CiNii Articles - 遺伝子導入

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7. 共同利用研究, 7.2.研究成果, 7.2.1.計画研究 [in Japanese]

2019-A-15 Conservation genetics of Myanmar's macaques: a phylogeographical approach2019-A-19 Analysis of mitochondrial sequences for species identification and evolutionary study of slow loris (genus …

霊長類研究所年報 = Annual Reports of the Primate Research Institute Kyoto University (50), 94-102, 2020-10-01

IR
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Novel in utero gene transfer system for embryos reveals mechanisms for the onset of neurogenesis [in Japanese]

岸雄介 , 桑山尚大

細胞 52(9), 519-523, 2020-08

Ichushi Web
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Optogenetic gene therapy [in Japanese]

菅野江里子 , 冨田浩史

眼科 62(7), 669-675, 2020-07

Ichushi Web
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〈報告〉哺乳動物細胞における可溶型リンフォトキシン受容体タンパク質の発現 [in Japanese]

早坂晴子

… LTβR-Ig 遺伝子発現プラスミドをHEK293T 細胞に遺伝子導入し、3種類の異なるタンパク質発現用無血清培地 NeoPro-293A、HE400、HE200 で培養し、LTβR-Igタンパク質の回収率を比較した。 …

理工学総合研究所研究報告 = Annual reports by Research Institute for Science and Technology (31), 55-61, 2020-02-28

IR
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Establishment of Induced Pluripotent Stem Cells from Dedifferentiated Adipocytes [in Japanese]

Hashimoto Makoto , Uekusa Shota , Ono Kako , Goto Shumpei , Hosokawa Takashi , Koshinaga Tsugumichi , Matsumoto Taro

Dedifferentiated fat cells (DFATs) are multipotent cells, similar to mesenchymal stem cells obtained with theceiling culture method for mature adipocytes. In this study, we tried to generate induced p …

Journal of Nihon University Medical Association 79(5), 275-286, 2020

J-STAGE
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Research on Microfluidic Chip for High-throughput Gene Transfer into Cells by Electrically-induced Bubbles [in Japanese]

Michimoto Taiga , Uning Kevin Theodric , Ichikawa Keita , Yamanishi Yoko

In this study, we made a device that can perform cell manipulation of oocytes and reagent injection to a single cell. This two-dimensional chip composed of two functions which is; (1) loading …

The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec) 2020(0), 1A1-N13, 2020

J-STAGE
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New Development of Ultrasonic Cancer Therapy:- The Possibility and Some of the Difficulties -

KONDO TAKASHI , SUZUKI RYO , SASAKI NOBORU , TAKIGUCHI MITSUYOSHI

Biophysical modes of ultrasound are classified as follows: 1) thermal effects, 2) cavitational effects, 3) the other nonthermal and noncavitational effects. There were three approaches to can …

Thermal Medicine 36(3), 75-90, 2020

J-STAGE Ichushi Web
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Advances in gene therapy and genome editing therapy for hemophilia [in Japanese]

Kashiwakura Yuji , Ohmori Tsukasa

Hemophilia is an X-linked inherited bleeding disorder resulting from defects in the gene F8 (hemophilia A) or F9 (hemophilia B). Prophylaxis administrati …

The Japanese Journal of Pediatric Hematology / Oncology 57(3), 220-226, 2020

J-STAGE
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Effect of culture conditions for maintaining undifferentiated state of bovine iPS cells [in Japanese]

KITA Yuto , HIRAIDE Misuzu , NISHIMURA Yuka , KOBAYASHI Msayuki

… 【背景・目的】マウス体細胞に山中4因子（Oct4，Sox2，Klf4，c-Myc）を遺伝子導入することにより，マウスiPS細胞が樹立された。 …

The Journal of Reproduction and Development Supplement 113(0), P-103-P-103, 2020

J-STAGE
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Generation of GATA4-flox mouse by gene targeting method [in Japanese]

IWASAKI Tsugumi , MURATA Kousuke , KAWAMURA Meiko , NAKATSUKASA Ena , ABE Manabu , NATSUME Rie , SUGIMURA Satoshi , SAKIMURA Kenji , YAMASHIRO Hideaki

… 【材料・方法】C57/BL6ゲノムライブラリーよりBACクローンを単離，DNAを抽出し，Red/ET組み換え法によるBACサブクローニング法と多重遺伝子導入法を用いてターゲッティングベクターを作製した。 …

The Journal of Reproduction and Development Supplement 113(0), P-11-P-11, 2020

J-STAGE
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Transduction of the TXNIP gene into AML cell lines by lentiviral vectors [in Japanese]

Noura Mina , Masutani Hiroshi

… 本研究ではAML におけるTXNIP の機能解析を目的とし，レンチウイルスベクターによるAML 細胞株への遺伝子導入を行った。 …

Bulletin of Tenri Health Care University 8(1), 47-51, 2020

J-STAGE
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Nonclinical studies on CRS risk with cancer immunotherapy [in Japanese]

MISHIMA Masayuki

Immune checkpoint inhibitors, bispecific T-cell engagers and CAR-T therapy have provided unique therapeutic effects. Currently, great efforts are underway to develop various therapeutics of t …

Annual Meeting of the Japanese Society of Toxicology 47.1(0), S3-1, 2020

J-STAGE
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Contribution of reporter gene transgenic rodents to toxicology [in Japanese]

UMEMURA Takashi

Reporter gene mutation assay has been noted as an in vivo genotoxicity test considering ADME and target sites of test chemicals. However, this assay sometimes demonstrates incompatible result …

Annual Meeting of the Japanese Society of Toxicology 47.1(0), S31-1, 2020

J-STAGE
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Preclinical safety evaluation of cancer immunotherapy products at PMDA [in Japanese]

NONAKA Mizuho

Recently, various modalities of cancer immunotherapy products have been developed, such as antibody, peptide vaccine, gene therapy product which expresses the cancer antigen in vivo, and chim …

Annual Meeting of the Japanese Society of Toxicology 47.1(0), S3-2, 2020

J-STAGE
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Mutagenicity in germ cells and detection of de novo mutations in the offspring [in Japanese]

MASUMURA Kenichi

Gene mutation induced by environmental mutagens is an important health factor. Germline mutations are sources of genetic diversity but may cause adverse outcome such as genetic diseases. We i …

Annual Meeting of the Japanese Society of Toxicology 47.1(0), S31-2, 2020

J-STAGE
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Reproductive and developmental toxicity in a humanized-transgenic mouse with human placental endocrine function [in Japanese]

NAKANISHI Tsuyoshi

The estrogen level of rodent fetus is much lower than that of human fetus because the rodent placenta doesn't have aromatase which converts androgen to estrogen. To examine the species differ …

Annual Meeting of the Japanese Society of Toxicology 47.1(0), S16-4, 2020

J-STAGE
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Results and challenges in subretinal injection in rats, rabbits, and cynomolgus monkeys [in Japanese]

SAKATA Megumi , KABAYAMA Koji , ARAKI Tomoaki , TSUSAKI Hideshi

Subretinal injection is widely used in gene therapy research on age-related macular degeneration and retinitis pigmentosa. Rats, rabbits, and cynomolgus monkeys are often used, but the maximu …

Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-48E, 2020

J-STAGE
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Involvement of immune tolerance systems in the onset of HLA allele-specific drug-induced immunotoxicity [in Japanese]

AOKI Shigeki , KUWAHARA Saki , SUSUKIDA Takeshi , ITO Kousei

[Aim] We generated a transgenic mouse carrying HLA-B*57:01 to reproduce abacavir-induced idiosyncratic toxicity. Here, to observe clear toxic phenotype, we eliminated immunosuppressive factor …

Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-61E, 2020

J-STAGE
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Investigation of HLA allotype-specific skin toxicity by abacavir using HLA transgenic mice [in Japanese]

YAMADA Yushiro , AOKI Shigeki , FUJIMORI Sota , ITO Kousei

[Aim] We investigated drug reactions in keratinocytes (KCs) by focusing on the interaction between HLA-B*57:01 and abacavir (ABC) using our originally produced HLA-B*57:01 transgenic mice (B* …

Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-62S, 2020

J-STAGE
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Development of Microdroplet-Based Electroporation System for Designing Cellular Functions (6th Report):Evaluation of Probability of Cell Membrane Perforation and Cell Viability [in Japanese]

Ishii-Teshima Miho , Binti Azhar Siti Syuhada , Kurita Hirofumi , Numano Rika , Nagai Moeto , Shibata Takayuki

… 本研究では高効率かつ低侵襲な遺伝子導入技術の実現を目指し、マイクロ流路を用いて微小液滴内に細胞と導入遺伝子を封入し、連続的に電気穿孔を行う手法の開発を行っている。 …

Proceedings of JSPE Semestrial Meeting 2020S(0), 148-149, 2020

J-STAGE

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