Characterization of the Class IV Homeodomain-Leucine Zipper Gene Family in Arabidopsis

  • Miyuki Nakamura
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060–0810, Japan (M.N., H.K., M.A., K.T.Y.); Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113–0033, Japan (N.Y., Y.K.); and Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700–8530, Japan (T.T.)
  • Hiroshi Katsumata
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060–0810, Japan (M.N., H.K., M.A., K.T.Y.); Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113–0033, Japan (N.Y., Y.K.); and Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700–8530, Japan (T.T.)
  • Mitsutomo Abe
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060–0810, Japan (M.N., H.K., M.A., K.T.Y.); Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113–0033, Japan (N.Y., Y.K.); and Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700–8530, Japan (T.T.)
  • Naoto Yabe
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060–0810, Japan (M.N., H.K., M.A., K.T.Y.); Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113–0033, Japan (N.Y., Y.K.); and Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700–8530, Japan (T.T.)
  • Yoshibumi Komeda
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060–0810, Japan (M.N., H.K., M.A., K.T.Y.); Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113–0033, Japan (N.Y., Y.K.); and Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700–8530, Japan (T.T.)
  • Kotaro T. Yamamoto
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060–0810, Japan (M.N., H.K., M.A., K.T.Y.); Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113–0033, Japan (N.Y., Y.K.); and Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700–8530, Japan (T.T.)
  • Taku Takahashi
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060–0810, Japan (M.N., H.K., M.A., K.T.Y.); Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113–0033, Japan (N.Y., Y.K.); and Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700–8530, Japan (T.T.)

抄録

<jats:title>Abstract</jats:title> <jats:p>The Arabidopsis (Arabidopsis thaliana) genome contains 16 genes belonging to the class IV homeodomain-Leucine zipper gene family. These include GLABRA2, ANTHOCYANINLESS2, FWA, ARABIDOPSIS THALIANA MERISTEM LAYER1 (ATML1), and PROTODERMAL FACTOR2 (PDF2). Our previous study revealed that atml1 pdf2 double mutants have severe defects in the shoot epidermal cell differentiation. Here, we have characterized additional members of this gene family, which we designated HOMEODOMAIN GLABROUS1 (HDG1) through HDG12. Analyses of transgenic Arabidopsis plants carrying the gene-specific promoter fused to the bacterial β-glucuronidase reporter gene revealed that some of the promoters have high activities in the epidermal layer of the shoot apical meristem and developing shoot organs, while others are temporarily active during reproductive organ development. Expression profiles of highly conserved paralogous gene pairs within the family were found to be not necessarily overlapping. Analyses of T-DNA insertion mutants of these HDG genes revealed that all mutants except hdg11 alleles exhibit no abnormal phenotypes. hdg11 mutants show excess branching of the trichome. This phenotype is enhanced in hdg11 hdg12 double mutants. Double mutants were constructed for other paralogous gene pairs and genes within the same subfamily. However, novel phenotypes were observed only for hdg3 atml1 and hdg3 pdf2 mutants that both exhibited defects in cotyledon development. These observations suggest that some of the class IV homeodomain-Leucine zipper members act redundantly with other members of the family during various aspects of cell differentiation. DNA-binding sites were determined for two of the family members using polymerase chain reaction-assisted DNA selection from random oligonucleotides with their recombinant proteins. The binding sites were found to be similar to those previously identified for ATML1 and PDF2, which correspond to the pseudopalindromic sequence 5′-GCATTAAATGC-3′ as the preferential binding site.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 141 (4), 1363-1375, 2006-06-15

    Oxford University Press (OUP)

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