Search Results 1-20 of 158

  • Expression of a Constitutively Active Form of Hck in Chondrocytes Activates Wnt and Hedgehog Signaling Pathways, and Induces Chondrocyte Proliferation in Mice

    Matsuura Viviane K. S. Kawata , Yoshida Carolina Andrea , Komori Hisato , Sakane Chiharu , Yamana Kei , Jiang Qing , Komori Toshihisa

    … Runx2 is required for chondrocyte proliferation and maturation. … Chondrocyte maturation was delayed until E16.5 but accelerated thereafter. … Furthermore, Hck knock-down reduced the proliferation of primary chondrocytes. …

    International Journal of Molecular Sciences 21(8), 2682, 2020-04-12

    IR 

  • Antxr1, Which is a Target of Runx2, Regulates Chondrocyte Proliferation and Apoptosis

    Jiang Qing , Qin Xin , Yoshida Carolina Andrea , Komori Hisato , Yamana Kei , Ohba Shinsuke , Hojo Hironori , Croix Brad St. , Kawata-Matsuura Viviane K. S. , Komori Toshihisa

    … Runx2 is essential for osteoblast di erentiation and chondrocyte maturation and regulates chondrocyte proliferation through Ihh induction. … However,the limbs of Antxr1–/– mice were shorter than those of wild-type mice from embryonic day 16.5 due to the reduced chondrocyte proliferation. …

    International Journal of Molecular Sciences 21(7), 2425, 2020-03-31

    IR 

  • Retrotransposons Manipulating Mammalian Skeletal Development in Chondrocytes

    Kubota Satoshi , Ishikawa Takanori , Kawata Kazumi , Hattori Takako , Nishida Takashi

    … Along with their proliferation in the genome, retrotransposons inevitably modify host genes around the integration sites, and occasionally create novel genes. … In this review, current knowledge on the evolutional, physiological, and pathological roles of retrotransposons in mammalian chondrocyte differentiation and cartilage development is summarized. …

    International Journal of Molecular Sciences 21(5), 1564, 2020-02-25

    IR 

  • Effects of pentosan polysulfate on cell proliferation, cell cycle progression and cyclin-dependent kinases expression in canine articular chondrocytes

    AKARAPHUTIPORN Ekkapol , BWALYA Eugene C. , KIM Sangho , SUNAGA Takafumi , ECHIGO Ryosuke , OKUMURA Masahiro

    … This study investigated the effects of PPS on cell proliferation, particularly in cell cycle modulation and phenotype promotion of canine articular chondrocytes (AC). … The effect of PPS on cell viability, cell proliferation and cell cycle distribution were analyzed by MTT assay, DNA quantification and flow cytometry. … Chondrocyte phenotype was analyzed by quantitative real-time PCR (qPCR) and glycosaminoglycan (GAG) quantification. …

    Journal of Veterinary Medical Science 82(8), 1209-1218, 2020

    J-STAGE 

  • Alterations in characteristics of canine articular chondrocytes in non-passaged long-term monolayer culture: Matter of differentiation, dedifferentiation and redifferentiation

    AKARAPHUTIPORN Ekkapol , SUNAGA Takafumi , BWALYA Eugene C. , ECHIGO Ryosuke , OKUMURA Masahiro

    … The characteristic changes of chondrocytes during the culture period were evaluated based on the cell morphology, cell proliferation, glycosaminoglycans (GAGs) content, DNA quantification, mRNA expression and ultrastructure of chondrocytes. …

    Journal of Veterinary Medical Science 82(6), 793-803, 2020

    J-STAGE 

  • MiR-140-3p Ameliorates the Progression of Osteoarthritis <i>via</i> Targeting CXCR4

    Ren Tiantian , Wei Peng , Song Qinghua , Ye Zhaohui , Wang Yangjian , Huang Lixin

    … MiRNA-140-3p expression in tissue and CHON-001 chondrocyte cells was determined with quantitative real time (qRT)-PCR. … osteoarthritis model was established by treatment of the chondrocyte cells CHON-001 with interleukin (IL)-1β. … Cell proliferation and apoptosis were measured with cell counting kit-8 (CCK8) and Annexin V/propidium iodide (PI) apoptosis assay, respectively. … IL-1β inhibited the proliferation of CHON-001 cells <i>via</i> …

    Biological and Pharmaceutical Bulletin 43(5), 810-816, 2020

    J-STAGE 

  • Clinical Practice Guidelines for Achondroplasia*

    Kubota Takuo , Adachi Masanori , Kitaoka Taichi , Hasegawa Kosei , Ohata Yasuhisa , Fujiwara Makoto , Michigami Toshimi , Mochizuki Hiroshi , Ozono Keiichi

    … This results in the suppression of chondrocyte differentiation and proliferation, which in turn impairs endochondral ossification and causes short-limb short stature. …

    Clinical Pediatric Endocrinology 29(1), 25-42, 2020

    J-STAGE  Ichushi Web 

  • Morphological and Molecular biology Study of Cranial Base Synchondrosis in Cartilage Calcification Insufficient Rat  [in Japanese]

    竹内 綾 , 永山 元彦 , 田中 政巳 , 渡辺 実 , 天野 均 , 柴田 俊一 , 北井 則行

    CCIラットは、全身性に長管骨の低成長など骨格的な形態異常を示す。これまでの兄妹交配ではCCIラットが約25%の頻度で誕生することから、常染色体劣性遺伝性疾患である可能性が予測されたが、具体的な遺伝様式や病態解明には至っていない。そこで、軟骨内骨化を示す頭蓋底軟骨結合に着目し、SDラットとCCIラットを形態学的ならびに分子生物学的に比較検討した。マイクロCT画像、HE染色およびSOFG染色の結果か …

    岐阜歯科学会雑誌 = The Journal of Gifu Dental Society 46(1), 27-39, 2019-06

    IR 

  • Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocytes

    Charlotte Lepleux , Aurélie Marie-Brasset , Mihaela Temelie , Marion Boulanger , Émilie Brotin , Mary B. Goldring , Christophe Hirtz , Guillaume Varès , Tetsuo Nakajima , Yannick Saintigny , Diana Savu , François Chevalier

    … Following a medium transfer protocol, cell survival, proliferation and DNA damages were quantified in bystander chondrocytes. … A significant and major RIBE response was observed in chondrocyte cells (T/C-28a2) receiving conditioned medium from chondrosarcoma cells (SW1353) irradiated with 0.1 Gy of X-rays and 0.05 Gy of C-ions, resulting in cell survivals of 36% and 62%, respectively. …

    Journal of Cell Communication and Signaling 13(3), 343-356, 2019-03-22

    IR 

  • Chicken knee cartilage extract promotes proliferation of the mouse pre-chondrogenic cell line, ATDC5  [in Japanese]

    及川 宝希 , 中谷 祥恵 , 関根 凌兵 , 古旗 賢二

    … vitro effect of adding chicken knee cartilage extract (CKCE) on the proliferation of chondrocytes.Mouse pre-chondrogenic cell line, ATDC5 was cultured with CKCE or CKCE treated with either protease or chondroitinase, and the cell proliferation was evaluated by the WST-1 assay.The CS purified from shark cartilage and HA purified from cockscomh chicken were used as the control groups.The chondrocyte proliferation was 1.5 times higher in the group with CKCE than in the group …

    Functional Food Research (14), 70-76, 2019-03

    IR 

  • Milk basic protein promotes proliferation and inhibits differentiation of mouse chondrogenic ATDC5 cells

    中谷 祥恵 , 田口 陽介 , 上田 博也 , 石田 祐子 , 森田 如一 , 加藤 健 , 和田 政裕 , 古旗 賢二

    … We therefore investigated the effect of MBP on a chondrocyte cell line, ATDC5. … These results suggested that MBP promoted the proliferation of chondrocytes by suppressing their differentiation toward calcification. …

    Journal of Dairy Science 102(4), 2873-2878, 2019-01

    IR 

  • Effects of Leptin on Differentiation and Proliferation of Chondrocytes

    Zhang Shai , Cong Linlin , Hu Lan , Xiao Jianhua , Zhao Yang , Wang Yifan , Muhammad Ishfaq , Ma Ning , Bie Lianyu , Zhao Lidong , Zhu Zihan , Meng Xinghua

    … Collagen X is a specific marker of hypertrophic and calcified chondrocyte. … We conclude that leptin slightly inhibits the proliferation of ATDC5 cells and affects cartilage-specifically marked proteins and genes. …

    Journal of Hard Tissue Biology 28(1), 51-56, 2019

    J-STAGE  Ichushi Web 

  • Live imaging analysis of the growth plate in a murine long bone explanted culture system

    Hirota Keisho , Yasoda Akihiro , Kanai Yugo , Ueda Yohei , Yamauchi Ichiro , Yamashita Takafumi , Sakane Yoriko , Fujii Toshihito , Inagaki Nobuya

    … As for the factors contributing to the elongation of the growth plate, the displacement length of each chondrocyte was larger in the prehypertrophic or hypertrophic zone than in the proliferative zone. … C-type natriuretic peptide, a known positive stimulator of endochondral bone growth mainly targeting prehypertrophic-hypertrophic zone, augmented all of the factors affecting growth plate elongation, whereas it had little effect on the proliferation of chondrocytes. …

    Scientific Reports (8), 2018-12-01

    IR 

  • Paradoxical roles of ATF6α and ATF6β in modulating disease severity caused by mutations in collagen X

    Forouhan M. , Mori K. , Boot-Handford R.P.

    … In addition, novel functions for each paralogue are described including an ATF6β-specific role in controlling growth plate chondrocyte proliferation. …

    Matrix Biology (70), 50-71, 2018-09

    IR 

  • Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death

    Fujii Toshihito , Hirota Keisho , Yasoda Akihiro , Takizawa Akiko , Morozumi Naomi , Nakamura Ryuichi , Yotsumoto Takafumi , Kondo Eri , Yamashita Yui , Sakane Yoriko , Kanai Yugo , Ueda Yohei , Yamauchi Ichiro , Yamanaka Shigeki , Nakao Kazumasa , Kuwahara Koichiro , Jindo Toshimasa , Furuya Mayumi , Mashimo Tomoji , Inagaki Nobuya , Serikawa Tadao , Nakao Kazuwa

    … Histological analysis revealed that the width of the growth plate, especially that of the hypertrophic chondrocyte layer, was markedly lower and the proliferation of growth plate chondrocytes tended to be reduced in CNP KO rats. …

    PLOS ONE 13(3), e0194812, 2018-03-22

    IR 

  • PERK-mediated translational control is required for collagen secretion in chondrocytes

    Hisanaga Satoshi , Miyake Masato , Taniuchi Shusuke , Oyadomari Miho , Morimoto Masatoshi , Sato Ryosuke , Hirose Jun , Mizuta Hiroshi , Oyadomari Seiichi

    … Perk−/− mice displayed reduced collagen in articular cartilage but no differences in chondrocyte proliferation or apoptosis compared to the findings in wild-type mice. …

    Scientific Reports (8), 773, 2018-01-15

    IR 

  • Chicken knee cartilage extract promotes proliferation of the mouse pre-chondrogenic cell line, ATDC5  [in Japanese]

    Oikawa Tomoki , Nakatani Sachie , Sekine Ryohei , Kobata Kenji

     関節軟骨はコンドロイチン硫酸(CS)やヒアルロン酸(HA)などの細胞外基質(ECM)とECMを産生する軟骨細胞で構成されている.近年,精製した CSおよびHAが関節機能改善を目的に健康食品などとして利用されている.一方,生体内で各 ECM成分は相互作用していることが報告されているが,これらECM混合物の軟骨細胞に対する影響についてはあまり報告されていない.そこで本研究では,軟骨ECM …

    Functional Food Research 14(0), 70-76, 2018

    J-STAGE  Ichushi Web 

  • Effects of Mechanical Vibration on Tissue Morphogenesis for Regenerative Medicine Using Cultured Chondrocytes  [in Japanese]

    NOZAWA Tomoaki , SHIRAISHI Toshihiko

    … To observe the effect on production of cartilage matrices and cell proliferation, immunohistochemical examination with staining proteoglycan was conducted and the numbers of cells were measured in order to control groups and vibration groups be compared. … As a result, thickness of chondrocyte cell sheets was increased by mechanical vibration at 12.5 Hz and 0.5 G, but significant difference was not shown. …

    The Proceedings of the Dynamics & Design Conference 2018(0), 540, 2018

    J-STAGE 

  • Physiological and Pathological Functions of Cl<sup>−</sup> Channels in Chondrocytes

    Yamamura Hisao , Suzuki Yoshiaki , Imaizumi Yuji

    … signaling, pH sensing, mechanotransduction, and cell proliferation in articular chondrocytes. … Although these chondrocyte Cl<sup>−</sup> … Elucidating the physiological and pathological functions of chondrocyte Cl<sup>−</sup> … channels will provide us a more comprehensive understanding of chondrocyte functions and may suggest novel molecular targets of drug development for OA.</p> …

    Biological and Pharmaceutical Bulletin 41(8), 1145-1151, 2018

    J-STAGE  Ichushi Web 

  • Extension of knee immobilization delays recovery of histological damages in the anterior cruciate ligament insertion and articular cartilage in rabbits

    Mutsuzaki Hirotaka , Nakajima Hiromi , Sakane Masataka

    … The chondrocyte apoptosis rate and chondrocyte proliferation rate in the ACL insertion and articular cartilage were analyzed after remobilization. …

    Journal of Physical Therapy Science 30(1), 140-144, 2018

    J-STAGE  Ichushi Web 

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