Neuropeptides : a methodology
著者
書誌事項
Neuropeptides : a methodology
（IBRO handbook series : methods in the neurosciences, v. 11）
Wiley, c1989
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注記
"A Wiley-Interscience publication."
Includes bibliographies and index
内容説明・目次
内容説明
This book focuses on a detailed description of modern methods for the study of neuropeptides used in leading laboratories. Specific topics discussed include molecular genetic techniques, in_situ hybridization, immunocytochemistry, ultrastructure morphometry, and measurement of neuropeptide release in_vivo and in_vitro. The book also covers biochemical and electrophysiological (including patch clamp) techniques for investigating neuropeptide-receptor interactions and second messenger systems. These cellular and biochemical approaches lead finally to a review of methods for studying whole animal behaviour. Focusing on a detailed description of modern methods used in laboratory studies, the book examines biochemical and electrophysiological techniques and discusses such topics as molecular genetic techniques, in situ hybridization and immunocytochemistry. The text concludes with a review of methods for studying whole animal behaviour.
目次
- Chemical synthesis of peptides
- characterisation of cdnas encoding neuropeptide precursors
- in_situ hybridization in the vertebrate nervous system
- strategies for the ultrastructural study of peptide-containing neurones
- separation and determination of neuropeptides using hplc with electrochemical detection
- release of neuropeptides in_vitro
- neuropeptide release in_vivo - measurement in hypophysial portal blood
- neuropeptide-degrading enzymes
- characterisation of neuropeptide receptors using photoaffinity and biotinylated analogs
- second messenger responses to neuropeptides.
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