Alzheimer's disease and frontotemporal dementia : methods and protocols
著者
書誌事項
Alzheimer's disease and frontotemporal dementia : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 670）
Humana Press, c2011
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Alzheimer's disease and frontotemporal dementia, two of the most prevalent neurodegenerative diseases, are dreaded disorders that attack the neural networks underlying memory and personality, systems that make us who we are. Major breakthroughs in recent years have spurred new research approaches to these conditions. In Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols, expert investigators bring together the many divergent areas of expertise used to study these diseases, including behavior, electrophysiology, confocal microscopy, and hardcore biochemistry. Beginning with an overview of the two diseases and contemporary research on them, the book continues with specialized protocols for working with amyloid- peptide, tau, and apolipoprotein E as well as experimental systems for studying AD and FTD, including cell and animal models, and outcome measures that can be used to assess neuronal function in these systems. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols serves as an ideal guide for all scientists pursuing the cause and cure of these widespread and nightmarishly debilitating disorders.
目次
1. Contemporary Approaches to Alzheimer's Disease and Frontotemporal Dementia
Erik D. Roberson
Part I: Working with AD- and FTD-Related Molecules
2. Preparing Synthetic A in Different Aggregation States
W. Blaine Stine, Lisa Jungbauer, Chunjiang Yu, and Mary Jo LaDu
3. Isolation of Low-n Amyloid -Protein Oligomers from Cultured Cells, CSF, and Brain
Ganesh M. Shankar, Alfred T. Welzel, Jessica M. McDonald, Dennis J. Selkoe, and Dominic M. Walsh
4. Detecting A *56 Oligomers in Brain Tissues
Mathew A. Sherman and Sylvain E. Lesne
5. Assessing A Aggregation State by Atomic Force Microscopy
Justin Legleiter
6. Measuring APP Carboxy-Terminal Fragments
Luke A. Esposito
7. Detection of APP Intracellular Domain (AICD) in Brain Tissue
Sanjay W. Pimplikar and Anupama Suryanarayana
8. Cell-Based Assays for Regulators of Tau Biology
Umesh K. Jinwal and Chad A. Dickey
9. Split GFP Complementation Assay for Quantitative Measurement of Tau Aggregation In situ
Wanjoo Chun, Geoffrey S. Waldo, and Gail V.W. Johnson
10. Apolipoprotein E Expression and Purification
Yvonne Newhouse and Karl H. Weisgraber
Part II: Model Systems and Outcome Measures
11. A Toxicity in Primary Cultured Neurons
Adriana Ferreira, Roxana C. Sinjoanu, Alexandra Nicholson, and Sara Kleinschmidt
12. Manipulation of Gene Expression in the Central Nervous System with Lentiviral Vectors
Binggui Sun and Li Gan
13. Selecting a Mouse Model of Alzheimer's Disease
Jeannie Chin
14. Monitoring Spatial Learning and Memory in Alzheimer's Disease Mouse Models Using the Morris Water Maze
Kimberly Scearce-Levie
15. Step-by-Step In Situ Hybridization Method for Localizing Gene Expression Changes in the Brain
Jorge J. Palop, Erik D. Roberson, and Inma Cobos
16. Real-Time Visualization of Axonal Transport in Neurons
Yasuko Osakada and Bianxiao Cui
17. Quantifying Biomarkers of Cognitive Dysfunction and Neuronal Network Hyperexcitability in Mouse Models of Alzheimer's Disease: Depletion of Calcium-Dependent Proteins and Inhibitory Hippocampal Remodeling
Jorge J. Palop, Lennart Mucke, and Erik D. Roberson
18. Epigenetic Changes in the Brain: Measuring Global Histone Modifications
Gavin Rumbaugh and Courtney A. Miller
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