Search Results 1-20 of 72

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  • TAK-242, a specific inhibitor of Toll-like receptor 4 signalling, prevents endotoxemia-induced skeletal muscle wasting in mice

    Ono Yuko , Maejima Yuko , Saito Masafumi , Sakamoto Kazuho , Horita Shoichiro , Shimomura Kenju , Inoue Shigeaki , Kotani Joji

    … Here, we tested the effects of TAK-242, a Toll-like receptor 4 (TLR4)-specific signalling inhibitor, on myotube atrophy in vitro and muscle wasting in vivo induced by endotoxin. …

    Scientific Reports (10), 694, 2020-01-20

    IR 

  • Anti-diabetic effect of ethanol extract of <i>Cyclolepis genistoides</i> D. Don (Palo azul), made in Paraguay  [in Japanese]

    Sato Hiromi , Okawa Toya , Hisaka Akihiro , Ueno Koichi , Funaki Asami , Kimura Yuki , Sumitomo Mai , Yoshida Hiroya , Okumura Akiko , Fukata Hideki , Hosoyama Hiroshi , Kuroda Masayuki

    … 250 mg/kg投与群では,PPARγおよびその標的であるadiponectin mRNA発現の増加とともに,皮下脂肪重量を減少させる傾向が確認された.また,別のインスリン標的細胞として,筋肉分化への影響を調べた.C2C12マウス筋芽細胞においてPaloは,IGF-1,myogenin,ミオシン重鎖(MHC)などの筋分化マーカーの発現を濃度依存的に増加させ,また5'-AMP活性化プロテインキナーゼ(AMPK)の活性化を誘導した.さらに,分化誘導条 …

    Folia Pharmacologica Japonica 155(4), 202-208, 2020

    J-STAGE 

  • 3-Iodothyronamine-mediated Metabolic Suppression in in vivo and in vitro Models: Potential Application to Emergency Medicine and Long-duration Spaceflights

    JU Hyunwoo , SHIN Haksup , KIM Taewan , KIM Minkyu , PARK Junsoo , PARK Kyoungsook , CHOI Inho

    … In the in vitro studies, T1AM-treated C2C12 myotubes decreased the MR to 36%, and upregulated activation of AMP-activated protein kinase and acetyl-CoA carboxylase, but inhibited signaling activities associated with glycolytic activity. …

    International journal of microgravity science and application 37(1), 370102, 2020

    J-STAGE 

  • Morphology-Based Analysis of Myoblasts for Prediction of Myotube Formation

    Ishikawa Kiyoshi , Yoshida Kei , Kanie Kei , Omori Kenji , Kato Ryuji

    … In this study, we demonstrate that morphology-based cellular evaluation of unlabeled cells is an efficient approach to evaluate myotube formation assays. … One of our aims was to study the myogenic differentiation process in C2C12 cells to discern the differences between cellular responses to different medium conditions (serum concentrations and insulin dosages). …

    SLAS DISCOVERY: Advancing Life Sciences R&D 24(1), 47-56, 2019-01-01

    IR 

  • The role of calcineurin B homologous protein 3 (CHP3) in myoblast  [in Japanese]

    Kobayashi Soushi , Nobe Koji

    … In mouse myoblast cell line, C2C12, almost no endogenous CHP3 protein was expressed. … Immunofluorescent studies showed that CHP3 was co-localized with GSK3beta in the myotube. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 3-P-087, 2019

    J-STAGE 

  • Diosgenin Supplementation Prevents Lipid Accumulation and Induces Skeletal Muscle-Fiber Hypertrophy in Rats

    KUSANO Yuri , TSUJIHARA Nobuko , MASUI Hironori , SHIBATA Takahiro , UCHIDA Koji , TAKEUCHI Wakako

    … Mouse myoblast-derived C2C12 cells were used to examine whether Dio directly affected skeletal muscle. … Furthermore, in myotube C2C12 cells, protein levels of phosphorylated AMP-activated protein kinase (AMPK) increased with Dio treatment in a dose-dependent manner. …

    Journal of Nutritional Science and Vitaminology 65(5), 421-429, 2019

    J-STAGE  Ichushi Web 

  • <i>Lactobacillus curvatus</i> CP2998 Prevents Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubes

    KATSUKI Ryo , SAKATA Shinji , NAKAO Reiko , OISHI Katsutaka , NAKAMURA Yasunori

    … CP2998 (CP2998) inhibits glucocorticoid-induced myotube atrophy which is associated with the ubiquitin-proteasome system, mouse skeletal muscle C2C12 myotubes were treated with dexamethasone (DEX) in the presence or absence of CP2998. … DEX exposure significantly decreased myotube diameters and increased mRNA expression levels of <i>MuRF1</i> …

    Journal of Nutritional Science and Vitaminology 65(5), 455-458, 2019

    J-STAGE  Ichushi Web 

  • Activation of adiponectin receptors has negative impact on muscle mass in C2C12 myotubes and fast-type mouse skeletal muscle

    Ito Rika , Higa Masaki , Goto Ayumi , Aoshima Megumi , Ikuta Akihiro , Ohashi Kazuya , Yokoyama Shingo , Ohno Yoshitaka , Egawa Tatsuro , Miyata Hirofumi , Goto Katsumasa

    … This study investigated the effects of AdipoRon, which is an agonist for adiponectin receptor 1 (AdipoR1) and AdipoR2, on the protein content, myotube diameter, and number of nuclei per myotube of C2C12 cells and skeletal muscle mass in C57BL/6J mice. …

    PLOS ONE 13(10), 2018-10-11

    IR 

  • Automatic Quantitative Segmentation of Myotubes Reveals Single-cell Dynamics of S6 Kinase Activation

    Inoue Haruki , Kunida Katsuyuki , Matsuda Naoki , Hoshino Daisuke , Wada Takumi , Imamura Hiromi , Noji Hiroyuki , Kuroda Shinya

    … However, a segmentation method for elongated polynuclear cells, such as differentiated C2C12 myotubes, has yet to be developed. … We used time-lapse fluorescence images of differentiated C2C12 cells stably expressing Eevee-S6K, a fluorescence resonance energy transfer (FRET) biosensor of S6 kinase (S6K). … Summation of binary images enhanced the contrast between myotubes and reserve cells, permitting detection of a myotube and a myotube center. …

    2018-08-31

    IR 

  • Effect of Cyclic Tensile Stimuli on Tissue Maturation of Myoblast-Gel Fibers  [in Japanese]

    BANSAI Shinako , MORIKURA Takashi , ZENMYO Daiki , MIYATA Shogo

    … The purpose of this study is to form mature myotubular tissue using C2C12 cells, a mouse skeletal muscle myoblast cell line, <i>in vitro</i>. … Cyclic stretch was used for tissue maturation to enlarge myotube cells. …

    The Proceedings of the Symposium on Micro-Nano Science and Technology 2018.9(0), 31am3PN117, 2018

    J-STAGE 

  • Functional reorganization of excitable cell culture system for screening novel fluorescent probes  [in Japanese]

    NOMURA YASUTOMO , YAMATO HIRO , IIDA TATSUTO , JIN TAKASHI

    … 最近、骨格筋由来C2C12が筋芽細胞から筋管細胞への形態的な分化に続いて、数時間の電気刺激がサルコメア形成を促し電気刺激に対する応答性を獲得できることが報告された。 …

    Transactions of Japanese Society for Medical and Biological Engineering Annual56(Abstract), S38-S38, 2018

    J-STAGE 

  • Automatic Quantitative Segmentation of Myotubes Reveals Single-cell Dynamics of S6 Kinase Activation

    Inoue Haruki , Kunida Katsuyuki , Matsuda Naoki , Hoshino Daisuke , Wada Takumi , Imamura Hiromi , Noji Hiroyuki , Kuroda Shinya

    … However, a segmentation method for elongated polynuclear cells, such as differentiated C2C12 myotubes, has yet to be developed. … We used time-lapse fluorescence images of differentiated C2C12 cells stably expressing Eevee-S6K, a fluorescence resonance energy transfer (FRET) biosensor of S6 kinase (S6K). … Summation of binary images enhanced the contrast between myotubes and reserve cells, permitting detection of a myotube and a myotube center. …

    Cell Structure and Function 43(2), 153-169, 2018

    J-STAGE 

  • The Extract of Soybean Protein Increases Slow-Myosin Heavy Chain Expression in C2C12 Myotubes

    SANEYASU Takaoki , SHINDO Haruka , HONDA Kazuhisa , KAMISOYAMA Hiroshi

    … In this study using C2C12 myotubes, an extract of soybean protein significantly upregulated mRNA level of myosin heavy chain 7 (<i>Myh7</i>), the predominant isoform expressed in oxidative slow-twitch type I and downregulated mRNA levels of <i>Myh4</i>, the predominant isoform expressed in glycolytic fast-twitch IIB. …

    Journal of Nutritional Science and Vitaminology 64(4), 296-300, 2018

    J-STAGE  Ichushi Web 

  • Functional reorganization of excitable cell culture system for screening novel fluorescent probes in NIR-II  [in Japanese]

    Tomo Ryoto , Jin Takashi , Nomura Yasutomo

    C2C12 skeletal myoblasts were morphologically differentiated to myotubes under the optimal conditions, and the electrical stimulation-induced contraction of the myotubes was monitored optically. … This myotube system would permit screening membrane potential dyes in NIR-II. …

    BULLETIN OF MAEBASHI INSTITUTE OF TECHNOLOGY 21(0), 37-40, 2018

    J-STAGE 

  • The Major Lysosomal Membrane Proteins LAMP-1 and LAMP-2 Participate in Differentiation of C2C12 Myoblasts

    Sakane Hiroshi , Akasaki Kenji

    … In this study, to understand the role of LAMP proteins in the course of differentiation of myoblasts into myotubes, we used C2C12 myoblasts and found that the protein and mRNA levels of LAMP-1 and LAMP-2 were increased in the course of differentiation of C2C12 myoblasts into myotubes. …

    Biological and Pharmaceutical Bulletin 41(8), 1186-1193, 2018

    J-STAGE  Ichushi Web 

  • <b>Effect of Low-intensity Pulsed Ultrasound on Differentiation of C2C12 Cells</b>  [in Japanese]

    SHIMODE Akira , KAJIMOTO Tadamori , YAMAZOE Mitsuyoshi , HOTTA Masato

    … 調べるために,<i>in vitro</i>で筋細胞に分化することができるマウスC2C12細胞にLIPUS照射が与える影響について検討した.</p><p>その結果,3MHzの周波数のLIPUS照射では90mW/cm<sup>2</sup>まで出力を上げても細胞障害性はなかった.さらに,3MHzの周波数,出力70mW/cm<sup>2</sup>,15分間のLIPUS照射はC2C12細胞(筋芽細胞)が筋細胞に分化する過程を促進し,1日1回の照射は7日間連 …

    Journal of Japanese Society of Oral Implantology 31(2), 153-161, 2018

    J-STAGE  Ichushi Web 

  • The effect of advanced glycation end products on cellular signaling molecules in skeletal muscle

    Egawa Tatsuro , Ohno Yoshitaka , Yokoyama Shingo , Goto Ayumi , Ito Rika , Hayashi Tatsuya , Goto Katsumasa

    … Treatment of C2C12 skeletal muscle cells with glucose-induced AGEs (0.1 mg/mL) for 5 days suppressed myotube formation, and this was accompanied by Nε-carboxymethyl-lysine accumulation. …

    The Journal of Physical Fitness and Sports Medicine 7(4), 229-238, 2018

    J-STAGE 

  • S100B impairs glycolysis via enhanced poly(ADP-ribosyl)ation of glyceraldehyde-3-phosphate dehydrogenase in rodent muscle cells

    Hosokawa Kaori , Hamada Yoji , Fujiya Atsushi , Murase Masatoshi , Maekawa Ryuya , Niwa Yasuhiro , Izumoto Takako , Seino Yusuke , Tsunekawa Shin , Arima Hiroshi

    … To clarify the role of S100B in insulin action and glucose metabolism in peripheral tissues, we investigated the effect of S100B on glycolysis in myoblast and myotube cells. … Similar results were obtained in C2C12 myotube cells. …

    American Journal of Physiology - Endocrinology and Metabolism 312(6), E471-E481, 2017-06-01

    IR 

  • Measurement of Contractile Force of Myotube on Micro-pattern by Electrical Stimulation  [in Japanese]

    WATANABE Daiki , HASHIMOTO Shigehiro

    The Proceedings of Conference of Kanto Branch 2017.23(0), 1410, 2017

    J-STAGE 

  • Effects of Co-Transfection with Myostatin-Targeting siRNA and ActRIIB-Fc Fusion Protein on Skeletal Muscle Growth

    BAYARSAIKHAN Od , KAWAI Nobuhiko , MORI Hiroyo , KINOUCHI Nao , NIKAWA Takeshi , TANAKA Eiji

    C2C12 cells were treated by Mstn-siRNA with or without ActRIIB-Fc at 0 and 48 h after differentiation. … Myotube size was measured, and gene expression of <i>Mstn, MuRF-1, MyoD</i> … Transfection of Mstn-siRNA and Mstn-siRNA/ActRIIB-Fc resulted in signi.cant increases in the myotube diameter of the C2C12 cells compared with untreated control. …

    Journal of Oral Health and Biosciences 30(1), 1-7, 2017

    IR  J-STAGE  Ichushi Web 

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