DNA cloning : a practical approach

著者

• Glover, David M.

書誌事項

DNA cloning : a practical approach

edited by D.M. Glover

（The practical approach series）

IRL Press, c1985-

• 1
• 2
• 3 : softbound
• 3 : Hardbound

注記

Includes bibliographies and indexes

内容説明・目次

巻冊次

1 ISBN 9780947946180

内容説明

Techniques in molecular biology continue to develop and find wider applications both in research and the biotechnology industry. DNA Cloning aims to extend and complement existing cloning manuals. Contents of Volume I range from the use of phage lamda replacement vectors in the construction of representative genomic DNA libraries to broad host range cloning vectors for Gram-negative bacteria.

目次

• The use of phage lambda replacement vectors in the construction of representative genomic DNA libraries
• Constructing and screening cDNA libraries in gt10 and gt11
• An alternative procedure for the synthesis of double-stranded cDNA for cloning in phage and plasmid vectors
• Immunological detection of chimeric B-galactosidases expressed by plasmid vectors
• The pEMBL family of single-stranded vectors
• Techniques for transformation of E.Coli
• The use of genetic markers for the selection and allelic exchange of in vitro induced mutations that do not have a phenotype in E. Coli
• The oligonucleotide-directed construction of mutations in recombinant filamentous phage
• Broad host range cloning vectors for gram negative bacteria

巻冊次

2 ISBN 9780947946197

目次

• Application of plasmids containing promoters specific for phage-encoded RNA polymerases
• Choice and use of cosmid vectors
• Genetic approaches to the cloning, modification and characterization of cosmid clones and clone libraries
• The purification of eukaryotic polypeptides expressed in escherichia coli
• Production and purification of polyclonal antibodies to the foreign segment of B-Galactosidase fusion proteins
• Production of monoclonal antibodies against fusion proteins produced in escherichia coli
• Expression and secretion of foreign genes in yeast
• The use of vectors based on gene amplification for the expression of cloned genes in mammalian cells
• Retroviral vectors
• The production of transgenic mice by the microinjection of cloned DNA into fetilized one-cell eggs

巻冊次

3 : softbound ISBN 9781852210489

内容説明

This volume presents for the first time in a single book a variety of current techniques for cloning and expressing DNA molecules. Protocols are described in sufficient detail for immediate use in the laboratory.

目次

• Bacillus cloning methods
• Gene cloning in streptomyces
• Cloning in yeast
• Genetic Engineering of plants
• P element mediated germ line transformation of drosophila
• High efficiency gene transfer into mammalian cells
• The construction and characterization of vaccinia virus recombinants expressing foreign genes
• Bovine papilloma virus DNA: A eukaryotic cloning vector

巻冊次

3 : Hardbound ISBN 9781852210496

目次

• Bacillus cloning methods
• Gene cloning in streptomyces
• Cloning in yeast
• Genetic Engineering of plants
• P element mediated germ line transformation of drosophila
• High efficiency gene transfer into mammalian cells
• The construction and characterization of vaccinia virus recombinants expressing foreign genes
• Bovine papilloma virus DNA: A eukaryotic cloning vector

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BA00467251
• ISBN:
  • 0947946187
  • 0947946195
  • 1852210486
  • 1852210494
• LCCN: 85202319
• 出版国コード: uk
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Oxford ; Washington, DC
• ページ数/冊数: v.
• 大きさ: 23 cm
• 分類:
  • LCC : QH442.2
  • DC19 : 574.87/3282
• 件名:
  • LCSH : Molecular cloning
  • LCSH : Recombinant DNA
• 親書誌ID:
  • BA00227768