Heme, chlorophyll, and bilins : methods and protocols
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Bibliographic Information
Heme, chlorophyll, and bilins : methods and protocols
Humana Press, c2010
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Includes bibliographical references and index
Description and Table of Contents
Description
In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators describe in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles,heme, and hemoproteins, to the biosynthesis and analysis of chlorophyll and bilins.
Table of Contents
Laboratory Methods for the Study of Tetrapyrroles
Alison G. Smith and Michael Witty
Syntheses of Tetrapyrroles
Kevin M. Smith
General Laboratory Methods for Tetrapyrroles
Jerry C. Bommer and Peter Hambright
Enzymatic Preparation of Tetrapyrrole Intermediates
Martin J. Warren and Peter M. Shoolingin-Jordan
Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme
Chang Kee Lim
Analysis of Intermediates and End Products of the Chlorophyll Biosynthetic Pathway
Constantin A. Rebeiz
Analysis of Heme and Hemoproteins
Angela Wilks
Hemoproteins Purification and Characterization by Using Aqueous Two-Phase Systems
Daniel Forciniti
Structural Study of Heme Proteins by Electron Microscopy of 2-Dimensional Crystals
Terrence G. Frey
Analysis and Reconstitution of Chlorophyll-Proteins
Harald Paulsen and Volkmar H.R. Schmid
Two-Dimensional Crystallization of Chlorophyll-Proteins
Georgios Tsiotis
Biosynthesis and Analysis of Bilins
Matthew J. Terry
Analysis and Reconstitution of Phytochromes
Michael T. McDowell and J. Clark Lagarias
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment Reactions
Wendy M. Schluchter and Donald A. Bryant
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