書誌事項

The ELISA guidebook

John R. Crowther

(Methods in molecular biology / John M. Walker, series editor, v. 149)

Humana Press, c2001

  • :hbk.

大学図書館所蔵 件 / 19

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

ISBN 9780896037281

内容説明

John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

目次

Overview of ELISA in Relation to Other Disciplines Systems in ELISA Stages in ELISA Titration of Reagents Theoretical Considerations Practical Exercises Monoclonal Antibodies Validation of Diagnostic Tests for Infectious Diseases Charting Methods for Internal Quality Control Immunochemical Techniques Test Questions
巻冊次

:hbk. ISBN 9780896039506

内容説明

John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

目次

Overview of ELISA in Relation to Other Disciplines. Systems in ELISA. Stages in ELISA. Titration of Reagents. Theoretical Considerations. Practical Exercises. Monoclonal Antibodies. Validation of Diagnostic Tests for Infectious Diseases. Charting Methods for Internal Quality Control. Immunochemical Techniques. Test Questions.

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