The neuron in tissue culture

著者

    • Haynes, L. W. (Laurie W.)

書誌事項

The neuron in tissue culture

edited by L.W. Haynes

(IBRO handbook series : methods in the neurosciences, v. 18)

Wiley, c1999

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

During the 1990s, neural tissue culture has undergone a revolution. This volume in the IBRO Handbook Series contains 22 chapters from the range of fields to which neuronal tissue culture may be applied. Each method is well illustrated and includes exemplary data. Eight further chapters review environmental artefacts, the understanding and control of neuronal apoptosis and the substratum in neuronal culture. In addition, these later chapters provide many short protocols for the propagation of mammalian, avian, lower vertebrate and invertebrate nervous system tissues. Prominent treatment is given to organised culture formats for neurons, and the final chapter presents protocols for reconstucted and organotypic culture methods. This text also has step-by-step protocols needed to undertake cell culture in neuroscience, plus detailed applications of these methods.

目次

  • KEYNOTE CHAPTERS
  • The Neural Crest Colony Assay: Assessing Molecular Influences on Development in Culture (M. Sieber-Blum)
  • In Vitro Analysis of Neuronal Progenitor Cells from Mouse Olfactory Epithelium (A. Calof, et al.)
  • Analysis of Lineage-Dependent Phenotype Using Retroviral Transduction Techniques in Neural Progenitor Cells (G. Foster & B. Stringer)
  • In Vitro Analysis of Signal Mechanisms Involved in Neuronal Migration (H. Komuro & P. Rakic)
  • Regeneration of Adult Dorsal Root Ganglion Axons in Explant Culture (M. Kasper, et al.)
  • Calcium Homeostasis and Mechanisms of Growth Cone Formation During Neuritogenesis in Cultured Aplysia Neurons (M. Spira, et al.)
  • Mechanical Stimulation of Neurite Assembly in Cultured Neurons (S. Heidemann, et al.)
  • Modulation of Functional Synaptogenesis In Vitro Studied Using Two-Compartment Culture Chambers (P. Nelson, et al.)
  • Morphological and Functional Properties of Embryonic Rat Brainstem Motor Neurons in Organotypic Co-Culture with Tongue Muscle (J. Gueritaud & N. Ferrand)
  • Modulation of Single Developing Synapses Between Neurons and Myocytes of Xenopus Embryos In Vitro (X.-H. Wang & M.-M. Poo)
  • Analysis of Enteric Neural Circuits in Organ Culture of Adult Guinea-Pig (Z.-M. Song, et al.)
  • Role of Cajal-Retzius Cells in the Formation of Neuronal Connections in Entorhinohippocampal Organotypic Slice Cultures (J. Del Rio, et al.)
  • Establishment of Kitten Retinogeniculate Connections in Organotypic Co-Cultures (W. Guido & R. Erzurumlu)
  • Neuroanatomical and Physiological Changes of Avian Forebrain Regions Involved in Acoustic Imprinting in Roller Tube Co-Culture (H. Endepols and K. Braum)
  • High Spatial Resolution Imaging of the Excitation of Single Neurons in Culture (S. Tyc-Dumont & P. Gogan)
  • Interactions Between Peripheral Nerve Axons and Schwann Cells in Myelin-Forming Co-Cultures: Dynamics and Sodium Channel Distribution (S. Marshall & K. Angelides)
  • Fast Imaging of Subcellular Calcium Transients in Dendritic Spines of Cultured Hippocampal Neurons (M. Segal, et al.)
  • Physiology of Mature Crustacean Neurosecretory Neurons in Culture (I. Cooke)
  • Physiology of Identified Neurons from the Cockroach Meso- and Metathoracic Ganglia (E. Howes & P. Smith)
  • Viral Infection Studies in Adult Sensory Neurons (J. Castellanos & H. Hurtado)
  • Use of Spheroid Reaggregate Cultures for In Vitro Study of Brain Pathogenesis (R. Fox & C. Atterwill)
  • Brain Neuropathology in Co-Cultures of Central Nervous System Tissue with Monocytes (K. Dzenko, et al.)
  • PROTOCOLS FOR TISSUE AND CELL CULTURE
  • Nerve Cell Culture Methodology: The Medium Environment (G. Rebel, et al.)
  • Neuronal Apoptosis (M. Mattson, et al.)
  • Use of ECM Proteins to Optimize Neuronal Cell Function In Vitro (A. Goldberger & P. Flaherty)
  • Cell Culture of Neurons of the Peripheral Nervous System of Birds and Mammals (M. Welch, et al.)
  • Cell Culture of Neurons of the Fetal, Neonatal and Adult Central Nervous System of Birds and Mammals (V. Wong, et al.)
  • ...

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