Search Results 1-20 of 247

  • APOBEC3B reporter myeloma cell lines identify DNA damage response pathways leading to APOBEC3B expression

    Yamazaki Hiroyuki , Shirakawa Kotaro , Matsumoto Tadahiko , Kazuma Yasuhiro , Matsui Hiroyuki , Horisawa Yoshihito , Stanford Emani , Sarca Anamaria Daniela , Shirakawa Ryutaro , Shindo Keisuke , Takaori-Kondo Akifumi

    … Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like (APOBEC) DNA cytosine deaminase 3B (A3B) is a DNA editing enzyme which induces genomic DNA mutations in multiple myeloma and in various other cancers. … These results suggest that DNA damage triggers A3B expression through ATM, ATR and DNA-PK signaling. …

    PLOS ONE 15(1), 2020-01-08

    IR 

  • Biomarkers in rheumatoid arthritis management  [in Japanese]

    Ohmura Koichiro

    <p> バイオマーカーを測定する意義は,より正確な診断,正確な活動性評価,予後予測評価,疾患分類などがあるが,血清中の自己抗体やサイトカイン,細胞表面マーカー,ゲノムデータ,遺伝子発現,miRNA,メタボロームなどが用いられうる.抗CCP抗体はRAの特異的診断マーカーであるが,膠原病ではしばしば陽性になるため注意が必要である.また,RFと組み合わせて4群に分類することで骨破壊の予後予測 …

    Clinical Rheumatology and Related Research 32(1), 68-74, 2020

    J-STAGE  Ichushi Web 

  • Modification of Mannosylerythritol Lipids (MELs) Producing Yeast by Genetic Engineering  [in Japanese]

    SAIKA Azusa

    <p>マンノシルエリスリトールリピッド(MEL)は糖型バイオサーファクタントのひとつであり,そのユニークで多彩な機能から,界面活性剤としての利用だけでなく,環境適合型の機能性バイオ化学品として注目を集めている。現在,MELは化粧品・日用品分野で実用化されているが,今後のさらなる用途拡大,また医薬,農業,食品等の新規分野への参入には,生産性の向上と構造・機能の拡充が大きな課題となっている …

    Oleoscience 20(5), 187-193, 2020

    J-STAGE  Ichushi Web 

  • H4K20 monomethylation inhibition causes loss of genomic integrity in mouse preimplantation embryos

    SHIKATA Daiki , YAMAMOTO Takuto , HONDA Shinnosuke , IKEDA Shuntaro , MINAMI Naojiro

    … <p>Maintaining genomic integrity in mammalian early embryos, which are deficient in DNA damage repair, is critical for normal preimplantation and subsequent development. … Abnormalities in DNA damage repair in preimplantation embryos can cause not only developmental arrest, but also diseases such as congenital disorders and cancers. … Histone H4 lysine 20 monomethylation (H4K20me1) is involved in DNA damage repair and regulation of gene expression. …

    Journal of Reproduction and Development, 2020

    J-STAGE 

  • The mechanism of anticancer drug-induced DNA damage response and repair in cardiomyocytes  [in Japanese]

    Hosoda Hiroshi

    <p>生命の遺伝情報を担うゲノムDNAは外来内因の物理的化学的因子によって絶え間なく損傷を受けており,細胞はDNA損傷応答とその修復機構を発達させDNAの安定性を維持している.心臓の虚血性変化や圧過負荷に伴う心不全発症の増悪に心筋細胞のDNA損傷応答・修復機能の障害が指摘されている.がん治療によって引き起こされる心毒性は,がんの予後とは関係なく患者の心血管発症リスクを高めQOLに強い影 …

    Folia Pharmacologica Japonica 155(3), 175-178, 2020

    J-STAGE  Ichushi Web 

  • The piRNA pathway in <i>Drosophila</i> ovarian germ and somatic cells

    SATO Kaoru , SIOMI Mikiko C.

    … Deterioration of the system causes DNA damage, leading to severe defects in gametogenesis and infertility. … ovaries show that piRNAs originate from specific genomic loci, termed piRNA clusters, and that in piRNA biogenesis, cluster transcripts are processed into mature piRNAs via three distinct pathways: initiator or responder for ping-pong piRNAs and trailing for phased piRNAs. …

    Proceedings of the Japan Academy, Series B 96(1), 32-42, 2020

    J-STAGE 

  • Processing of a single ribonucleotide embedded into DNA by human nucleotide excision repair and DNA polymerase η

    Sassa Akira , Tada Haruto , Takeishi Ayuna , Harada Kaho , Suzuki Megumi , Tsuda Masataka , Sasanuma Hiroyuki , Takeda Shunichi , Sugasawa Kaoru , Yasui Manabu , Honma Masamitsu , Ura Kiyoe

    … DNA polymerases often incorporate non-canonical nucleotide, i.e., ribonucleoside triphosphates into the genomic DNA. … Collectively, our results suggest that base oxidation of the embedded ribonucleotide enables processing of the ribonucleotide via alternative DNA repair and damage tolerance pathways. …

    Scientific Reports (9), 13910, 2019-09-26

    IR 

  • A Novel Diagnostic Method for Thyroid Follicular Tumors Based on Immunofluorescence Analysis of p53-Binding Protein 1 Expression: Detection of Genomic Instability

    Otsubo Ryota , Matsuda Katsuya , Mussazhanova Zhanna , Sato Ayako , Matsumoto Megumi , Yano Hiroshi , Oikawa Masahiro , Kondo Hisayoshi , Ito Masahiro , Miyauchi Akira , Hirokawa Mitsuyoshi , Nagayasu Takeshi , Nakashima Masahiro

    … 53BP1 belongs to a class of DNA damage response molecules that rapidly localize to the site of DNA double-strand breaks,forming nuclear foci (NF). … Furthermore, the study clarified the relationship between 53BP1 NF and copy number aberrations (CNAs) based on array comparative genomic hybridization, a hallmark of genomic instability (GIN). …

    Thyroid 29(5), 657-665, 2019-05-13

    IR 

  • Nucleolar protein nucleolin functions in replication stress–induced DNA damage responses

    Kawamura Kasumi , Qi Fei , Meng Qingmei , Hayashi Ikue , Kobayashi Junya

    … The nucleolus contains multiple copies of ribosomal (r)DNA, which indicate sites of frequent replication stress and suggest the existence of a mechanism to prevent replication stress–related rDNA instability and the possibility that such a mechanism contributes to the whole genomic stability against replication stress. …

    Journal of Radiation Research 60(3), 281-288, 2019-05

    IR  Ichushi Web 

  • PDIP38/PolDIP2 controls the DNA damage tolerance pathways by increasing the relative usage of translesion DNA synthesis over template switching

    Tsuda Masataka , Ogawa Saki , Ooka Masato , Kobayashi Kaori , Hirota Kouji , Wakasugi Mitsuo , Matsunaga Tsukasa , Sakuma Tetsushi , Yamamoto Takashi , Chikuma Shunsuke , Sasanuma Hiroyuki , Debatisse Michelle , Doherty Aidan J. , Fuchs Robert P. , Takeda Shunichi

    … Stalled replication forks are restored by the DNA damage tolerance (DDT) pathways, error-prone translesion DNA synthesis (TLS) to cope with excessive DNA damage, and error-free template switching (TS) by homologous DNA recombination. … We measured the relative usage of TLS and TS in TK6 cells at a chemically synthesized UV damage (CPD) integrated into genomic DNA. …

    PLOS ONE 14(3), 2019-03-06

    IR 

  • Mechanism and regulation of DNA damage recognition in nucleotide excision repair

    Kusakabe Masayuki , Onishi Yuki , Tada Haruto , Kurihara Fumika , Kusao Kanako , Furukawa Mari , Iwai Shigenori , Yokoi Masayuki , Sakai Wataru , Sugasawa Kaoru

    … In mammalian global genomic NER, the XPC protein complex initiates the repair reaction by recognizing sites of DNA damage, and this depends on detection of disrupted/destabilized base pairs within the DNA duplex. … Such multi-step strategy for damage recognition would contribute to achieve both versatility and accuracy of the NER system at substantially high levels. …

    Genes and Environment (41), 2, 2019-01-25

    IR 

  • Update on paraganglioma and pheochromocytoma based on WHO tumour classification 2017  [in Japanese]

    Kimura Noriko , Shiga Kiyoto , Kaneko Ken-ich , Takekoshi Kazuhiro

    褐色細胞腫(PCC)・パラガングリオーマ(PGL)はいずれも神経堤由来の腫瘍で,PCCは副腎髄質に,PGLは副腎外に生ずるものを呼ぶ。さらに,PGLは頭頸部に生ずる副交感神経性PGL(Head & Neck PGL:HNPGL)と,胸腹部・骨盤内に生ずる交感神経性PGLに分けられる。HNPGLは頸動脈小体腫瘍(CBT)60%,中耳PGL 30%,迷走神経PGL 10%である。また,家族性30%,両 …

    Toukeibu Gan 45(4), 350-353, 2019

    J-STAGE  Ichushi Web 

  • The role of DNA methylation in anticancer drug-induced DNA damage repair system in cardiomyocytes.  [in Japanese]

    Hosoda Hiroshi

    … <p>Genomic DNA, which contains all of the genetic information, is damaged by a variety of endogenous and environmental factors such as genotoxic chemicals, ionizing radiation and UV light. … Consequently, the DNA repair process is constantly active as it responds to damage in the DNA structure. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 3-S22-3, 2019

    J-STAGE 

  • DNA damage induced by ultrasound and its cellular response  [in Japanese]

    FURUSAWA Yukihiro , KUDO Nobuki , KONDO Takashi

    <p>超音波の生物作用は熱的作用と非熱的作用に分類され,超音波によるDNA損傷は主として非熱的作用によるものと考えられている.胎児診断の安全性の観点から多くの<I>in vitro</I>研究がなされてきた.DNAに生じる変化の検出技術の進歩とも相まって,水溶液内のDNAへの影響および細胞内DNAへの影響とその作用機序が急速に解明されつつある.一方でDNA損傷は …

    Choonpa Igaku 47(1), 3-13, 2019

    J-STAGE 

  • Epidemiology of Classical Swine Fever in Pigs and Wild Animals  [in Japanese]

    YAMAMOTO Takehisa

    … Due to its very high infectivity and lack of specific clinical feature, and by damaging the chance of international trades, it poses a severe damage to pig industry. … A genomic analysis of the viral strain isolated from the infected farm revealed that the virus is distinct from that in the previous outbreak in Japan and similar to the strains found in the recent outbreaks in east Asian countries.</p> …

    Journal of Veterinary Epidemiology 23(1), 1-3, 2019

    J-STAGE  Ichushi Web 

  • Development of molecular marker and introgression of <i>Bph3</i> into elite rice cultivars by marker-assisted selection

    Qing Dongjin , Chen Rentian , Chen Weiwei , Huang Fengkuan , Deng Guofu , Dai Gaoxing , Zhou Weiyong , Huang Suosheng , Liang Haifu , Gao Lijun , Gao Ju , Huang Juan , Zhou Meng

    … Identification of new BPH resistance genes and their transfer into modern rice cultivars are effective breeding approaches to reduce the damage caused by BPH. … In this study, we mapped a BPH resistance gene to a 50-kb genomic interval between two InDel markers 4M03980 and 4M04041 on the short arm of chromosome 4 in <i>indica</i> … and using them for molecular breeding will facilitate the creation of BPH-resistance rice cultivars to reduce damage caused by BPH.</p> …

    Breeding Science 69(1), 40-46, 2019

    J-STAGE 

  • Genomic landscape of oxidative DNA damage and repair reveals regioselective protection from mutagenesis

    Anna R. Poetsch , Simon J. Boulton , Nicholas M. Luscombe

    … BACKGROUND: DNA is subject to constant chemical modification and damage, which eventually results in variable mutation rates throughout the genome. … Although detailed molecular mechanisms of DNA damage and repair are well understood, damage impact and execution of repair across a genome remain poorly defined. …

    Genome Biology 19(1), 215, 2018-12-07

    IR 

  • Expression pattern of p53-binding protein 1 as a new molecular indicator of genomic instability in bladder urothelial carcinoma

    Matsuda Katsuya , Kawasaki Tatsuhiko , Akazawa Yuko , Hasegawa Yuhmi , Kondo Hisayoshi , Suzuki Keiji , Iseki Masachika , Nakashima Masahiro

    … Our previous study suggested that co-expression of Ki-67 and p53-binding protein 1 (53BP1) could provide an indicator of an abnormal DNA damage response (DDR) pathway. … This study identified clear differences in the 53BP1 expression patterns in urothelial carcinogenesis, and their close association with genomic instability. …

    Scientific Reports 8(1), 15477, 2018-10-19

    IR 

  • Potential relationship between the biological effects of low-dose irradiation and mitochondrial ROS production

    Kawamura Kasumi , Qi Fei , Kobayashi Junya

    … Exposure to ionizing radiation (IR) induces various types of DNA damage, of which DNA double-strand breaks are the most severe, leading to genomic instability, tumorigenesis, and cell death. … Hence, cells have developed DNA damage responses and repair mechanisms. … Upon exposure to low-dose irradiation, the IR-induced biological effects mediated by ROS were relatively more significant than those mediated by DNA damage. …

    Journal of Radiation Research 59(suppl_2), ii91-ii97, 2018-04

    IR 

  • Hypersensitivity of mouse embryonic fibroblast cells defective for DNA polymerases η, ι and κ to various genotoxic compounds: Its potential for application in chemical genotoxic screening

    Akagi Jun-ichi , Yokoi Masayuki , Cho Young-Man , Toyoda Takeshi , Ohmori Haruo , Hanaoka Fumio , Ogawa Kumiko

    … Genotoxic agents cause modifications of genomic DNA, such as alkylation, oxidation, bulky adduct formation, and strand breaks, which potentially induce mutations and changes to the structure or number of genes. … Additionally, the two markers for DNA damage, ubiquitylated proliferating cell nuclear antigen and γ-H2AX after MMS treatment, were accumulated in TKO cells more greatly than in WT cells. …

    DNA Repair (61), 76-85, 2018-01

    IR 

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