Microbiology laboratory manual : principles and applications
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Microbiology laboratory manual : principles and applications
Prentice-Hall, 1997
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内容説明・目次
内容説明
This is a general introduction to the microbiology laboratory, including basic procedures and equipment. It contains 36 exercises which are divided into nine sections: microscopic technique, microbial diversity, microbial cultivation techniques, identification techniques, microbial growth, microbial control, clinical microbiology, virology and applied microbiology. The book is also provided with questions to reinforce understanding of the basic concepts, and to require students to analyze or apply the material under discussion.
目次
I. MICROSCOPIC TECHNIQUE.
1. Introduction to Microscopy.
2. Preparation of Smears, Simple Stains, and Wet Mounts
3. Differential Staining Procedures: Gram Stain, Acid- Fast Stain, Metachromatic-Granule Stain.
4. Additional Differential Staining Procedures: Demonstration of Bacterial Cytology.
5. Calibration of the Microscope.
6. Cell Dimensions: Measurement of Cell Size.
II. MICROBIAL DIVERSITY.
7. Survey of Microorganisms.
III. MICROBIAL CULTIVATION TECHNIQUES.
8. Isolation Techniques.
9. Oxygen Requirements of Microorganisms.
10. Physical Growth Requirements.
11. Selective and Differential Media.
12. Physiological Characteristics of Bacteria: Carbohydrate Metabolism.
13. Physiologic Characteristics of Bacteria: Reactions of Nitrogen Metabolism.
14. Physiologic Characteristics of Bacteria: Miscellaneous Reactions.
IV. IDENTIFICATION TECHNIQUES.
15. Development and Use of Diagnostic Keys.
16. Use of Diagnostic Keys: Bacterial Unknowns.
17. Rapid Diagnostic Tests: Profile Analysis.
V. MICROBIAL GROWTH.
18. Microbial Population Counts: Microscopic Counting Methods.
19. Microbial Population Counts: Viable Cell Counts.
20. Spectrophotometric Methods.
VI. MICROBIAL CONTROL.
21. Physical Control Methods.
22. UV Light as an Antimicrobial Agent.
23. Assay of Antimicrobial Agents: Disc-Diffusion Methods.
24. Assay of Antimicrobial Agents: Use-Dilution Methods.
25. Antibiotic Sensitivity Testing: The Kirby-Bauer Procedure.
26. Detection of Mutant Strains of Bacteria.
VII. CLINICAL MICROBIOLOGY.
27. Epidemiology.
28. Serological Reactions.
29. The Collection and Transport of Clinical Specimen.
30. Throat Cultures.
31. Urinary Tract Cultures.
32. Gastrointestinal Tract Cultures.
33. Lactobacillus Activity in Saliva.
VIII. VIROLOGY.
34. Viral Population Counts: Plaque Counting and Plaque Morphology.
IX. APPLIED MICROBIOLOGY.
35. Food Microbiology.
36. Water Analysis: Standard Methods and Membrane Filter Techniques.
Appendix A: Cultures Used.
Appendix B: Media, Stains, Reagents, and Supplies.
Appendix C: References.
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