Laboratory methods in immunology

著者

書誌事項

Laboratory methods in immunology

editor, Heddy Zola

CRC Press, c1990

  • v. 1
  • v. 2

大学図書館所蔵 件 / 全19件

この図書・雑誌をさがす

注記

Includes bibliographies references and index

内容説明・目次

巻冊次

v. 1 ISBN 9780849344817

内容説明

This two-volume reference details immunological techniques for biologists of all disciplines. Volume I includes a detailed discussion of the tissue culture laboratory. It addresses what the lab needs to be, and the general "housekeeping" procedures involved in tissue culture. Presented next are chapters on specific aspects of tissue culture and hybridoma technology. The book includes a review of bioassays for interleukins, and a series of papers on lymphokines and functional assays in vitro. The section on molecular genetic studies begins with consideration of the choice of strategies for cloning the genes of cell surface molecules. It continues with papers on aspects of molecular biological techniques most closely related to immunology. The final section covers immunochemical techniques. Volume II reviews techniques used with small laboratory animals. It includes papers on specialized procedures with animals. Technical aspects are emphasized through a detailed analysis of effects of ultraviolet light on the immune system. Covered also is antigen detection in cells and tissue. The book addresses the important areas of protein purification using monoclonal antibodies.These two volumes are of great importance to those who use immunological techniques, whether they are immunologists or trained in other disciplines. The book is intended for those in animal science, veterinary science, genetics and cell biology, bacteriology, immunology, pathology, biochemistry, laboratory medicine, and hematology.

目次

VOLUME I: TISSUE CULTURE, HYBRIDOMAS. Housekeeping for Cell Culture Laboratories. Growing Hybridomas in Dialysis Tubing: Optimization of the Technique. The Production of Human Monoclonal Antibodies by In Vitro Immunization. Class Switching of Monoclonal Antibodies. Purification of Mouse Monoclonal Antibodies from Ascitic Fluid and Culture Supernatants by Precipitation with Polyethylene Glycol 6000. LYMPHOKINES, IN VITRO FUNCTIONAL ASSAYS. Bioassays for Interleukins. Preparation of Human Tonsil B Lymphocytes by a two-stage Negative Selection Procedure. Detection and Enumeration of Single Antibody- or Cytokine-Secreting Cells by the ELISA-Plaque (ELISPOT) Assay. Detection and Enumeration of Rheumatoid Factor-Secreting Cells. A Radiometric Assay for Simultaneous Measurement of Ingestion and Killing by Human Phagocytes. Flow-Cytometric Measurement of Respiratory Burst Activity of Neutrophils. An Assay for the Measurement of Attachment of Inflammatory Cells to Endothelial-Monolayers and to Plastic Surfaces. MOLECULAR GENETIC STUDIES. The Cloning of Genes and cDNAs Coding for Cell Membrane Antigenic Determinants. Chimeric Monoclonal Antibodies Composed of Mouse-Derived V Regions and Human-Derived C Regions. Identification of Antigens Recognized by T Cells Using Western Blot and Gene Cloning Techniques. Site-Directed Mutagenesis to Study Protein Structure and Function. IMMUNOCHEMISTRY. Determination of Antibody Affinity and Concentration by Solution-Phase Microradioimmunoassay. Measurement of Gluten Antibody Isotypes. Measurement of Cardiolipin Antibodies. Development of Quantitative Two-Site ELISAs for Soluble Proteins. Determination of Membrane-Associated Immunoglobulins and Complement C3d of Human Blood Cells by a Competitive Enzyme-Linked Immunoassay (CELIA). Monoclonal Antibody-Specific Immobilization of Membrane Constituents for the Detection of Platelet Antigens and Antibodies. Index,
巻冊次

v. 2 ISBN 9780849344824

内容説明

This two-volume reference details immunological techniques for biologists of all disciplines. Volume I includes a detailed discussion of the tissue culture laboratory. It addresses what the lab needs to be, and the general "housekeeping" procedures involved in tissue culture. Presented next are chapters on specific aspects of tissue culture and hybridoma technology. The book includes a review of bioassays for interleukins, and a series of papers on lymphokines and functional assays in vitro. The section on molecular genetic studies begins with consideration of the choice of strategies for cloning the genes of cell surface molecules. It continues with papers on aspects of molecular biological techniques most closely related to immunology. The final section covers immunochemical techniques. Volume II reviews techniques used with small laboratory animals. It includes papers on specialized procedures with animals. Technical aspects are emphasized through a detailed analysis of effects of ultraviolet light on the immune system. Covered also is antigen detection in cells and tissue. The book addresses the important areas of protein purification using monoclonal antibodies.These two volumes are of great importance to those who use immunological techniques, whether they are immunologists or trained in other disciplines. The book is intended for those in animal science, veterinary science, genetics and cell biology, bacteriology, immunology, pathology, biochemistry, laboratory medicine, and hematology.

目次

VOLUME II: ANIMAL WORK, IN VIVO STUDIES. Basic Techniques with Experimental Animals. Immunological Adjuvants: General Properties, Advantages, and Limitations. Labeling of Lymphocytes for In Vivo Tracing. Photoimmunology: Ultraviolet Radiation Effects on the Immune System. ANTIGEN DETECTION IN TISSUES AND CELLS. Ultrastructural Demonstration of Cell Surface and Intracellular Class II Major Histocompatibility Antigens by Monoclonal Antibodies Using a Pre-Embedding Indirect Immunoperoxidasae Technique. A Microplate ELISA for the Detection of Internal and External Cellular Antigens. IMMUNOAFFINITY PURIFICATION. The Use of Immunoaffinity Chromatography in the Preparation of Therapeutic Products. Methodology for the Immunopurification and Immunodepletion of Coagulation Factors, with Emphasis on Factors VIII and IX. Improved Cyanogen Bromide Activation. A User Guide to Protein A. Immunoaffinity Adsorption: Practical Application in Laboratory Research and Production. Production and Characterization of Solid-Phase Immunoadsorbents. Index. Catalog no. 4482...Approx. U.S. $124.94/Outside U.S. $147.00.

「Nielsen BookData」 より

詳細情報

ページトップへ