Animal cell culture methods
著者
書誌事項
Animal cell culture methods
（Methods in cell biology / edited by David M. Prescott, v. 57）
Academic Press, c1998
- : hbk
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780124800403
内容説明
This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques.
目次
Principles of Cell Culture:
D. Barnes, Animal Cell Culture Equipment and Techniques.
J.P. Mather, Making Informed Choices: Medium, Serum, and Serum-free Medium. How to Choose the Appropriate Medium and Culture System for the Model You Wish to Create.
R.J. Hay, Cell Line Availability: Where to Get the Cell Lines You Need.
C.K. Lincoln and M.G. Gabridge, Cell Culture Contamination: Sources, Consequences, Prevention, and Elimination. Establishing Cell Lines:
Y. Katakura, S. Alam, and S. Shirahata, Immortalization by Gene Transfection.
M.-C. Hofmann and J.L. Millan, Establishment of Mammalian Testicular Cell Lines.
S. Shirahata, Y. Katakura, and K. Teruya, Cell Hybridization, Hybridomas, and Human Hybridomas.
M. Westphal and H. Meissner, Establishing Human Glioma-Derived Cell Lines.
R. Li, Culture Methods for Selective Growth of Normal Rat and Human Schwann Cells.
C.J. Bayne, Invertebrate Cell Culture Considerations: Insects, Ticks, Shellfish, and Worms.
J. Kaplan and B. Hukku, Cell Line Characterization and Authentication.
Specialized Culture Techniques:
J.P. Mather, Laboratory Scale-up of Cell Cultures (0.5-50 Liters).
G.F. Merrill, Cell Synchronization.
D.T. Loo and J.R. Tillema, Measurement of Cell Death.
A. Moore, C.J. Donahue, K.D. Bauer, and J.P. Mather, Simultaneous Measurement of Cell Cycle and Apoptotic Cell Death.
M. Pirity, A.-K. Hadjantonakis, and A. Nagy, Embryonic Stem Cells, Creating Transgenic Animals.
Microscopy and Morphology:
D.M. Phillips, Electron Microscopy: Use of Transmission and Scanning Electron Microscopy to Study Cells in Culture.
S.P. Wheatly and Y.-L. Wang, Indirect Immunofluorescence Microscopy in Cultured Cells.
T.K. Woodruff, Cellular Localization of mRNA and Protein: In Situ Hybridization Histochemistry and In Situ Ligand Binding.
Subject Index.
- 巻冊次
-
: hbk ISBN 9780125441599
内容説明
This volume in the series provides coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific study, as well as analytical and procedural techniques.
目次
- Preface, L.M. Schwartz and B.A. Osbourne
- Anatomical Methods in Cell Death, J.F.R. Kerr, G.C. Gobe, C.M. Winterford, and B.V. Harmon
- Identification of Dying Cells - In Situ Staining, S.A. Ben-Sasson and Y. Gavrieli
- Assays of DNA Fragmentation, Endonucleases, and Intracellular pH and Ca2+ Associated with Apoptosis, A. Eastman
- Quantitation of Apoptotic Events in Pure and Heterogeneous Populations of Cells using the Flow Cytometer, P.J. Fraker, L.E. King, D. Lill-Elghanian, and W.G. Telford
- Cell Cycle Analysis of Apoptosis Using Flow Cytometry, S.W. Sherwood and R.T. Schimke
- Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes, B.A. Osborne, S. Smith, S.-G. Liu, K. McLaughlin, and L. M. Schwartz
- Cloning Cell Death Genes, L.M. Schwartz, C.E. Milligan, W. Bielke, and S.J. Robinson
- Use of the Yeast Two-Hybrid System for Identifying the Cascade of Protein Interactions Resulting in Apoptotic Cell Death, L.T. Bemis, F.J. Geske, and R. Strange
- The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro, A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J. Mogil, and D.R. Green
- Calcium, Free Radicals, and Excitotoxic Neuronal Death in Primary Cell Culture, M.P. Mattson, S.W. Barger, J.G. Begley, and R.J. Mark
- Use of Cultured Neurons and Neuronal Cell Lines to Study Morphological, Biochemical, and Molecular Changes Occurring in Cell Death, J.C. Mills, S. Wang, M. Ericinska, and R.N. Pittman
- Methods for Studying Cell Death and Viability of Primary Neuronal Cultures, J.E. Johnson
- Neuron Death in Vertebrate Development: In Vivo Methods, P.G.H. Clarke and R.W. Oppenheim
- Methods for the Study of Cell Death in the Nematode Caenorhabditis elegans, M. Driscoll
- Programmed Cell Death during Mammary Gland Involution, R. Strange, R.R. Friis, L.T. Bemis, and F.J. Geske
- Methods for the Study of Apoptosis in the Ventral Prostate Gland, R. Buttyan
- Genetic Approaches for Studying Programmed Cell Death during Development of the Laboratory Mouse, E.C. Coucouvanis, G.R. Martin, and J.H, Nadeau
- Subject Index.
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