Embryonic stem cells

Author(s)

• Klimanskaya, Irina
• Lanza, Robert

Bibliographic Information

Embryonic stem cells

edited by Irina Klimanskaya, Robert Lanza

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 418）

Academic Press, c2006

Note

Includes bibliographical references and indexes

Description and Table of Contents

Description

This is the first of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook" and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional" and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.

Table of Contents

SECTION I. DERIVATION AND MAINTENANCE OF EMBRYONIC STEM CELLS Murine embryonic stem cells. Bovine embryonic Stem Cells. Avian embryonic stem cells. Zebrafish embryonic stem cells. Human embryonic stem cells. Embryonic stem cells from morula. Embryonic stem cells from single-blastomeres. Embryonic stem cells from parthenotes. Embryonic stem cells using nuclear transfer. SECTION II. DIFFERENTIATION OF EMBRYONIC STEM CELLS Neurons and glia. Retinal pigment epithelium. Mesenchimal cells. Hematopoietic cells. Hematopoietic cells from primate ES cells. Vascular cells. Cardiomyocytes. Oocytes. Male germ cells. Insulin-producing cells. Pulmonary epithelium.

by "Nielsen BookData"

Details

• NCID: BA79383778
• ISBN:
  • 9780123736482
• Country Code: ne
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Amsterdam ; Tokyo
• Pages/Volumes: xl, 390 p., [20] p. of plates
• Size: 24 cm
• Subject Headings:
  • LCSH : Embryonic stem cells
  • LCSH : Cell differentiation
  • MESH : Embryonic Stem Cells
• Parent Bibliography ID:
  • BA00197543