Search Results 1-20 of 42

  • 1 / 3
  • Construction of an All-in-one Double-conditional shRNA Expression Vector

    KUDO Yoshiko , KIUCHI Yuji , OYAMADA Hideto , MATSUOKA Tomoyuki , INAGAKI Ayami , TSUCHIYA Hiromichi , AO Yoko , KIMURA Atsushi Michael , TSUJI Mayumi , OGUCHI Katsuji

    … To overcome these limitations, we previously constructed a double-conditional short-hairpin RNA (shRNA) expression vector that can regulate shRNA expression in a spatio-temporal manner with a tetracycline-inducible floxed stuffer sequence selectively excised by application of Cre recombinase. …

    The Showa University Journal of Medical Sciences 31(1), 41-50, 2019

    IR  J-STAGE 

  • THE ROLE OF PLATELET-DERIVED GROWTH FACTOR RECEPTOR ß IN THE ACTIVATION OF MOUSE HEPATIC STELLATE CELLS

    Morita Seiichi , Oya Takeshi , Ishii Yoko , Sabit Hemragul , Tokunaga Ayano , Ishizawa Shin , Yamamoto Seiji , Hamashima Takeru , Kurashige Yoichi , Matsushima Takako , Tsukada Kazuhiro , Sasahara Masakiyo

    … Methods: We used the wildtype and mutant mice whose PDGFR-ß gene was flanked by two loxP sequences, and isolated/cultured HSCs from these two mice, respectively. … These cells were infected with Cre recombinase-expressing adenovirus. … Results: After Cre-adenovirus infection, PDGFR-ß expression was depleted in HSCs from mutant mice (PDGFR-ßΔ/Δ), but was preserved in those from wild-type. …

    Toyama medical journal 27(1), 14-22, 2017-03

    IR 

  • Generation of a transgenic mouse line for conditional expression of human IL-6

    Mori Taiki , Murasawa Yusuke , Ikai Rina , Hayakawa Tomoko , Nakamura Hiroyuki , Ogiso Noboru , Niida Shumpei , Watanabe Ken

    … Here, we report the establishment of a conditional human IL-6 transgenic mouse, LGL-IL6, which conditionally expresses human IL-6 by taking advantage of the well-characterized Cre recombinase drivers. …

    Experimental animals 65(4), 455-463, 2016-10

    IR 

  • Generation of a transgenic mouse line for conditional expression of human IL-6

    Mori Taiki , Murasawa Yusuke , Ikai Rina , Hayakawa Tomoko , Nakamura Hiroyuki , Ogiso Noboru , Niida Shumpei , Watanabe Ken

    … Here, we report the establishment of a conditional human IL-6 transgenic mouse, LGL-IL6, which conditionally expresses human IL-6 by taking advantage of the well-characterized Cre recombinase drivers.</p> …

    Experimental Animals 65(4), 455-463, 2016

    J-STAGE 

  • Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells

    Matsuyama Rima , Tsutsui Tomomi , Lee Kyoung Ho , Onitsuka Masayoshi , Omasa Takeshi

    … Here, we constructed high-producing and stable cells by the conditional expression of mutant cell division cycle 25 homolog B (CDC25B) using the Cre-loxP system. … After inducing gene amplification in the presence of 250 nM methotrexate, mutant CDC25B sequence was removed by Cre recombinase protein expression. …

    Journal of bioscience and bioengineering 120(6), 701-708, 2015-12

    DOI  Ichushi Web 

  • Somatic chromosomal translocation between Ewsr1 and Fli1 loci leads to dilated cardiomyopathy in a mouse model

    Tanaka Miwa , Yamaguchi Shuichi , Yamazaki Yukari , Kinoshita Hideyuki , Kuwahara Koichiro , Nakao Kazuwa , Jay Patrick Y. , Noda Tetsuo , Nakamura Takuro

    … A mouse model that recapitulates the human Ewing's sarcoma-specific chromosomal translocation was generated utilizing the Cre/loxP-mediated recombination technique. … A cross between Ewsr1-loxP and Fli1-loxP mice and expression of ubiquitous Cre recombinase induced a specific translocation between Ewsr1 and Fli1 loci in systemic organs of both adult mice and embryos. …

    SCIENTIFIC REPORTS (5), 7826, 2015-01-16

    IR 

  • Transgene manipulation in rainbow trout using Cre recombinase

    Kume Sachi , Katayama Naoto , Ichida Kensuke [他]

    Fisheries science 80(4), 767-773, 2014-07

  • A Novel Transgenic Mouse Model Carrying Human Tribbles Related Protein 3 (TRB3) Gene and Its Site Specific Phenotype

    Sakai Yuto , Fukamachi Katsumi , Futakuchi Mitsuru , Miyoshi Ichiro , Tsuda Hiroyuki , Suzui Masumi , Hayashi Hidetoshi

    … morphological changes in a specific organ site we have developed a novel variation of the transgenic mouse model that conditionally expresses human TRB3 (hTRB3) gene using Cre-recombinase (Cre)/loxP recombination system. … The gene expression was controlled by infection of adenovirus-expressing Cre <i>via</i> …

    Biological and Pharmaceutical Bulletin 37(6), 1068-1074, 2014

    J-STAGE  Ichushi Web 

  • Generation and Analysis of Serine Protease Inhibitor Kazal Type 3-Cre Driver Mice

    Sakata Kazuya , Yamamura Ken-ichi , Ohmuraya Masaki , Araki Kimi , Suzuki Chigure , Ida Satoshi , Hashimoto Daisuke , Wang Jung , Uchiyama Yasuo , Baba Hideo

    … or Cre recombinase genes were inserted into the <i>Spink3</i> … locus by Cre-<i>loxP</i> … <i>Spink3<sup>cre</sup></i> … In <i>Spink3<sup>cre</sup></i>;R26R mice, β-galactosidase activity was found in acinar cells of the pancreas, kidney, lung, and a small proportion of cells in the gastrointestinal tract and liver. …

    Experimental Animals 63(1), 45-53, 2014

    J-STAGE  Ichushi Web 

  • Development of primate models for Parkinson's disease due to overexpression of α-synuclein  [in Japanese]

    Takada Masahiko

    … More recently, we have developed a novel parkinsonian model that is subject to α-synuclein overexpression selective to the nigrostriatal dopamine pathway by applying gene manipulation with Cre- loxP site-specific recombination. … First, a lentiviral vector pseudotyped with modified glycoprotein of rabies virus that expresses Cre recombinase was injected into the striatum. …

    Japanese Journal of Biological Psychiatry 24(1), 57-61, 2013

    J-STAGE  Ichushi Web 

  • A rat model of serum diagnosis of lung cancer  [in Japanese]

    FUKAMACHI Katsumi , OHSHIMA Yutaka , FUTAKUCHI Mitsuru , TSUDA Hiroyuki , SUZUI Masumi

    … これまでにCre/loxPシステムを用いた活性型Hras<sup>V12</sup>トランスジェニックラット(Hras250)にCre recombinase発現アデノウイルスベクターを膵臓に投与することにより膵がんを発生させる方法を確立した。 …

    Annual Meeting of the Japanese Society of Toxicology 40.1(0), 150521, 2013

    J-STAGE 

  • Construction of a Novel Single Double-Conditional shRNA Expression Vector

    MATSUOKA Tomoyuki , OYAMADA Hideto , NAKANO Masahideo , SOMEI Masayuki , TOMOYORI Eiji , KONDO Yasuyuki , OGUCHI Katsuji

    … We have generated double-conditional short hairpin RNA from a single vector that can regulate its own transcription by a combination of tetracycline-inducible and Cre-loxP systems. … This vector will be useful for the control of short hairpin RNA expression in a spatially-regulated manner by Cre recombinase as well as a temporally-regulated manner by tetracycline induction. …

    The Showa University Journal of Medical Sciences 25(1), 19-27, 2013

    IR  J-STAGE  Ichushi Web 

  • Novel ROSA26 Cre-reporter Knock-in C57BL/6N Mice Exhibiting Green Emission before and Red Emission after Cre-mediated Recombination

    Hasegawa Yoshikazu , Mekada Kazuyuki , Yoshiki Atsushi , Takahashi Satoru , Sugiyama Fumihiro , Yagami Ken-ichi , Daitoku Yoko , Sekiguchi Keito , Tanimoto Yoko , Mizuno-Iijima Saori , Mizuno Seiya , Kajiwara Noriko , Ema Masatsugu , Miwa Yoshihiro

    … The Cre/loxP system is a strategy for controlling temporal and/or spatial gene expression through genome alteration in mice. … As successful Cre/loxP genome alteration depends on Cre-driver mice, Cre-reporter mice are essential for validation of Cre gene expression <i>in vivo</i>. …

    Experimental Animals 62(4), 295-304, 2013

    J-STAGE  Ichushi Web 

  • Cre/loxP, Flp/FRT Systems and Pluripotent Stem Cell Lines

    Candice G. T. Tahimic , Sakurai Kenji , Aiba Kazuhiro , Nakatsuji Norio

    Cre and FLP recombinases are the most widely used site-specific recombinases in genome engineering. … The substrate sequences for Cre and Flp recombinases are called loxP and FRT sites respectively. … Cre recombinase was discovered in the E. …

    Topics in Current Genetics (23), 189-209, 2013

    IR 

  • 1P-035 Development of a self-excising marker recycling system with a modified intron-containing Cre recombinase in Aspergillus oryzae  [in Japanese]

    Zhang silai , Ban Akihiko , Ebara Naoki , Mizutani Osamu , Tanaka Mizuki , Shintani Takahiro , Gomi Katsuya

    日本生物工学会大会講演要旨集 65, 26, 2013

    NDL Digital Collections 

  • Generation of Human β-thalassemia Induced Pluripotent Stem Cells from Amniotic Fluid Cells Using a Single Excisable Lentiviral Stem Cell Cassette

    FAN Yong , LUO Yumei , CHEN Xinjie , LI Qing , SUN Xiaofang

    … vector flanked by loxP sites, which can be excised with Cre recombinase. …

    Journal of Reproduction and Development 58(4), 404-409, 2012-08-01

    J-STAGE  Ichushi Web  References (17) Cited by (1)

  • Generation of transgenic mice expressing Cre recombinase in arcuate kisspeptin neurons: a tool for region-specific Kiss1 knockout mice

    IKEGAMI Kana , GOTO Teppei , ABE HItomi , HIRABAYASHI Masumi , TOMITA Koichi , SAMBO Makoto , MAEDA Keichiro , TSUKAMURA Hiroko , UENOYAMA Yoshihisa

    … そこで本研究では,Cre-loxPシステムによるARCキスペプチン遺伝子 (<i>Kiss1</i>) のコンディショナルノックアウトマウスの作成の一環として,ARCのキスペプチンニューロン特異的にCreリコンビナーゼを発現するトランスジェニック (Tg) マウスを作製した。 …

    The Journal of Reproduction and Development Supplement 105(0), 1024, 2012

    J-STAGE 

  • Expression and function of homeo domain transcript factor pKnox1 during the oogenesis  [in Japanese]

    KAWAI Yasuhiro , KOIKE Hiroko , AOKI Yusuke , GOITSUKA Ryo

    … 機能を明らかにする為に、<i>Pknox1</i>遺伝子のエクソン2を含む1.5 kbの領域をloxP配列で挟んだ<i>Pknox1<SUP>flox/flox</SUP></i>マウスを作製し、生殖細胞特異的にCre recombinaseを発現する<i>TNAP-Cre</i>マウスとの交配で得られた<i>TNAP-Cre;Pknox1<SUP>flox/flox</SUP></i>マウスを用いて、組織学的解析を行った。 …

    The Journal of Reproduction and Development Supplement 104(0), 308-308, 2011

    J-STAGE 

  • Efficient gene transduction in HER2-expressing cancer cells by two recombinant adenovirus vectors with HER2 promoter and Cre/loxP system

    Nodagashira Tatsuya , Odagiri Hiroki , Ikenaga Shojiro-Kazunori , Maruyama Masaki , Sato Toshiyuki , Hakamada Kenichi

    … For more effi cient gene therapy of HER2-expressing tumor, a double adenovirus infectionsystem was established, in which a 'regulator' vector carried Cre gene under the control of HER2 promoter and 'target'vectors carried target genes activated by Cre. … We constructed a Cre recombinase expression vector, AxHER2Cre, forthe 'regulator' vector. …

    Hirosaki medical journal 61(1), 26-34, 2010

    IR  Ichushi Web 

  • Efficient Gene Disruption in the High-Ploidy Yeast Candida utilis Using the Cre-loxP System

    IKUSHIMA Shigehito , FUJII Toshio , KOBAYASHI Osamu

    … based on the Cre-<I>loxP</I> … For this purpose, two plasmids were constructed: one harbored a heterologous <I>loxP</I>-flanked selection marker cassette carrying the gene responsible for hygromycin B-resistance, and the other had an autonomous replication sequence (ARS) and a Cre-recombinase expression module. …

    Bioscience, Biotechnology, and Biochemistry 73(4), 879-884, 2009-04-23

    J-STAGE  References (37) Cited by (5)

  • 1 / 3
Page Top