Comprehensive enzyme kinetics
著者
書誌事項
Comprehensive enzyme kinetics
Kluwer Academic/Plenum Pub., c2003
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad.
目次
1. Introduction. 2. Chemical kinetics. 3. Kinetics of monosubstrate reactions. 4. Derivations of rate equations. 5. Linear inhibition. 6. Hyperbolic and parabolic inhibition. 7. Enzyme activation. 8. Kinetics of rapid equilibrium bisubstrate reactions. 9. Steady state kinetics of bisubstrate reactions. 10. Kinetic analysis of bisubstrate mechanisms. 11. Substrate inhibition and mixed dead-end and product inhibition. 12. Kinetics of trisubstrate reactions. 13. Cooperative and allosteric effects. 14. The pH dependence of enzyme catalysis. 15. Effects of temperature on enzyme reactions. 16. Isotope exchange. 17. Solvent and kinetic isotope effects. 18. Statistical analysis of initial rate and binding data. Subject index.
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