Gene targeting : a practical approach
著者
書誌事項
Gene targeting : a practical approach
（The practical approach series, 212）
Oxford University Press, 1999, c2000
2nd ed
- pbk.
- hbk.
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Since the publication of the first edition of this work in 1993, there have been many advances in gene targeting. This new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition, the book concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre- lox P and Flp-FRT systems to produce "clean" germline mutations and conditionally (in)activating genes.
目次
- 1: Bradley et al: Gene targeting vectors for mammalian cells
- 2: Dymecki: Site specific recombination in cell and mice
- 3: Matise and Joyner: Production of targeted ES cell clones
- 4: Papaioannou and Johnson: Production of chimeras and genetically defined offspring from targeted ES cells
- 5: Nagy and Rossant: Production of completely ES cell-derived fetuses
- 6: Wurst and Gossler: Gene and enhancer traps in ES cells
- 7: Bultman and Magnuson: Mouse genetics and analysis of mutant phenotypes.
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