Search Results 1-20 of 8730

  • Glioma cells require one-carbon metabolism to survive glutamine starvation

    Tanaka Kazuhiro , Sasayama Takashi , Nagashima Hiroaki , Irino Yasuhiro , Takahashi Masatomo , Izumi Yoshihiro , Uno Takiko , Satoh Naoko , Kitta Akane , Kyotani Katsusuke , Fujita Yuichi , Hashiguchi Mitsuru , Nakai Tomoaki , Kohta Masaaki , Uozumi Yoichi , Shinohara Masakazu , Hosoda Kohkichi , Bamba Takeshi , Kohmura Eiji

    Gene expression analysis identified upregulation of methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) to regulate one-carbon metabolism. … In clinical samples, MTHFD2 expression was highest in the nutrient-poor areas around "pseudopalisading necrosis." Genetic suppression of MTHFD2 and autophagy inhibition caused tumor cell death and growth inhibition of glioma cells upon glutamine deprivation. …

    Acta Neuropathologica Communications 9(1), 16, 2021-12-19

    IR 

  • PAI-1 derived from cancer-associated fibroblasts in esophageal squamous cell carcinoma promotes the invasion of cancer cells and the migration of macrophages

    Sakamoto Hiroki , Koma Yu-ichiro , Higashino Nobuhide , Kodama Takayuki , Tanigawa Kohei , Shimizu Masaki , Fujikawa Masataka , Nishio Mari , Shigeoka Manabu , Kakeji Yoshihiro , Yokozaki Hiroshi

    … To identify molecules associated with the ESCC progression in CAFs, we conducted a cDNA microarray analysis on monocultured MSCs and CAF-like cells to compare their gene expression profiles. … Based on immunohistochemistry assays of ESCC tissues, higher expression levels of PAI-1 and LRP1 were correlated with poor prognosis in ESCC patients. …

    Laboratory Investigation 101(3), 353-368, 2021-03

    IR 

  • Molecular and functional characterization of the novel odorant-binding protein gene AccOBP10 from Apis cerana cerana

    Guo Dezheng , Hao Cuihong , Cui Xuepei , Wang Ying , Liu Zhenguo , Xu Baohua , Guo Xingqi

    The journal of biochemistry 169(2), 215-225, 2021-02

  • SNAIL regulates gastric carcinogenesis through CCN3 and NEFL

    Chen Ru , Masuo Kenji , Yogo Akitada , Yokoyama Shoko , Sugiyama Aiko , Seno Hiroshi , Yoshizawa Akihiko , Takaishi Shigeo

    … Microarray analysis in gastric cancer cell line HGC27 showed that CCN3 and NEFL displayed the greatest differential expression by knocking down of SNAIL; … Downregulation of CCN3 and upregulation of NEFL gene expression impaired the SNAIL-dependent EMT activity: high tumorigenicity, and chemoresistance in gastric cancer cells. …

    Carcinogenesis 49(2), 190-201, 2021-02

    IR 

  • Genetic Elucidation for Response of Flowering Time to Ambient Temperatures in Asian Rice Cultivars

    Hori Kiyosumi , Saisho Daisuke , Nagata Kazufumi , Nonoue Yasunori , Uehara-Yamaguchi Yukiko , Kanatani Asaka , Shu Koka , Hirayama Takashi , Yonemaru Jun-Ichi , Fukuoka Shuichi , Mochida Keiichi

    … this QTL would encode a novel flowering-time gene. … RNA-seq analysis showed differential expression of several flowering-time genes between early and late flowering seasons. …

    International Journal of Molecular Sciences 22(3), 1024, 2021-01-20

    IR 

  • Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells

    Takashima Shinichiro , Usui Soichiro , Inoue Oto , Goten Chiaki , Yamaguchi Kosei , Takeda Yusuke , Cui Shihe , Sakai Yoshio , Hayashi Kenshi , Sakata Kenji , Kawashiri Masa-aki , Takamura Masayuki , 高島 伸一郎 , 薄井 荘一郎 , 井上 己音 , 林 研至 , 川尻 剛照 , 高村 雅之

    … We also identified beating CM-fated progenitors (CFPs) and performed single-cell transcriptome analysis of these CFPs. … Transduction of Ad-SVF cells with Mef2c using a lentiviral vector yielded CFPs and beating CMs with ~ tenfold higher cardiac troponin T expression, which was abolished by silencing of Mef2c. … Thus, we identified the master gene required for transdifferentiation of Ad-SVF into beating CMs. …

    Scientific Reports (11), 1520, 2021-01-15

    IR  DOI 

  • High-resolution analysis of cell-state transitions in yeast suggests widespread transcriptional tuning by alternative starts

    Minghao Chia , Cai Li , Sueli Marques , Vicente Pelechano , Nicholas M. Luscombe , Folkert J. van Werven

    … Yet the contribution of transcript diversity in regulating gene expression remains largely elusive. … We perform an integrative analysis of multiple highly synchronized cell-fate transitions and quantitative genomic techniques in Saccharomyces cerevisiae to identify regulatory functions associated with transcribing alternative isoforms. … Results: Cell-fate transitions feature widespread elevated expression of alternative TSS and, to a lesser degree, TES usage. …

    Genome Biology 22(1), 34, 2021-01-14

    IR 

  • MAGEA10 expression is a predictive marker of early hepatic recurrence after curative gastrectomy for gastric and gastroesophageal junction cancer

    Fujiya Keiichi , Terashima Masanori , Ohshima Keiichi , Aizawa Daisuke , Sugino Takashi , Serizawa Masakuni , Nakamura Kenichi , Nagashima Takeshi , Hatakeyama Keiichi , Urakami Kenichi , Akiyama Yasuto , Tsubosa Yasuhiro , Kitagawa Yuko , Yamaguchi Ken

    Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 24(2), 341-351, 2021

  • MicroRNA-665 Regulates Cell Proliferation and Apoptosis of Vascular Smooth Muscle Cells by Targeting TGFBR1

    Wang Lang , Zhou Jiali , Guo Fan , Yao Tan , Zhang Liang

    … This study aimed at exploring differentially expressed microRNAs (miRNAs) and their regulatory mechanisms in the development of CAD.</p><p>The miRNA expression profile of GSE28858 was obtained from the Gene Expression Omnibus database. … Target genes of DEmiRNAs were predicted, and a miRNA-target gene network was constructed. …

    International Heart Journal 62(2), 371-380, 2021

    J-STAGE 

  • A Novel Titin Truncation Variant Linked to Familial Dilated Cardiomyopathy Found in a Japanese Family and Its Functional Analysis in Genome-Edited Model Cells

    Hirayama-Yamada Kayoko , Inagaki Natsuko , Hayashi Takeharu , Kimura Akinori

    … <i>TTN</i>, which encodes titin protein, is a representative causative gene of DCM, and is presented mainly as a truncation variant. … The cells were differentiated using all-trans-retinoic acid, and the mRNA expression of skeletal actin and cardiac actin were found to be increased and decreased, respectively, consistent with known changes in patients with DCM or heart failure. …

    International Heart Journal 62(2), 359-366, 2021

    J-STAGE 

  • Establishment of spatio-temporal analysis in gene expression level through soybean flowering mechanisms  [in Japanese]

    Taniguchi Takatoshi , Kurose Ryota , Yonekawa Yuki , Noriyuki Syodai , Hamaoka Norimitsu , Ishibashi Yushi

    Abstracts of Meeting of the CSSJ 251(0), 96-96, 2021

    J-STAGE 

  • Gene expression analysis of Na<sup>+</sup> transporters in two rice varieties with different abilities for Na<sup>+</sup> removal in leaf sheaths  [in Japanese]

    Goto Itsuki , Yamauchi Akira , Mitsuya Shiro

    Abstracts of Meeting of the CSSJ 251(0), 135-135, 2021

    J-STAGE 

  • Integrated transcript and metabolite profiling reveals coordination between biomass size and nitrogen metabolism in <i>Arabidopsis</i> F<sub>1</sub> hybrids

    Sugi Naoya , Le Quynh Thi Ngoc , Kobayashi Makoto , Kusano Miyako , Shiba Hiroshi

    … Plant samples grown under the optimized condition were also utilized for integrated omics analysis to capture specific changes in the F<sub>1</sub> … In addition, microarray analysis revealed that there were 44 up-regulated and 12 down-regulated genes with more than 1.5-fold changes in expression levels in the F<sub>1</sub> … Gene ontology (GO) analyses indicated that genes up-regulated in the F<sub>1</sub> …

    Plant Biotechnology 38(1), 67-75, 2021

    J-STAGE 

  • <i>Mesorhizobium</i> sp. J8 can establish symbiosis with <i>Glycyrrhiza uralensis</i>, increasing glycyrrhizin production

    Kusaba Ikuko , Akashi Ryo , Suzuki Akihiro , Nakao Takahiro , Maita Hiroko , Sato Shusei , Chijiiwa Ryota , Yamada Emi , Arima Susumu , Kojoma Mareshige , Ishimaru Kanji

    … Whole-genome analysis revealed a single circular chromosome of 6.7 Mbp. … by phylogenetic analysis and was designated <i>Mesorhizobium</i> … Furthermore, the expression level of the <i>CYP88D6</i> … gene, which is a marker of GL synthesis, was 2.5 times higher than in inoculated plants. …

    Plant Biotechnology 38(1), 57-66, 2021

    J-STAGE 

  • Possible roles for the hominoid-specific DSCR4 gene in human cells

    Saber Morteza M. , Karimiavargani Marziyeh , Uzawa Takanori , Hettiarachchi Nilmini , Hamada Michiaki , Ito Yoshihiro , Saitou Naruya

    … DSCR4 (Down syndrome critical region 4) is a <i>de novo</i>-originated protein-coding gene present only in human chromosome 21 and its homologous chromosomes in apes. … We used a bioinformatic approach to infer the biological importance and cellular roles of this gene. … Our analysis indicates that DSCR4 is likely involved in the regulation of interconnected biological pathways related to cell migration, coagulation and the immune system. …

    Genes & Genetic Systems, 2021

    J-STAGE 

  • Molecular pathogenesis of autism spectrum disorder with abnormal chromatin remodeling  [in Japanese]

    Katayama Yuta

    … Exome sequencing analysis has identified many de novo mutations in a variety of genes in individuals with ASD, with CHD8 being most frequently affected. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-S27-2, 2021

    J-STAGE 

  • Reduced white adipose tissue amount in mice lacking mitochondrial protein p13  [in Japanese]

    Satomi Hara , Ueno Hiroki , Noguchi Masafumi , Matsuo Wakana , Hashimoto Hitoshi , Shintani Norihito

    … Histological analysis revealed a marked difference in WAT rather than heart and brown adipose tissue (BAT) of p13-/- mice, in which significant decreases in adipocyte and lipid droplet size were observed. … Quantitative mRNA expression analysis showed p13-/- WAT exhibited decreasing tendencies of a mature adipocyte marker aP2, and of adipogenesis-related transcription factor C/EBPs. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-P2-LB49, 2021

    J-STAGE 

  • Analysis of the molecular mechanism of compensatory hypertrophy-associated enhancement of salivary secretion using the intravital Ca<sup>2+ </sup>imaging and gene analysis.  [in Japanese]

    Nezu Akihiro , Takahashi Shigeru , Morita Takao , Tanimura Akihiko

    … To clarify the molecular mechanism of this compensatory hyperfunction of SMG, we examined effects of the MED ligation on changes in salivary secretion, Ca<sup>2+ </sup>response and gene expression in SMG. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-P2-18, 2021

    J-STAGE 

  • The development of miR-96-5p gene knock out technique by CRISPR/Cas9 system  [in Japanese]

    Matsumura Nobuko , Kinoshita Chisato , Nakaki Toshio , Aoyama Koji

    … In order to investigate unknown features of miR-96-5p in various cell types in vitro, we developed the miR-96-5p gene knockout (KO) system by using CRISPR/Cas9. … In the analysis of DNA sequence, the mutations were found in the expected site in miR-96 gene after the plasmid was electroporated in HEK293 cells. … These results show that PX459-miR-96 KO plasmid successfully knockout the miR-96 gene in a human cell line.</p> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-P1-37, 2021

    J-STAGE 

  • Left-right asymmetry in chronic stress-induced neuroinflammation  [in Japanese]

    Matsushita Kazutoshi , Taniguchi Masayuki , Furuyashiki Tomoyuki

    … Here we found that chronic social stress induced left-right asymmetric changes of gene expression in mPFC microglia using laser microdissection microscopy followed by RNA sequencing. … Gene ontology analysis revealed that the genes upregulated in microglia of the right, but not left, mPFC enriched immune response-related genes. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-P1-18, 2021

    J-STAGE 

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