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v. 1 : Berlin ISBN 9783540130345
内容説明
A puzzling epidemiological problem was the driving force behind the discovery of human adenoviruses by Wallace Rowe and his colleagues 30 years ago. The de- velopment of a plaque assay for poliomyelitis virus in 1953 led us to the threshold of quantitative virology, and in the same year the double-helical structure of DNA was discovered and became a cornerstone of mo- lecular biology. The potential of adenoviruses as research tools in the molecular and cellular biology of eukaryotic cells was recognized as early as the late 1950s and early 1960s by several investigators. Structural and biochemical stu- dies dominated the early years. In 1962, some of the adenoviruses were the first human viruses shown to be oncogenic in experimental animals. Thus adenovirology offered the investigator the entire gamut of host cell interactions, productive and abortive, as well as trans- formed and tumor cell systems. The possibilities that adenoviruses afforded for the study of the molecular biology and genetics of eukaryotic cells were fully rea- lized in the late 1960s and the 1970s.
目次
Structure and Assembly of Adenoviruses. With 6 Figures.- The Mechanism of Adenovirus DNA Replication and the Characterization of Replication Proteins. With 8 Figures.- The Origin of Adenovirus DNA Replication. With 3 Figures.- A New Mechanism for the Initiation of Replication of ?29 and Adenovirus DNA: Priming by the Terminal Protein. With 6 Figures.- The Messenger RNAs from the Transforming Region of Human Adenoviruses. With 4 Figures.- In Vitro Transcription of Adenovirus Genes. With 9 Figures.- Adenovirus Early Gene Regulation and the Adeno-associated Virus Helper Effect. With 10 Figures.- Antibodies to Synthetic Peptides Targeted to the Transforming Genes of Human Adenoviruses - An Approach to Understanding Early Viral Gene Function. With 6 Figures.- On the Mechanism of Recombination Between Adenoviral and Cellular DNAS: The Structure of Junction Sites. With 17 Figures.
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v. 2 : Berlin ISBN 9783540131274
内容説明
The data summarized in this chapter show that morphological transformation and oncogenesis by adenoviruses are brought about by the coordinated activity of regions E1A and E1B. Gene products of each of these subregions appear to fulfill distinct roles in oncogenic transformation, with the possible exception of the product(s) encoded by the O. 9-kb E1A mRNA. Also unclear is the func- tion of the 20-kd E1B protein, which has a small role, if any, in morphological transformation, but appears to be essential for the development of the oncogenic phenotype, as defined by the ability of transformed cells to grow in immuno- deficient nude mice. The differences in biological properties of oncogenic and nononcogenic adenoviruses must be attributed to differences in the primary structure of the respective E1A and E1B gene products, in particular of the product(s) of the 1. 0-kb E1A mRNA and of the 55-kd protein encoded by the 2. 2-kb EiB mRNA.
The availability of cold-sensitive adenovirus mutants has enabled us to conclude that the transformed phenotype is maintained as a result of continuous expression of at least region E1A gene products, and is therefore not the result of a hit-and-run mechanism. Despite the progress in our understanding of adenovirus transformation and oncogenesis, virtually nothing is known about the precise mechanism by which the viral gene products bring about the neoplastic changes in cells. The only exception is the demonstration that Ad12 region E1A (1.
目次
The Interface Between Adenovirus-Transformed Cells and Cellular Immune Response in the Challenged Host.- Transformation and Oncogenicity by Adenoviruses.- Organization, Integration, and Transcription of Transforming Genes of Oncogenic Human Adenovirus Types 12 and 7.- Nucleotide Sequences of Adenovirus DNAs.- The Adenovirus Early Proteins.- Molecular Biology of S16 (SA7) and Some Other Simian Adenoviruses.- Molecular Epidemiology of Human Adenoviruses.- The In Vitro Replication of Adenovirus DNA.- Erratum to the Chapter by Doerfler et al.: On the Mechanism of Recombination Between Adenoviral and Cellular DNAs: The Structure of Junction Sites. In: Current Topics in Microbiology and Immunology, Vol. 109.
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v. 3 : Berlin ISBN 9783540131380
目次
Genetic Analysis of Adenoviruses.- Adenovirus DNA Replication.- A Study of Viral Genomes in Cells Transformed by the Nononcogenic Human Adenovirus Type 5 and Highly Oncogenic Bovine Adenovirus Type 3.- Early and Late Proteins of Adenovirus Type 12: Translation Mapping with RNA Isolated from Infected and Transformed Cells.
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