Visualization of Cellulose Synthase Demonstrates Functional Association with Microtubules

DOI Web Site 被引用文献59件
  • Alexander R. Paredez
    Department of Biological Sciences, Stanford University, 260 Panama Street, Stanford, CA 94305, USA.
  • Christopher R. Somerville
    Department of Biological Sciences, Stanford University, 260 Panama Street, Stanford, CA 94305, USA.
  • David W. Ehrhardt
    Department of Biological Sciences, Stanford University, 260 Panama Street, Stanford, CA 94305, USA.

抄録

<jats:p> Expression of a functional yellow fluorescent protein fusion to cellulose synthase (CESA) in transgenic <jats:italic>Arabidopsis</jats:italic> plants allowed the process of cellulose deposition to be visualized in living cells. Spinning disk confocal microscopy revealed that CESA complexes in the plasma membrane moved at constant rates in linear tracks that were aligned and were coincident with cortical microtubules. Within each observed linear track, complex movement was bidirectional. Inhibition of microtubule polymerization changed the fine-scale distribution and pattern of moving CESA complexes in the membrane, indicating a relatively direct mechanism for guidance of cellulose deposition by the cytoskeleton. </jats:p>

収録刊行物

  • Science

    Science 312 (5779), 1491-1495, 2006-06-09

    American Association for the Advancement of Science (AAAS)

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