Search Results 1-20 of 8335

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Efficient Reaction Deletion Algorithms for Redesign of Constraint-based Metabolic Networks for Metabolite Production with Weak Coupling

Tamura Takeyuki

… A recent method that ensures the target metabolite production even when the cell growth is not maximized (strong coupling) has been shown to be able to identify such computational redesign for nearly all metabolites included in the genome-scale metabolic models of Escherichia coli … and Saccharomyces cerevisiae …

IPSJ Transactions on Bioinformatics 14(0), 12-21, 2021

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2

Evaluation of C. perfringens cpe-positive Strain as a Source Tracking Indicator of Human Contamination in Freshwater Environments

Suzuki Hiroyuki , Oonaka Kenji , Hashimoto Atsushi

… In this study, a field survey of fecal contamination indicators and Clostridium perfringens cpe(+) in river environments was conducted to verify the effectiveness of C. … perfringens cpe(+) as a microbial source tracking indicator in public water bodies. …

Journal of Water and Environment Technology 19(1), 1-12, 2021

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3

Recombinant unpurified rETXH106P/CTB-rETXY196E protects rabbits against Clostridium perfringens epsilon toxin

PENG Xiaobing , LI Xuni , PENG Guorui , FENG Lifang , JIANG Yuwen , LUO Yufeng

… Epsilon toxin (ETX), produced by Clostridium perfringens … Rabbits were immunized twice with five inactivated recombinant … Escherichia coli (E. … coli) vaccines containing 100 μg/ml …

Journal of Veterinary Medical Science, 2021

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4

A partial photoreactivation defect phenotype is not due to unrepaired ultraviolet-induced pyrimidine dimers in ultraviolet-sensitive mutants of Neurospora crassa

Tsukada Kotaro , Yoshihara Ryouhei , Hatakeyama Shin , Ichiishi Akihiko , Tanaka Shuuitsu

… In the filamentous fungus … Neurospora crassa, some UV-sensitive mutants such as mus-44 … In this study, the N. … crassa … CPD photolyase PHR was overexpressed in the mus-44 …

Genes & Genetic Systems, 2021

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5

Direct and rapid detection of extended-spectrum β-lactamase by chromogenic Cica-β test using culture fluid of positive blood culture bottles [in Japanese]

TANAKA Makito , SHINAGAWA Masaaki , TAKAHASHI Satoshi

… 当院の血液培養検査にて検出されたEscherichia coli，Klebsiella pneumoniae，Klebsiella oxytocaおよびProteus mirabilisの4菌種68株を対象とした。 …

Japanese Journal of Medical Technology 70(1), 128-131, 2021

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Two cases of emphysematous cystitis diagnosed by ultrasonography [in Japanese]

NAGASAKI Masaharu , MATSUOKA Keiko , INOUE Mami , NAGAYAMA Nobuko

… 尿培養にて2例ともにEscherichia coliが分離され，薬剤感受性試験の結果，extended-spectrum β-lactamase（ESBL）産生菌と判定された。 …

Japanese Journal of Medical Technology 70(1), 172-175, 2021

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7

High prevalence of a gene cluster conferring resistance to streptomycin, sulfonamide, and tetracycline in Escherichia coli isolated from indigenous wild birds

Lee Hye-Jin , Woo Yong-Ku , Choi Byung-Kook , Jeong Ok-Mi , Kim Jin-Hyun , Kim Dong-Wan , Jeong Ji-Yeon , Kwon Yong-Kuk , Kang Min-Su

… A total of 116 Escherichia coli … Seventy-one isolates from sparrows (Passer montanus) and one isolate from doves (Columba livia) were resistant to three antimicrobials, including streptomycin, sulfonamide, and tetracycline (SSuT). …

The Journal of General and Applied Microbiology, 2021

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8

Antimicrobial resistance in Escherichia coli isolated from brown rats and house shrews in markets, Bogor, Indonesia

LE HUY Hoang , KOIZUMI Nobuo , NURADJI Harimurti , Susanti , NOOR Susan M , Indi Dharmayanti NLP , HAGA Takeshi , HIRAYAMA Kazuhiro , MIURA Kozue

… We captured 87 small mammals (79 brown rats and 8 house shrews) in four markets, Bogor, Indonesia in October 2019, and we obtained 20 AMR Escherichia coli … Of these, eight isolates were determined to be multi-drug resistant (MDR) E. … coli. … This study shows that AMR E. …

Journal of Veterinary Medical Science, 2021

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9

Multidisciplinary Treatment for Vertebral Osteomyelitis at our Hospital: An 11-Year Review of 117 Cases [in Japanese]

Mori Gen , Inoue Atsuo , Okumura Hisashi , Ueda Hideki , Ikeda Takumi , Osawa Toru , Takeura Nobuaki , Idei Chihiro , Sukenari Tsuyoshi , Oishi Hisao , Matoba Hiroe , Yoshihara Yasushi , Kuribayashi Masaaki

はじめに：化膿性脊椎炎は，発症年齢が高齢化し，発症数も増加している．当院では，抗菌薬の選択を感染制御部の，CTガイド下生検およびドレナージを放射線科の協力体制のもとに治療を行っている．本研究では，本症の臨床的背景と治療経過について調査した．対象と方法：対象は11年間で …

Journal of Spine Research 12(1.2), 4-9, 2021

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10

Protein engineering of the soluble metal-dependent formate dehydrogenase from Escherichia coli

Fuji Rintaro , Umezawa Koji , Mizuguchi Manami , Ihara Masaki

Analytical Sciences, 2021

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11

Association between antimicrobial treatment and resistance of pathogenic Escherichia coli isolated from diseased swine in Kagoshima Prefecture, Japan

MISUMI Wakako , FUNAMORI Taruho , HAMADA Kyohei , IWAMOTO Jiro , FUJISONO Shoichiro , CHITOSE Kenichi , KUSUMOTO Masahiro

… Pathogenic Escherichia coli … In the present study, we determined the O serogroups, virulence factors, and antimicrobial resistance of 360 E. … coli … The isolates of the predominant serogroups O139, OSB9, O149, O8, and O116 possessed virulence factor genes typically found in diarrheagenic E. …

Journal of Veterinary Medical Science, 2021

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12

The importance of genetic research on the dominant species of human intestinal indigenous microbiota

KURIHARA Shin

… Comparison of the results with those obtained by in silico … Next, strains with gene deletions and complementation for the polyamine synthetic system of the genus Bacteroides … Furthermore, we co-cultured genetically engineered Escherichia coli …

Bioscience of Microbiota, Food and Health 40(1), 19-26, 2021

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13

Expression, Purification and Characterization of CAR/NCOA-1 Tethered Protein in E. coli Using Maltose-Binding Protein Fusion Tag and Gelatinized Corn Starch

Kobashigawa Yoshihiro , Morioka Hiroshi , Namikawa Mana , Sekiguchi Mitsuhiro , Inada Yuki , Yamauchi Soichiro , Kimoto Yuu , Okazaki Kyo , Toyota Yuya , Sato Takashi

… We prepared recombinant CAR protein using the Escherichia coli …

Biological and Pharmaceutical Bulletin 44(1), 125-130, 2021

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14

Reduction in Bacterial Isolation Rate with Improved Compliance for the Use of Alcohol-based Hand Rub [in Japanese]

MURATA Akiko , KAWASHIMA Kaoru , HATAKEYAMA Eiji , YAMAZAKI Ayaka , MATSUMOTO Katsuki , FUKASAWA Takashi , MATSUMOTO Natsuki , KAMIYA Akane , FUJITA Hiroyuki

… 擦式アルコール手指消毒薬の消費量が医療関連感染との関連性が深いメチシリン耐性黄色ブドウ球菌（MRSA），Serratia marcescens，基質特異性拡張型βラクタマーゼ（ESBL）産生大腸菌の検出率と関連がみられるかを後方視的に検討した．擦式アルコール手指消毒薬使用量を増加させるために，2014年より病棟ラウンドで個々の看護師に対して手指衛生の手法に関する直接指導を行い， …

Japanese Journal of Infection Prevention and Control 35(4), 163-167, 2020

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15

Importance of Candidate Phyla Radiation (CPR) Bacteria for the Origin of Life [in Japanese]

TSURUMAKI Megumi , SAITO Motofumi , MARUYAMA Shigenori , KANAI Akio

… Thus, their genomes are remarkably small compared to other well-known and ordinary bacteria, represented by Escherichia coli, which have over 4,000 genes, but are similar to those of symbiotic or parasitic bacteria. …

Journal of Geography (Chigaku Zasshi) 129(6), 881-898, 2020

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16

Application of Lactic Acid Bacteria for Enhanced Food Safety of Cambodian Fermented Small Fish (Pha-ork Kontrey)

Chrun Rithy , Born Pichpunleu , Huon Thavrak , Buntong Borarin , Chay Chim , Inatsu Yasuhiro

… "Pha-ork Kontrey" is a Cambodian traditional fermented small fish and is characterized by a low salt concentration and short fermentation period. … Five log CFU/g of Escherichia coli … or Staphylococcus aureus …

Food Science and Technology Research 26(6), 687-694, 2020

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17

Enhancement of antibacterial property of titanium by two-step micro arc oxidation treatment

SHIMABUKURO Masaya , TSUTSUMI Harumi , TSUTSUMI Yusuke , MANAKA Tomoyo , CHEN Peng , ASHIDA Maki , ISHIKAWA Kunio , KATAYAMA Hideki , HANAWA Takao

… The MAO-treated specimens containing both Ag and Zn showed excellent antibacterial properties against Escherichia coli, and the properties were sustained even after 28 days of immersion in physiological saline to simulate the living environment. …

Dental Materials Journal, 2020

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18

Evaluation of Microorganisms and Surface Damage of Wooden and Plastic Cutting Boards [in Japanese]

TAKAO Tetsuya , FUJIMOTO Kiyohiko , NAKAYAMA Eiko , SASAKI Nakaba , NAGAI Hiroshi , MOMOHARA Ikuo

　木製まな板およびエンボス加工されたプラスチック製まな板について, 新品および使用済みのまな板をそれぞれ3枚入手し, 模擬的な調理後の洗浄, 次亜塩素酸消毒処理, 自然乾燥の各工程における一般生菌数および大腸菌群数を測定した. また, まな板の表面粗さの定量的な検討を行い, 粗さと生菌数等との関係を検討した. 模擬的な調理を行った後の, 洗浄, 消毒, 乾燥工程後の一般生菌数及び …

Journal of Home Economics of Japan 71(12), 757-765, 2020

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19

Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan

SATO Wataru , SUEYOSHI Masuo , SUKMAWINATA Eddy , UEMURA Ryoko , KANDA Takuya , KUSANO Kanichi , KAMBAYASHI Yoshinori , SATO Takashi , ISHIKAWA Yuhiro , TOYA Ryohei

… In this study, we investigated the occurrence of antimicrobial resistance in commensal Escherichia coli isolated from healthy Thoroughbred (TB) racehorses in Japan. … coli was isolated by using selective agar media, deoxycholate-hydrogen sulfide-lactose (DHL) and eosin methylene blue (EMB). … coli isolates were examined against 10 antimicrobial agents by using the broth microdilution method. …

Journal of Equine Science 31(4), 85-91, 2020

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20

Removal of Microbes from Raw Sewage by Stepwise Advanced Treatment [in Japanese]

TAKAHASHI YOSHIHITO , OGIHARA JUN

 二次処理水中のBOD、全窒素（TN）、および全リン（TP）を削減する段階的高度処理法（実験系）と標準活性汚泥法（対照系）の両方を14か月間比較した。 処理性能はスチューデントのt検定により評価した。その結果、実験系のBOD、TN、TPは段階的高度処理の目標水質を満たし、対照系と比べて大幅に低減した。次に、衛生微生物（大 …

Japanese Journal of Water Treatment Biology 56(4), 79-90, 2020

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