Simultaneous mutations in translation initiation factors eIF4E and eIF(iso)4E are required to prevent pepper veinal mottle virus infection of pepper

DOI Web Site 被引用文献4件
  • Sandrine Ruffel
    INRA, Genetics and Breeding of Fruits and Vegetables, Domaine St Maurice, BP 94, F-84143 Montfavet, France
  • Jean-Luc Gallois
    INRA, Genetics and Breeding of Fruits and Vegetables, Domaine St Maurice, BP 94, F-84143 Montfavet, France
  • Benoît Moury
    Plant Pathology, Domaine St Maurice, BP 94, F-84143 Montfavet, France
  • Christophe Robaglia
    Laboratoire de Génétique et Biophysique des Plantes, CEA-CNRS-Université Aix-Marseille II, Faculté des Sciences de Luminy, F-13009 Marseille, France
  • Alain Palloix
    INRA, Genetics and Breeding of Fruits and Vegetables, Domaine St Maurice, BP 94, F-84143 Montfavet, France
  • Carole Caranta
    INRA, Genetics and Breeding of Fruits and Vegetables, Domaine St Maurice, BP 94, F-84143 Montfavet, France

抄録

<jats:p><jats:italic>Capsicum</jats:italic>resistance to<jats:italic>Pepper veinal mottle virus</jats:italic>(PVMV) results from complementation between the<jats:italic>pvr2</jats:italic>and<jats:italic>pvr6</jats:italic>resistance genes: recessive alleles at these two loci are necessary for resistance, whereas any dominant allele confers susceptibility. In line with previous results showing that<jats:italic>pvr2</jats:italic>resistance alleles encode mutated versions of the eukaryotic translation initiation factor 4E (eIF4E), the involvement of other members of the<jats:italic>eIF4E</jats:italic>multigenic family in PVMV resistance was investigated. It was demonstrated that<jats:italic>pvr6</jats:italic>corresponds to an<jats:italic>eIF(iso)4E</jats:italic>gene, predicted to encode the second cap-binding isoform identified in plants. Comparative genetic mapping in pepper and tomato indicated that<jats:italic>eIF(iso)4E</jats:italic>maps in the same genomic region as<jats:italic>pvr6</jats:italic>. Sequence analysis revealed an 82 nt deletion in<jats:italic>eIF(iso)4E</jats:italic>cDNAs from genotypes with the<jats:italic>pvr6</jats:italic>resistance allele, leading to a truncated protein. This deletion was shown to co-segregate with<jats:italic>pvr6</jats:italic>in doubled haploid and F<jats:sub>2</jats:sub>progeny. Transient expression in a PVMV-resistant genotype of<jats:italic>eIF(iso)4E</jats:italic>derived from a genotype with the<jats:italic>pvr6</jats:italic><jats:sup>+</jats:sup>susceptibility allele resulted in loss of resistance to subsequent PVMV inoculation, confirming that<jats:italic>pvr6</jats:italic>encodes the translation factor eIF(iso)4E. Similarly, transient expression of<jats:italic>eIF4E</jats:italic>from a genotype with the<jats:italic>pvr2</jats:italic><jats:sup>+</jats:sup><jats:italic>-eIF4E</jats:italic>susceptibility allele also resulted in loss of resistance, demonstrating that wild-type eIF4E and eIF(iso)4E are susceptibility factors for PVMV and that resistance results from the combined effect of mutations in the two cap-binding isoforms. Thus, whilst most potyviruses specifically require one eIF4E isoform to perform their replication cycle, PVMV uses either eIF4E or eIF(iso)4E for infection of pepper.</jats:p>

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