Methods in nucleic acids research
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書誌事項
Methods in nucleic acids research
CRC Press, c1991
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Methods in Nucleic Acids Research provides extensively referenced overviews of chapter topics, in addition to step-by-step laboratory protocols. Topics include discussions regarding the preparation and assay of antibodies against oligopeptides, RNA footprinting, gel-retardation assays for nucleic acid binding proteins, in vitro transcription and translation assays for studies of eukaryotic gene expression, human genome mapping, forensic analysis of DNA polymorphism, in situ hybridization for the detection of specific RNA, and other methods. Biochemists, molecular biologists, immunologists, cell biologists, and geneticists will find this book invaluable for their research.
目次
PREPARATIVE METHODS. Purification of Chromosomal DNA for Library Construction. The Chemical Synthesis and Some Uses of Oligonucleotides in Biological Research. In Vitro Site-Directed Mutagenesis. Antibodies Against Oligopeptides: Preparation and Assay. Polymerase Chain Reaction to Amplify DNA from Phage Plaques. DNA AND GENOMIC ANALYSIS (ELECTROPHORETIC METHODS). Protocols for Manual Dideoxy DNA Sequencing. Pulsed Field Electrophoreses: Procedures, Problems, and Practicalities. Mapping the Human Genome. Fragment Length Polymorphisms for Forensic Science Applications. PROTEIN-NUCLEIC ACID INTERACTIONS. Gel Retardation Assays for Nucleic Acid-Binding Proteins. DNA Footprinting Techniques: Applications to Eukaryotic Nuclear Proteins. RNA Footprinting. GENE EXPRESSION. Plasmid Cloning Vectors for the Overproduction of Proteins in Escherichia coli. Transfection of Cultured Mammalian Cells by Mammalian Expression Vectors. Immunoscreening of cDNA Expression Libraries. In Vitro Transcription Assays for Eukaryotic Gene Expression. Measuring Eukaryotic Gene Expression In Vitro: Translation Assays. Detection of Specific RNA Sequences in Tissue Sections by In Situ Hybridization. Appendix: Sources of Reagents and Supplies for Dideoxy DNA Sequencing and Other Applications.
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