Hematopoietic stem cell protocols
著者
書誌事項
Hematopoietic stem cell protocols
（Methods in molecular medicine / John M. Walker, series editor, 63）
Humana , Blackwell Science, c2002
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内容説明・目次
内容説明
A group of leading stem-cell investigators describe in detail the key laboratory methods for investigating hematopoietic stem cells. Varied in scope, and richly detailed with step-by-step instructions and notes on the pitfalls to be avoided, these readily reproducible protocols apply hematopoietic stem cells isolated from mouse and human tissues of both embryonic and adult origin. The many approaches described employ not only molecular and cell biology techniques, but also animal model systems, and include hematopoietic stem cell expansion protocols and methods for the purification and genetic modification of stem cells. Comprehensive and state-of-the-art, Hematopoietic Stem Cell Protocols offers both novice and experienced investigators alike an invaluable compendium of highly practical techniques for studying a wide variety of stem cell topics, including blood formation, blood-based disease, and blood cancers such as leukemia.
目次
Non-invasive measurement of hematopoietic stem cell cycling and turnover by in vivo bromodeoxyuridine incorporation Isolation and characterizationof primitive hematopoietic cells based on their position in cell cycle Isolation and analysis of hematopoietic stem cells from mouse embryos The purification of mouse hematopoietic stem cells at sequential stages of maturation Method for purification of human hematopoietic stem cells by flow cytometry Flow cytometry and immunoselection of human stem cells Hematopoietic colony-forming cells The cobblestone area forming assay CFU-S: an assay for pluripotent myelopoietic stem cells Intrathymic injection for analysis of t cell progenitor activity Long-term culture-initiating cell assays for human and murine cells Quantitation of murine and human hematopoietic cells by limiting-dilution analysis in competitively repopulated hosts Ex vivo expansion of human and murine hematopoietic stem cells Genetic modification of murine hematopoietic stem cells by retroviruses Retroviral transduction of facs-purified hematopoietic stem cells Retroviral-mediated transduction and clonal integration analysis of human hematopoietic stem and progenitor cells Production of lentiviral vector supernatants and transduction of cellular targets Reverse transcriptase-pcr analysis of gene expression in hsc Identification of differentially expressed genes in sorted cell populations by two-dimensional gene expression fingerprinting Construction of cDNA libraries from purified populations of hematopoietic stem cells Hematopoietic development of ES cells in culture
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