Search Results 1-20 of 91

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  • Malic Enzyme Facilitates d-Lactate Production through Increased Pyruvate Supply during Anoxic Dark Fermentation in Synechocystis sp. PCC 6803

    Hidese Ryota , Matsuda Mami , Osanai Takashi , Hasunuma Tomohisa , Kondo Akihiko

    Dynamic metabolomics revealed that overexpression of ME led to increased turnover rates in malate and pyruvate metabolism; … Finally, both the loss of the acetate kinase gene and overexpression of endogenous d-lactate dehydrogenase, concurrent with ME overexpression, resulted in the highest production of d-lactate (26.6 g/L) with an initial cell concentration of 75 g-DCW/L after 72 h fermentation. …

    ACS Synthetic Biology 9(2), 2020-01-31


  • Development of Worker's Nonstandard Motion Detection System in Cell Production Line  [in Japanese]

    Nishida Kazuki , Onda Hiroomi

    … <p>In this paper, we propose a method to detect workers' nonstandard motion in cell production lines. … Our method consists of three features: (1) sensing motion of a person, (2) measuring the time of motion by using Dynamic Time Warping and (3) analysis of the motion time. … We applied the method to a prototype cell production line in our factory and verified its effectiveness. …

    Transactions of the Institute of Systems, Control and Information Engineers 33(5), 149-155, 2020


  • Single-Stage Astaxanthin Production Enhances the Nonmevalonate Pathway and Photosynthetic Central Metabolism in Synechococcus sp. PCC 7002

    Hasunuma Tomohisa , Takaki Ayako , Matsuda Mami , Kato Yuichi , Vavricka Christopher J. , Kondo Akihiko

    … The green alga Haematococcus pluvialis is currently used for commercial production of astaxanthin pigment. … PCC 7002 (Synechococcus 7002) is able to reach high density under stress-free phototrophic conditions, and is therefore a promising metabolic engineering platform for astaxanthin production. …

    ACS synthetic biology 8(12), 2701-2709, 2019-10-25


  • Project 2 Development on Neutron Imaging Application (30P2)

    Saito Y.

    … Ito (30P2-1) [14]PR2-2 Simultaneous Measurements of Water Distribution and Electrochemical Characteristics in Polymer Electrolyte Fuel Cell /H. … Tsukada et al.(30P2-4) [17]PR2-5 Quantitative Dynamic Measurement of Void Fraction /H. … Matsumoto et al.(30P2-6) [19]PR2-7 Analysis of Production Technology of Optics Components Using Neutron Radiography /Y. …

    KURNS Progress Report (2018), 12-25, 2019-08


  • 2C01 Testing using the Experimental Equipment for Smart Factory on Vocational Training  [in Japanese]

    SATO Takashi , FURUI Hidenori , TAKAHASHI Koji

    Proceedings of Annual Conference of Japanese Society for Engineering Education 2019(0), 178-179, 2019


  • Membrane Dynamics Induced by a Phosphatidylinositol 3,4,5-Trisphosphate Optogenetic Tool

    UEDA Yoshibumi , II Tatsuhito , AONO Yuki , SUGIMOTO Naotoshi , SHINJI Seiichi , YOSHIDA Hiroshi , SATO Moritoshi

    … <p>Membrane dynamic structures such as filopodia, lamellipodia, and ruffles have important cellular functions in phagocytosis and cell motility, and in pathological states, such as cancer metastasis. … production. … generation induced directional cell motility and membrane ruffles in COS7 cells. …

    Analytical Sciences 35(1), 57-63, 2019

    J-STAGE  Ichushi Web 

  • Disruption of mitochondrial dynamics by azidothymidine-triphosphate causes lethal cardiomyopathy

    Sato Takeya , Nomura Ryosuke , Kushimoto Shigeki , Yanagisawa Teruyuki

    … Mitochondria are dynamic, mobile organelles that continuously change their shape and size through repeated cycles of fission (which allows the removal of damaged mitochondria) and fusion (to repair damaged components) to maintain normal cellular function. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO4-9-20, 2018


  • Beta-arrestin2 deficiency protects against hepatic fibrosis in mice and prevents synthesis of extracellular matrix

    Sun Wu-Yi , Gu Yuan-Jing , Li Xin-Ran , Peng Wen-Ting , Chen Jing-Yu , Ma Yang , Wang Qing-Tong , Wei Wei

    … <p>Background: beta-arrestin2 is a multifunctional adaptor protein, which has recently been found to play new roles in regulating intracellular signalling networks associated with cell functions. … The dynamic expressions of beta-arrestin2, collagen I, collagen III, tissue inhibitor of metalloproteinase-1 (TIMP-1), matrix metalloproteinase-13 (MMP-13) in rat liver tissues were measured by immunohistochemical staining and Western blot. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO2-6-4, 2018


  • Elevated <i>O</i>-GlcNAcylation in the tumor microenvironment promotes B16 melanoma cell progression through the suppression of p38 MAPK

    Moriwaki Kazumasa , Asahi Michio

    … <p><i>O</i>-GlcNAcylation is a dynamic post-translational modification of cytonuclear proteins for a wide range of intracellular signaling. … Elevated <i>O</i>-GlcNAcylation is a general feature of cancer cell and contributes to cancer progression, and recent studies indicate the contribution to increasing incidence of various types of cancer in diabetic patients. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO1-8-38, 2018


  • Structural Biological Approach to Biopharmaceuticals  [in Japanese]

    Kato Koichi , Yanaka Saeko , Yagi Hirokazu

    … Most protein biopharmaceuticals are modified with structurally heterogeneous and dynamic oligosaccharides which govern the physicochemical properties, functionality, pharmacokinetics, and potential pathogenicity of these glycoproteins. … Considering this, we have developed a structural biological approach to describe the dynamic three-dimensional structures and interactions of glycoproteins as biopharmaceuticals. …

    YAKUGAKU ZASSHI 138(12), 1495-1502, 2018

    J-STAGE  Ichushi Web 

  • Effect of Mechanical Compression on Progression of In Vitro Three Dimensional Melanoma Model  [in Japanese]


    … ポットに1.6×104 cells/cm2の密度で播種し2日間培養した後,皮膚を模擬して上部から2.4 mg/mlのI型コラーゲン中性溶液を滴下してゲル化させin vitro三次元浸潤モデルを構築した.次に,作製した浸潤モデルの上部にCell Culture Insertを配置し,最上部に任意の重さの円筒状SUS304製錘を置くことで細胞挙動を顕微鏡下で経時的に観測可能な圧縮刺激印加装置を開発した.さらに,定常圧縮刺激の印加時間および負荷荷重によって細 …

    Transactions of Japanese Society for Medical and Biological Engineering Annual56(Abstract), S418-S418, 2018


  • Investigation of the Influence of Slurry Properties and Electrode Microstructure on the Performance of Polymer Electrolyte Fuel Cell  [in Japanese]

    Kishi Michito , Moriyama Shohei , Mori Takamasa

    … <p>In order to understand microstructure formation process of the catalyst layer for a polymer electrolyte fuel cell (PEFC), we tried to evaluate the catalyst slurry properties. … On the other hand, the electrode was fabricated from the prepared slurry and its performance was examined.</p><p>It was shown that the mean diameter of ionomer measured by dynamic light scattering and the adsorbed amount of ionomer on Pt/C particles decreased with an increase in alcohol ratio of solvent. …

    Journal of the Society of Powder Technology, Japan 55(7), 366-374, 2018


  • Shading and electric performance of a prototype greenhouse blind system based on semi-transparent photovoltaic technology

    Zhi LI , YANO Akira , Marco COSSU , YOSHIOKA Hidekazu , KITA Ichiro , IBARAKI Yasuomi

    … To overcome this difficulty, we prototyped a venetian-blind-type shading system comprising semi-transparent bifacial PV modules that concurrently function for greenhouse shading control and electricity production. … This system might be applicable to greenhouses in high-insolation regions as an energetically stand-alone dynamic shading system to produce a better light environment for crops. …

    Journal of Agricultural Meteorology 74(3), 114-122, 2018


  • Vitrification of Biological Cells Using a Cryogenic Fine Solid Particulate Spray


    … We have developed a cryopreservation method that uses a single-component super-adiabatic two-fluid de Laval nozzle for the continuous production of solid nitrogen particles with a micro-nano-order particle size. … The cell viability results showed that use of the solid nitrogen spray improved cell viability at the time of thawing by more than 20% compared with the conventional liquid nitrogen immersion freezing method. …

    Interdisciplinary Information Sciences 23(2), 167-170, 2017

    IR  J-STAGE 

  • A study on operator allocation aiming at the skill improvement for cell production system (4th Report, A proposal of operational method under dynamic conditions)  [in Japanese]

    HARAGUCHI Harumi , KAIHARA Toshiya , FUJII Nobutada , KOKURYO Daisuke

    <p>We proposed a new human index named "skill index" and also proposed operator allocation and scheduling method using this index in our previous study. We confirmed the training effec …

    Transactions of the JSME (in Japanese) 83(848), 16-00357-16-00357, 2017


  • Suppressive Effects of Self-Associated Triblock Copolymers on Quorum Sensing-Mediated Phenazine Antibiotic Production

    Okano Chigusa , Kato Norihiro

    … Effective suppression of a bacterial quorum sensing (QS) could be achieved by adding A-B-A tri-block copolymer, poloxamer 84 or 407 into a culture broth, resulting in decrease of the QS-mediated phenazine production in <i>Pseudomonas chlororaphis</i> … Dynamic light scattering measurements suggested to form self-associated micelles of respective poloxamer, because a single peak in the size distribution was observed under the same concentration (1 wt%) and temperature (30℃) for the QS suppressive test. …

    Transactions of the Materials Research Society of Japan 41(2), 163-168, 2016


  • 2C21 Dynamic Response Evaluation of Porcine Fibula under Three-Point Bending Using Drop Weight Testing Machine  [in Japanese]

    KATO Masashi , PRAMUDITA Jonas A. , TANABE Yuji

    … However, an injury criterion for finger fracture based on experimental data under dynamic loading condition has not yet been proposed. … Present study was conducted in order to investigate the fracture threshold of small-diameter long bone under dynamic bending and pinching loading as a first step in developing an injury criterion. …

    The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME 2016.28(0), _2C21-1_-_2C21-4_, 2016


  • Mdivi-1, mitochondrial fission inhibitor, impairs developmental competence and mitochondrial function of embryos and cells in pigs

    YEON Ji-Yeong , KIM Sun-Uk , CHANG Kyu-Tae , KOO Deog-Bon , MIN Sung-Hun , PARK Hyo-Jin , KIM Jin-Woo , LEE Yong-Hee , PARK Soo-Yong , JEONG Pil-Soo , PARK Humdai , LEE Dong-Seok

    … Mitochondria are highly dynamic organelles that undergo constant fusion/fission as well as activities orchestrated by large dynamin-related GTPases. … These dynamic mitochondrial processes influence mitochondrial morphology, size and function. … Our results showed that the rate of blastocyst production and cell growth in the mdivi-1 (50 μM) treated group was lower than that of the control group (P < …

    Journal of Reproduction and Development 61(2), 81-89, 2015

    J-STAGE  Ichushi Web 

  • Development of a new high-throughput method to determine the composition of ten monosaccharides including 4-<i>O</i>-methyl glucuronic acid from plant cell walls using ultra-performance liquid chromatography

    Sakamoto Shingo , Yoshida Kouki , Sugihara Satoko , Mitsuda Nobutaka

    … Plant cell walls are an important dietary source for livestock, and could be an enormous resource for production of next-generation bioethanol and more valuable materials. … Because polysaccharides are major components of plant cell walls, analysis of their composition is important. …

    Plant Biotechnology 32(1), 55-63, 2015


  • A 3D Sphere Culture System Containing Functional Polymers for Large-Scale Human Pluripotent Stem Cell Production

    Otsuji TomomiG. , Bin Jiang , Yoshimura Azumi , Tomura Misayo , Tateyama Daiki , Minami Itsunari , Yoshikawa Yoshihiro , Aiba Kazuhiro , Heuser JohnE. , Nishino Taito , Hasegawa Kouichi , Nakatsuji Norio

    ヒト多能性幹細胞(ES/iPS細胞)の新たな三次元培養法 -大量培養・大規模生産を可能に-. 京都大学プレスリリース. 2014-04-25.

    Stem Cell Reports 2(5), 734-745, 2014-04


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