Search Results 1-20 of 134

  • CRISPR-Knockout Screen Identifies Dmap1 as a Regulator of Chemically Induced Reprogramming and Differentiation of Cardiac Progenitors

    Yu Jason S. L. , Palano Giorgia , Lim Cindy , Moggio Aldo , Drowley Lauren , Plowright Alleyn T. , Bohlooly-Y Mohammad , Rosen Barry S. , Hansson Emil M. , Wang Qing-Dong , Yusa Kosuke

    Direct in vivo reprogramming of cardiac fibroblasts into myocytes is an attractive therapeutic intervention in resolving myogenic deterioration. … Chemical reprogramming has been established as a legitimate method to generate functional cell types, including those of the cardiac lineage. …

    STEM CELLS 37(7), 958-972, 2019-07


  • Proposal and Investigations on Identification Method of Order of Singularity around Singular Point of Bonded Structures Using Displacement Values  [in Japanese]

    KURAHASHI Takahiko , YAMAGIWA Kengo

    … In general, the adjoint variable and the direct differentiation methods are employed for the inverse analysis. … Therefore, the finite element procedure using Akin's singular element is employed for the discretization of the governing equation, and the finite element equation including the order of singularity is used for the formulation of the identification problem based on the adjoint variable method. …

    Journal of the Society of Materials Science, Japan 67(8), 781-788, 2018


  • An injectable non-cross-linked hyaluronic-acid gel containing therapeutic spheroids of human adipose-derived stem cells

    Feng Jingwei , Mineda Kazuhide , Wu Szu-Hsien , Mashiko Takanobu , Doi Kentaro , Kuno Shinichiro , Kinoshita Kahori , Kanayama Koji , Asahi Rintaro , Sunaga Ataru , Yoshimura Kotaro

    … We describe an easy method for the 3D culture of adipose-derived stem/stromal cells (ASCs) to prepare a ready-to-use injectable. … In irradiated skin ulcers in immunodeficient mice, ASC spheroids exhibited faster healing and outstanding angiogenic potential partly by direct differentiation into α-SMA+ pericytes. …

    Scientific Reports (7), 1548, 2017-05-08


  • Simple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectors

    Goto Kazuya , Imamura Keiko , Komatsu Kenichi , Mitani Kohnosuke , Aiba Kazuhiro , Nakatsuji Norio , Inoue Makoto , Kawata Akihiro , Yamashita Hirofumi , Takahashi Ryosuke , Inoue Haruhisa

    センダイウイルスベクターを用いてES細胞/iPS細胞から脊髄運動ニューロンを簡便に作製する技術開発. 京都大学プレスリリース. 2017-02-07.

    Molecular Therapy - Methods & Clinical Development (4), 115-125, 2017-03-17


  • 1D24 Measurement on three-dimensional mechanical field between cell and extracellular matrix in cellular differentiation using digital volume correlation method  [in Japanese]

    MORITA Yasuyuki , KAWASE Naoki , JU Yang , YAMAUCHI Takashi

    … As regarding cellular differentiation, it has been found that elasticity of extracellular matrix determines differentiation lineage of mesenchymal stem cells (MSCs). … The direct quantitative measurement of the mechanical interaction between the MSC and the matrix for differentiation, however, has not been performed. …

    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2016.28(0), _1D24-1_-_1D24-4_, 2016


  • Decreased expression of lysophosphatidylcholine (16:0/OH) in high resolution imaging mass spectrometry independently predicts biochemical recurrence after surgical treatment for prostate cancer.

    Goto Takayuki , Terada Naoki , Inoue Takahiro , Kobayashi Takashi , Nakayama Kenji , Okada Yoshiyuki , Yoshikawa Takeshi , Miyazaki Yu , Uegaki Masayuki , Utsunomiya Noriaki , Makino Yuki , Sumiyoshi Shinji , Yamasaki Toshinari , Kamba Tomomi , Ogawa Osamu

    … Lipid metabolism affects numerous cellular processes, including cell growth, proliferation, differentiation, and motility. … Direct profiling of lipids in tissue using high-resolution matrix-assisted laser desorption/ionization imaging mass spectrometry (HR-MALDI-IMS) may provide molecular details that supplement tissue morphology. …

    The Prostate 75(16), 1821-1830, 2015-12


  • Human iPS cell-derived fibroblast-like cells as feeder layers for iPS cell derivation and expansion

    Du Shou-Hui , Tay Johan Chin-Kang , Chen Can , Tay Felix-Chang , Tan Wee-Kiat , Li Zhen-Dong , Wang Shu

    … We developed a new method for the derivation of human fibroblast-like cells (FLCs) from a previously established hiPSC line in an FLC differentiation medium. … The method was based on direct differentiation of hiPSCs seeded on Matrigel followed by expansion of differentiating cells on gelatin. … The pluripotency and differentiation capability of the cells cultured on FLC feeder layers were well retained. …

    Journal of bioscience and bioengineering 120(2), 210-217, 2015-08

    DOI  Ichushi Web 

  • Strategies for Heart Regeneration:Approaches Ranging from Induced Pluripotent Stem Cells to Direct Cardiac Reprogramming

    Yamakawa Hiroyuki , Ieda Masaki

    … however, these stem cells demonstrate variable cardiac differentiation efficiency and tumorigenicity, which should be solved for clinical applications. … However, in 2010, we reported for the first time a novel method of the direct reprogramming of fibroblasts into cardiomyocytes, demonstrating various reprogramming pathways exist. …

    International Heart Journal 56(1), 1-5, 2015

    J-STAGE  Ichushi Web 

  • Direct reprogramming based on transcriptional regulatory network analysis  [in Japanese]

    Suzuki Takahiro , Suzuki Harukazu

    … Trans-differentiation of cells through direct reprogramming offers significant potential for regenerative medicine and drug screen, but currently it requires exhaustive trials and errors before the desired target cells can be created. …

    Inflammation and Regeneration 34(5), 224-232, 2014

    J-STAGE  Ichushi Web 

  • Differentiation of Human Induced Pluripotent Stem Cells into Functional Enterocyte-like Cells Using a Simple Method

    IWAO Takahiro , TOYOTA Masashi , MIYAGAWA Yoshitaka , OKITA Hajime , KIYOKAWA Nobutaka , AKUTSU Hidenori , UMEZAWA Akihiro , NAGATA Kiyoshi , MATSUNAGA Tamihide

    …   Human induced pluripotent stem (iPS) cells were differentiated into the endoderm using activin A and were then treated with fibroblast growth factor 2 (FGF2) for differentiation into intestinal stem cell-like cells. …

    Drug Metabolism and Pharmacokinetics 29(1), 44-51, 2014

    J-STAGE  Ichushi Web 

  • OS0610 Study on the measurement of 3D mechanical field toward an elucidation of cell-ECM interaction  [in Japanese]

    KAWASE Naoki , MORITA Yasuyuki , JU Yang

    … Mechanical interactions between cells and extracellular matrix (ECM) direct many important cellular processes such as proliferation, migration and differentiation. … Therefore digital volume correlation (DVC), which is a method of measuring 3D displacement and strain fields within an object by using a pair of 3D volume images, is expected to be able to become a novel tool. …

    The Proceedings of the Materials and Mechanics Conference 2014(0), _OS0610-1_-_OS0610-2_, 2014


  • 5-3-1-454 Effects of dynamic stimulations on differentiating mouse iPS cells  [in Japanese]

    KOIZUMI Tomoyuki , UGAJIN Kazuya , KOSAWADA Tadashi , FENG Zhonggang

    … In this study, we examine the effects of dynamic stimulation by three-dimensional actuator in the process of neuronal differentiation of mouse iPS cells. … This probe is improved to direct contact with cell colonies. … Mouse iPS cells are differentiated into neuron cells using the method of SFEBq. … In the experiment, dynamic stimulation of indentation is indicating promotion of differentiation. …

    Dynamics and Design Conference : D & D 2013, "454-1"-"454-9", 2013-08-25

  • Differentiation of Lard, Chicken Fat, Beef Fat and Mutton Fat by GCMS and EA-IRMS Techniques

    Ahmad Nizar Nina Naquiah , Nazrim Marikkar Jalaldeen Mohamed , Hashim Dzulkifly Mat

    … The direct comparisons among the fatty acid data, therefore, may not be suitable for discrimination of different animal fats. … This would lead to a faster and more efficient method to ascertain the source of origin of fats used in food products. …

    Journal of Oleo Science 62(7), 459-464, 2013


  • Hydroxyapatite/Collagen Bone-Like Nanocomposite

    Kikuchi Masanori

    … self-organization mechanism between them using simultaneous titration method under controlled pH and temperature. … Bone marrow cells co-cultured with osteoblasts on HAp/Col differentiated to osteoclasts without differentiation supplements. … In addition to the above, HAp/Col coating on titanium revealed higher osteoconductivity than HAp-coated titanium and bare titanium and improved direct bonding between titanium and newly formed bone. …

    Biological and Pharmaceutical Bulletin 36(11), 1666-1669, 2013

    J-STAGE  Ichushi Web 

  • Electron Microscopic Study of <i>Kudoa septempunctata</i> Infecting <i>Paralichthys olivaceus</i> (Olive Flounder)

    Ohnishi Takahiro , Furusawa Hiroko , Yoshinari Tomoya , Yamazaki Akiko , Horikawa Kazumi , Kamata Yoichi , Sugita-Konishi Yoshiko

    … spores in the causative fish at food poisoning outbreaks, both a direct observation method using microscopy and a quantitative real-time PCR (qRT-PCR) method are officially accepted in Japan. … However, lower correlations have been often noticed between the number of spores counted using the direct observation method and the DNA amount determined using the qRT-PCR method. …

    Japanese Journal of Infectious Diseases 66(4), 348-350, 2013

    J-STAGE  Ichushi Web 

  • Cardiomyocyte Regeneration and Differentiation Switch  [in Japanese]

    Kawaguchi Nanako

    心筋梗塞は、冠動脈の血管が閉塞することによって、心筋細胞まで酸素や栄養素が運搬されなくなり、心筋細胞が壊死に陥ることにより、心臓の機能が異常になり、発症する。2003年頃、心臓内に、幹細胞が存在することが報告され、心臓内の幹細胞を用いた心筋細胞の再生が心筋梗塞の治療に注目されるようになった。著者のグループも、将来心筋再生医療につながることを目標とした心臓内幹細胞の基礎研究を行ってきた。2006年、 …

    Journal of The Society of Japanese Women Scientists 13(1), 14-22, 2013


  • OS1815 Development of DVC Method for the Measurement of Mechanical Field in Cellular Microenvironment  [in Japanese]

    KAWASE Naoki , MORITA Yasuyuki , JU Yang

    … Mechanical interactions between cells and extracellular matrix (ECM) direct many important cellular processes such as proliferation, migration and differentiation. …

    The Proceedings of the Materials and Mechanics Conference 2013(0), _OS1815-1_-_OS1815-3_, 2013


  • An Algebraic Method to Identify Classes of Formulas in Calculus of Predicates(<Special Issue>Bilevel Programming, Optimization Methods, and Applications to Economics)


    … The suggested method is alternative to the traditional methods of direct and inverse logic deduction in particular to the Robinson's resolution principle. … The deterministic algorithm of polynomial complexity based on Boolean algebra of cubic functions for differentiation between two classes of formulas: valid and satisfiable, or unsatisfiable and satisfiable, is suggested. …

    International Journal of Biomedical Soft Computing and Human Sciences: the official journal of the Biomedical Fuzzy Systems Association 18(1), 69-76, 2013


  • Transplantation of Adipose Tissue-Derived Stem Cells Overexpressing Heme Oxygenase-1 Improves Functions and Remodeling of Infarcted Myocardium in Rabbits

    Yang Jun-jie , Yang Xia , Liu Zhi-qiang , Hu Shun-yin , Du Zhi-yan , Feng Lan-lan , Liu Jian-feng , Chen Yun-dai

    … Due to limited improvement concerning direct stem cell therapy, gene-transfer methods are used to enhance cellular cardiomyoplasty efficacy. … Structural consequences of transplantation were determined by detailed histological analysis, which showed differentiation of HO-1-ADSCs to cardiomyocyte-like tissues and lumen-like structure organizations. …

    The Tohoku Journal of Experimental Medicine 226(3), 231-241, 2012



    TAKAMATSU Toru , TOUKAI Kouichi , USHIMARU Shinya , MATSUMOTO Satohiro , IWAKI Takaaki , FUKUNISHI Masanori , SAGIHARA Noriyoshi , MIYATANI Hiroyuki , YOSHIDA Yukio , YAMADA Shigeki

    食道粘膜下腫瘍(SMT)は良性腫瘍が大部分を占め,悪性は稀とされている.しかし,画像所見のみでは良悪性の鑑別が困難な場合もあり,組織型により治療方針も異なるため組織診断を得ることが必要である.今回,われわれは30mmの食道SMTに対して超音波内視鏡下穿刺吸引生検術(EUS-FNAB)を試みたが,組織量が不十分で確定診断に至らず,粘膜切開後直視下生検にて確定診断が得られた症例を経験した.EUS-FN …

    GASTROENTEROLOGICAL ENDOSCOPY 53(9), 3023-3027, 2011-09-20

    J-STAGE  Ichushi Web  References (10)

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