Nucleic acids and related compounds

著者

書誌事項

Nucleic acids and related compounds

editor, Ante M. Krstulović

（CRC series in chromatography, . CRC handbook of chromatography / editors in chief, Gunter Zweig, Joseph Sherma/）

CRC Press, c1987

  • vol.1 pt.A
  • vol.1 pt.B

大学図書館所蔵 件 / 全8件

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注記

Includes bibliographies and index

内容説明・目次

巻冊次

vol.1 pt.A ISBN 9780849330209

内容説明

This handbook provides a comprehensive review of the most current developments in nucleic acid research by leading scientists worldwide. Chapters focus on structure elucidation methods, fundamentals of HPLC; analysis of nucleic acids and oligonucleotides; analysis of polynucleotides, nucleotides, nucleosides and bases; nucleic acid components in biological fluids and their diagnostic value; and miscellaneous application. The format is organized according to chromatographic techniques and by chemical classifications. Each subject is presented in the form of a critical review.

目次

PART A: Structure Elucidation of Nucleic Acid Components. Fundamentals of HPLC. Analysis of Nucleic Acids and Oligonucleotides. Extraction of Nucleic Acids from Tissues. Gel Filtration Chromatography of RNAs and DNS Fragments. Separation of tRNAs and Oligonucleotides by Mixed Mode Chromatography. Anion-Exchange and Reversed-Phase HPLC of Synthetic Oligonucleotides. Nucleic Acid Components in Biological Fluids. RPLC Separation of RNA and DNA Hydrolysates. Nucleotides in Tissue Extracts. Determination of Adenine Nucleotides and Creatine Phosphate in Various Mammalian Tissues. HPLC Determination of "Unusual Nucleotides" (bis[5*-nucleosidyl] oligophosphates and guanosine 5*, 3*-bispolyphosphates)I in Cellular Extracts. Nucleosides and Bases in Extracellular Fluids Analyzed by HPLC. Nucleic Acid Components and Related Compounds in Uremic Hemodialysates Analyzed by HPLC. Screening for Inborn Errors of Purine and Pyrimidine Metabolism by Bidimensional TLC and HPLC. Anion-Exchange Analysis of Isotopically Labeled Nucleotides, Nucleosides, and Bases in Metabolic Disorders. HPLC Determination of Enzyme Activities of Nucleotide/Nucleoside Metabolism. Index.
巻冊次

vol.1 pt.B ISBN 9780849330216

内容説明

This handbook provides a comprehensive review of developments in nucleic acid research by leading scientists worldwide. Chapters focus on structure elucidation methods, fundamentals of HPLC; analysis of nucleic acids and oligonucleotides; analysis of polynucleotides, nucleotides, nucleosides and bases; nucleic acid components in biological fluids and their diagnostic value; and miscellaneous application. The format is organized according to chromatographic techniques and by chemical classifications. Each subject is presented in the form of a critical review.

目次

PART B: Analysis of Polynucleotides, Nucleotides, Nucleosides, and Bases. Isolation and Extraction of Nucleotides from Tissues Prior to HPLC. Purification of Plant Nucleotide Extracts Prior to HPLC Analysis. Optimization and Prediction of Retention of Nucleic Acid Components Separated by HPLC. Separation of Nucleobases and Nucleosides on Silica Gel. The Liquid Chromatographic Behavior of Outer-Sphere and Inner-Sphere Complexes of Nucleosides and Nucleotides with Metal Ions. Separations of Nucleic Acid Components by Bidimensional HPLC. Simultaneous Analysis of Nucleotides, Nucleosides, and Bases by Ion Exchange and On-Line Exchange-Reversed Phase Chromatography. Analysis of Urinary and Plasma Nucleosides by Column Switching Technique. Labeling of Pyrimidine Compounds for Fluorescent Detection in HPLC. Continuous Flow UV-VIS Scanning of Nucleic Acid Components Separated by HPLC. Planar Chromatography and Its Applications to Nucleic-Acid Research. Miscellaneous Applications. HPLC of Pharmacologically Important Nucleobase and Nucleoside Analogs: 6-Thioguanine, 5-Fluorouracil, and Cytarabine. HPLC Investigation of PT (III) Coordination Complexes with Nucleic Acid Components. Index.

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