Polyamine protocols

著者

書誌事項

Polyamine protocols

edited by David M.L. Morgan

（Methods in molecular biology / John M. Walker, series editor, 79）

Humana Press, c1998

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注記

Includes index and bibliographical references

内容説明・目次

内容説明

Putrescine and spermidine are ubiquitous in living organisms. Spermine, third of the three most commonly occurring natural polyamines, is probably present in all eukaryotes but is rare (or nonexistent) in prokaryotes. Polyamine residues are constituents of many compounds found in plants and insects. Putrescine, spermidine, or spermine-containing alkaloids are found in many plants, nonproteinaceous spider and wasp toxins contain polyamine residues, and glutathionyl-spermidine conjugates have been found in some pathogenic microorganisms. In most cells polyamines are the products of a highly regulated bios- thetic pathway. It is not clear whether the elaborate regulation of polyamine synthesis is a consequence of their essential role(s) in cellular differentiation and development, or part of a defense mechanism to prevent overaccumulation of compounds that are toxic in excess. In addition to their biosynthetic capa- bility, many cells also possess transport systems for polyamines that respond to intracellular polyamine levels, and other stimuli, and are regulated by mecha- nisms that are at present incompletely defined. Two routes of polyamine catabolism have been identified in mammalian cells, a biodegradative route and a recycling pathway. The relative impor- tance of these pathways and their overall regulation is only partially resolved. What is clear is the widespread occurrence of a variety of polyamine-oxid- ing enzymes in animals, plants, bacteria, and fungi. Polyamine catabolism, by whichever route, results in the formation of aminoaldehydes as intermediates.

目次

Part I. Introduction. Polyamines: An Introduction, D. Morgan. Part II. Assay Methods for Enzymes of Polyamine Biosynthesis. Determination of Ornithine Decarboxylase Activity Using [3H]Ornithine, A. Tabib. Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine, C. Coleman and A. Pegg. Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity, L. Shantz and A. Pegg. Assay of Spermidine and Spermine Synthases, L. Wiest and A. Pegg. Measurement of Spermidine/Spermine N1-Acetyltransferase Activity, H. Wallace and D. Evans. Assay of Spermidine N8-Acetyltransferase, J. Blankenship. Part III. Assay Methods for Enzymes of Polyamine Catabolism. Assay of N8-Acetylspermidine Deacetylase, J. Blankenship. Hydrogen Peroxide Assay for Amine Oxidase Activity, R. Storer and A. Ferrante. Radiochemical Assay of Diamine Oxidase, R. Storer and A. Ferrante. Radiochemical Estimation of Polyamine Oxidase, D. Morgan. Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity, D. Morgan. Part IV. Measurement of Polyamines. Determination of Polyamines as Their Benzoylated Derivatives by HPLC, D. Morgan. A Dansyl Chloride-HPLC Method for the Determination of Polyamines, K. Hunter. The Determination of Polyamines and Amino Acids by a Fluorescamine-HPLC Method, K. Hunter and A. Fairlamb. Thin-Layer Chromatographic Method for Assaying Polyamines, R.Madhubala. Part V. Measurement of Polyamine Transport. Measurement of Polyamine Transport: Adherent Cells, D. Morgan. Measurement of Polyamine Transport: Cells in Suspension, S. Le Quesne and A. Fairlamb. Measurement of Polyamine Efflux from Cells in Culture, H. Wallace and A. Mackarel. Part VI. Measurement of Polyamine Effects on Cell Growth. Leucine Incorporation and Thymidine Incorporation, C. Denton. Tetrazolium (MTT) Assay for Cellular Viability and Activity, D. Morgan. Index.

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