Library construction, physical mapping, and sequencing
Author(s)
Bibliographic Information
Library construction, physical mapping, and sequencing
（Methods in molecular biology / John M. Walker, series editor, v. 255 . Bacterial artificial chromosomes ; v. 1）
Humana Press, c2004
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
For both volumes:
Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.
Table of Contents
BAC Library Construction
Kazutoyo Osoegawa and Pieter J. de Jong
Construction of Small Genome BAC Library for Functional and Genomic Applications
David Smajs, Steven J. Norris, and George M. Weinstock
Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging
Sangita Pal, Solida Mak, and George M. Weinstock
Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions
Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine, Sun-Hee Leem, and Vladimir Larionov
Purification of BAC DNA
Tim S. Poulsen
Hybridization-Based Selection of BAC Clones
Chang-Su Lim and Ung-Jin Kim
Applications of Interspersed Repeat Sequence Polymerase Chain Reaction
Heike Zimdahl, Claudia Goesele, Thomas Kreitler, and Margit Knoblauch
BAC Mapping Using Fluorescence In Situ Hybridization
Xiao-Ning Chen and Julie R. Korenberg
High-Throughput BAC Fingerprinting
Jacqueline Schein, Tamara Kucaba, Mandeep Sekhon, Duane Smailus, Robert Waterston, and Marco Marra
BAC End Sequencing
Tim S. Poulsen and Hans E. Johnsen
Radiation Hybrid Mapping With BAC Ends
Michael Olivier, Shannon Brady, and David R. Cox
Shotgun Library Construction for DNA Sequencing
Bruce A. Roe
Rolling Circle Amplification for Sequencing Templates
Paul F. Predki, Chris Elkin, Hitesh Kapur, Jamie Jett, Susan Lucas, Tijana Glavina, and Trevor Hawkins
Transposon-Mediated Sequencing
Rachel Reeg and Anup Madan
Pyrosequencing: A Tool for DNA Sequencing Analysis
Elahe Elahi and Mostafa Ronaghi
Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions
Andrei Malykh, Nikolai Polushin, Alexei Slesarev, and Sergei Kozyavkin
Retrofitting BACs and PACs With LoxP Transposonsto Generate Nested Deletions
Pradeep K. Chatterjee
Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers
Pradeep K. Chatterjee and Joseph C. Baker, Jr.
BAC Finishing Strategies
Christine Bird and Darren Grafham
Using the TIGR Assembler in Shotgun Sequencing Projects
Mihai Pop and Dan Kosack
Finishing 'Working Draft' BAC Projects by Directed Sequencing With ThermoFidelase and Fimers
Andrei Malykh, Olga Malykh, Nikolai Polushin, Sergei Kozyavkin, and Alexei Slesarev
Optimized Multiplex Polymerase Chain Reaction: An Effective Method for Rapid Gap Closure
Diana Radune and Herve Tettelin
Assembly of DNA Sequencing Data
Jeremy Schmutz, Jane Grimwood, and Richard M. Myers
Sequence Finishing
Jeremy Schmutz, Jane Grimwood, and Richard M. Myers
Quality Assessment of Finished BAC Sequences
Jeremy Schmutz, Jane Grimwood, and Richard M. Myers
Index
by "Nielsen BookData"