs and is most similar to CfMNPV or OpMNPV.</jats:p>

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