CiNii Articles - Blastocyst

1

Mitochondrial reactive oxygen species regulate mitochondrial biogenesis in porcine embryos

KAGEYAMA Mio , ITO Jun , SHIRASUNA Koumei , KUWAYAMA Takehito , IWATA Hisataka

… These embryos were cultured in media containing either 0.1 μM MitoTEMPOL (MitoT), 0.5 μM Mitoquinol (MitoQ), or vehicle (ethanol) for 5 days to determine the rate of development to the blastocyst stage. … One day of incubation with the antioxidants reduced the ROS content in the embryos but did not affect the rate of development to the blastocyst stage. …

Journal of Reproduction and Development, 2021

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2

Different response of embryos originating from control and obese mice to insulin in vitro

KŠIŇANOVÁ Martina , KOVAŘÍKOVÁ Veronika , ŠEFČÍKOVÁ Zuzana , ŠPIRKOVÁ Alexandra , ČIKOŠ Štefan , PISKO Jozef , FABIAN Dušan

… Two-cell and eight-cell embryos recovered from obese and control mice were cultured in a medium supplemented with 1 or 10 ng/ml insulin until blastocyst formation. …

Journal of Reproduction and Development 67(1), 25-34, 2021

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3

Lipid droplets are formed in 2-cell-like cells

FURUTA Asuka , NAKAMURA Toshinobu

… Embryonic stem (ES) cells, derived from the inner cell mass of a blastocyst, are believed to pluripotent cells and give rise to embryonic, but not extraembryonic, tissues. …

Journal of Reproduction and Development, 2021

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4

Three-dimensional live imaging of bovine embryos by optical coherence tomography

MASUDA Yasumitsu , HASEBE Ryo , KUROMI Yasushi , KOBAYASHI Masayoshi , IWAMOTO Misaki , HISHINUMA Mitsugu , OHBAYASHI Tetsuya , NISHIMURA Ryo

… fertilization that had reached the expanded blastocyst stage after 7 days of culture were transferred after imaged by OCT. Twenty-two parameters, including thickness and volumes of the inner cell mass, trophectoderm, and zona pellucida, and volumes of blastocoel and whole embryo, were quantified from 3D images. …

Journal of Reproduction and Development, 2021

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5

Functional kidney regeneration using pluripotent stem cells [in Japanese]

齊藤弥積 , 横尾隆

腎臓内科 = Nephrology 12(4), 418-425, 2020-10

Ichushi Web
6

An approach to kidney regeneration by blastocyst complementation [in Japanese]

渡邊將人 , 松成ひとみ , 長嶋比呂志

腎臓内科 = Nephrology 12(3), 289-295, 2020-09
7

Generation of functional lungs via conditional blastocyst complementation using pluripotent stem cells [in Japanese]

森宗昌

臨床免疫・アレルギー科 = Clinical immunology & allergology 73(6), 658-663, 2020-06

Ichushi Web
8

Generation of transplantable organs by blastocyst complementation [in Japanese]

長嶋比呂志 , 松成ひとみ

医学のあゆみ 273(9), 820-825, 2020-05-30

Ichushi Web
9

Usefulness of vitrification device having vitrification solution absorber for cryopreservation of mouse embryos at the blastocyst stage

Abe Shiori , Koyanagi Yumi , Nakano Marina , Sasaki Miku , Matsuzawa Atsushi , Yoshida Kakeru , Tokunaga Yukio , Momozawa Kenji , Miyake Takao

Japanese Journal of Veterinary Research 68(1), 63-68, 2020-02

IR DOI Ichushi Web
10

Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

KOBAYASHI Mitsuru , ITO Jun , SHIRASUNA Koumei , KUWAYAMA Takehito , IWATA Hisataka

… The rate of development to the blastocyst stage was higher for the D-group than for the H-group. … After activation, the mitochondrial content was constant before the blastocyst stage in D-group embryos, but increased earlier in H-group embryos. …

Journal of Reproduction and Development 66(6), 539-546, 2020

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11

Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

PENG Yan-xia , KIM Nam-Hyung , JIANG Hao , ZHANG Jia-Bao , CHEN Cheng-Zhen , LUO Dan , YU Wen-jie , LI Sheng-peng , XIAO Yue , YUAN Bao , LIANG Shuang , YAO Xue-rui

… culture medium to explore the influence of CA on apoptosis, proliferation, blastocyst formation, reactive oxygen species (ROS) levels, glutathione (GSH) levels, mitochondrial membrane potential, and embryonic development-related gene expression. … culture significantly improved the cleavage rates, blastocyst formation rates, hatching rates, and total numbers of cells of parthenogenetically activated porcine embryos compared with no supplementation. …

Journal of Reproduction and Development 66(6), 555-562, 2020

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12

Locus-specific analysis of DNA methylation patterns in cloned and in vitro fertilized porcine embryos

XU Weihua , LI Hongyi , ZHANG Mao , SHI Junsong , WANG Zhengchao

… DNA methylation at the blastocyst stage. …

Journal of Reproduction and Development 66(6), 505-514, 2020

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13

Analysis of cell polarity and YAP cellular localization toward elucidation on the mechanism of polar trophectoderm-deficient blastocyst by Brd4 inhibition [in Japanese]

MATSUMOTO Sachi , WATANABE Ren , MOCHIZUKI Kazuki , KISHIGAMI Satoshi

【目的】ブロモドメインタンパク質Brd4は初期胚から成体まで発現し，特に胚盤胞の内部細胞塊の正常な発生に不可欠である。我々は，IVF胚を2細胞期初期からBrd4阻害を行った場合，胚盤胞率が低下し，胚盤胞において栄養外胚葉の細胞数の減少や極栄養外胚葉欠落が高頻度で起こることを見出し報告した（第112回本大会）。本研究ではこの機構を解明するため，胚盤胞形成過程における細胞極性およびY …

The Journal of Reproduction and Development Supplement 113(0), P-52-P-52, 2020

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14

Peptidoglycan disrupts the signals of embryo-secreted interferon-tau in the bovine endometrium [in Japanese]

ZINNAH Mohammad Ali , SHIMADA Masayuki , IMAKAWA Kazuhiko , MIYAMOTO Akio , MAREY Mohamed Ali , AKHTAR Ihshan M , IBRAHIM Ibrahim F , MATSUNO Yuta , ELWEZA Ahmed E , MA Dongxue , FIORENZA Mariani , MOTOKI Sasaki

… Blastocyst-secreted interferon-tau (IFNT) induces upregulation of interferon-stimulated genes (ISGs) via …

The Journal of Reproduction and Development Supplement 113(0), P-113-P-113, 2020

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15

TLR2 pathway is involved in sperm acrosome reaction for penetration through zona pellucida for fertilization [in Japanese]

MA Dongxue , MAREY Mohamed Ali , FIORENZA Mariani Farias , MIYAMOTO Akio

… The cleavage rate and blastocyst ratio were assessed after 42 h and 7 days post-insemination respectively. … [Results]Co-culture of TLR1/2 antagonist-treated sperm with either COCs or cumulus-free oocytes significantly suppressed the cleavage rate and blastocyst ratio compared to control. …

The Journal of Reproduction and Development Supplement 113(0), P-43-P-43, 2020

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16

Effect of neurotensin on cultured mouse preimplantation embryos

HIRADATE Yuki , HARA Kenshiro , TANEMURA Kentaro

… Supplementation of 1, 10, 100 or 1000 nM NTS to embryo culture medium after fertilization showed that 100 nM NTS significantly improved the blastocyst formation. …

Journal of Reproduction and Development 66(5), 421-425, 2020

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17

Improvement of a twice collection method of mouse oocytes by surgical operation

INOUE Rei , HARADA Kana , WAKAYAMA Sayaka , OOGA Masatoshi , WAKAYAMA Teruhiko

… However, the blastocyst and offspring rates of those oocytes after IVF or ICSI were the same as those of the control regardless of the recovery day period. …

Journal of Reproduction and Development 66(5), 427-433, 2020

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18

Lipopolysaccharide-induced mechanisms of ovarian dysfunction in cows with uterine inflammatory diseases

MAGATA Fumie

… Moreover, oocytes matured using LPS treatment are less likely to develop into the blastocyst stage. …

Journal of Reproduction and Development 66(4), 311-317, 2020

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19

Ruthenium red improves blastocyst developmental competence by regulating mitochondrial Ca2+ and mitochondrial functions in fertilized porcine oocytes in vitro

JEGAL Ho-Geun , PARK Hyo-Jin , KIM Jin-Woo , YANG Seul-Gi , KIM Min-Ji , KOO Deog-Bon

… fertilization (IVF) on subsequent blastocyst developmental capacity in porcine is unclear. … levels using RR and/or histamine in fertilized oocytes and their influence on blastocyst developmental capacity in pigs. … Blastocyst development rate of 20 μM RR-treated zygotes was significantly increased 6 h after IVF (P < … Conversely, the blastocyst development rate was significantly decreased in histamine (mito-Ca2+ …

Journal of Reproduction and Development 66(4), 377-386, 2020

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20

Generation of the functional organ by utilizing in vivo developmental environment [in Japanese]

Yamaguchi Tomoyuki

再生医学における細胞治療の分野は目覚ましく発展し, in vitroで分化誘導した細胞が実際の治療に用いられようとしているが, 3次元構造をもった臓器はin vitroで作製することが困難である. これに対して, 胚盤胞補完法は多能性幹細胞のキメラ形成能を利用し, 動物体内で形態も機能も完全な臓器を作出す …

Organ Biology 27(2), 149-154, 2020

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