Search Results 1-20 of 304

  • Sperm Motility Regulation in Male and Female Bird Genital Tracts

    Matsuzaki Mei , Sasanami Tomohiro

    … <p>Sperm drastically change their flagellar movement in response to the surrounding physical and chemical environment. … however, they gain the competence to initiate motility during passage through the male reproductive tract. … Unlike mammals, ejaculated sperm in birds are stored in specialized tubular invaginations referred to as sperm storage tubules (SSTs), located between the vagina and uterus, before fertilization. …

    The Journal of Poultry Science, 2021

    J-STAGE 

  • Evaluation of <i>in vitro</i> biofilm elimination of <i>Enterococcus faecalis</i> using a continuous ultrasonic irrigation device

    Galván-Pacheco Jennifer , Vitales-Noyola Marlen , González-Amaro Ana M. , Bujanda-Wong Heriberto , Aragón-Piña Antonio , Méndez-González Verónica , Pozos-Guillén Amaury

    … The HBW Ultrasonic Ring promoted a higher reduction in bacterial load relative to conventional irrigation (<i>P</i> … These results suggest the HBW Ultrasonic Ring is a promising alternative modality for simultaneous instrumentation and irrigation during root canal treatment, achieving an appropriate level of bacterial reduction and allowing the passage of the irrigating solution throughout the entire working length.</p> …

    Journal of Oral Science 62(4), 415-419, 2020

    J-STAGE 

  • Measurement of axonal conduction velocity in in vitro peripheral neurons using CMOS-MEA  [in Japanese]

    TAKAHASHI Sayuri , KATO Yuri , ODAWARA Aoi , MATSUDA Naoki , TAURA Tadayuki , OIKE Yusuke , SUZUKI Ikuro

    in vitroで軸索伝導速度を指標とした薬の評価ができれば、毒性評価の新規プラットフォームとなる可能性があるが、軸索伝導速度計測には、高時空間分解能計測が必要である。 … 本計測法は、軸索内伝導速度を指標としたin vitroの薬効および毒性評価法として有効であると考えられる。 …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-235, 2020

    J-STAGE 

  • Pharmacology of Baloxavir (Xofluza<sup>®</sup>); a First-in-Class Cap-dependent Endonuclease Inhibitor for Treatment of Influenza  [in Japanese]

    Takao Shishido

    … Baloxavir was approved for treatment of uncomplicated influenza A and B virus infections in 2018 in Japan (for those weighing &gt;10 kg) and the United States (for those aged 12 years and older) </p><p>Key nonclinical characteristics of baloxavir include broad spectrum activity against various types and subtypes of influenza virus strains <i>in vitro</i> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-LBS-30, 2020

    J-STAGE 

  • Leukocyte-mimetic liposomes possessing leukocyte membrane proteins pass through inflamed endothelial cell layer by regulating intercellular junctions

    Fukuta Tatsuya , Yoshimi Shintaro , Tanaka Tamotsu , Kogure Kentaro

    … As leukocytes can pass through the inflamed endothelium via utilizing membrane protein functions, we hypothesized that incorporating leukocyte membrane proteins onto liposomal membranes may impart leukocyte-mimicking functions to liposomes, allowing for their adherence to and active passage through the inflamed endothelium. … Herein, we developed leukocyte-mimetic liposomes (LM-Lipo) by leukocyte membrane protein transfer and evaluated their function in vitro. …

    International Journal of Pharmaceutics (563), 314-323, 2019-04-10

    IR 

  • A robust culture method for maintaining tumorigenic cancer stem cells in the hepatocellular carcinoma cell line Li‐7

    山田 武史 , 長谷川 直之 , 兵頭 一之介 , Yukako Sato , Takeshi YAMADA , Takashi Hiroyama , Kazuhiro Sudo , Naoyuki HASEGAWA , Ichinosuke HYODO , Yukio Nakamura

    … To ensure consistent and reproducible results in experiments using Li‐7 cells, it is important that the CSC population is maintained stably regardless of culture duration and passage. …

    Cancer science 110(5), 1644-1652, 2019-02

    IR  Ichushi Web 

  • The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture

    Shioda Setsuko , Kasai Fumio , Ozawa Midori , Hirayama Noriko , Satoh Motonobu , Kameoka Yousuke , Watanabe Ken , Shimizu Norio , Tang Huamin , Mori Yasuko , Kohara Arihiro

    … Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. … Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. …

    Cytotechnology 70(1), 141-152, 2018-02

    IR 

  • Cell culture-induced variation of the HepG2 cell line proteome

    Ellero Andrea A , Cromarty Andrew D , Hurrell Tracey

    … <p>Background: Standardizing in vitro pre-clinical hepatotoxicity is confounded by diverse cellular origins which often lack representative hepatocellular function. … HepG2 cells, with a stable phenotype in early passage, have been extensively used in hepatotoxicity screening despite an apparent poor metabolic competence and "foetal-like" …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO4-9-26, 2018

    J-STAGE 

  • The effects of histidine-rich glycoprotein on neutrophil-like differentiated cell lines

    Yoshii Yukinori , Wake Hidenori , Teshigawara Kiyoshi , Liu Keyue , Wang Dengli , Takahashi Yohei , Nishibori Masahiro

    … <p>Background: Excess immunothrombus formation induced by neutrophil adhesion on vascular wall, neutrophil extracellular traps (NETs) release and ROS production causes the multiple organ failure (MOF) in septic condition, leading to high mortality. … These results suggested that HRG may be one of the most useful strategy for the treatment of septic patients, however, the effects of HRG on human purified neutrophil are not understood in detail. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO2-5-14, 2018

    J-STAGE 

  • The synergistic antitumor effect of combined Trastuzumab and gamma interferon therapy to drug resistant Human Epidermal Growth Factor Receptor 2 (HER2) positive breast cancer cells

    Gocho Toshihiko , Tsuchiya Hiromichi , Kamijyo Shotaro , Yamazaki Yoshitaka , Akita Yui , Ao Yoko , Sasaki Akiko , Kiuchi Yuji

    … <p>We reported that Trastuzumab and gamma interferon combined therapy showed higher synergistic antitumor effect than usual therapy in vitro and in vivo mouse model before.</p><p> …  In this study we evaluated whether Trastuzumab and gamma interferon combined therapy shows good synergy effect to drug resistant HER2 positive human breast cancer cells or not. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO1-8-19, 2018

    J-STAGE 

  • [MEASURE] 5. <i>In vitro</i> detection of transformed cells - Preliminary studies of cellular immortality testing -  [in Japanese]

    WATANABE Sono , KONO Ken , TAKAHASHI Chihiro , HATA Shinko , SUZUKI Mutsumi , SATO Yoji , SHIRAISHI Norihiko

    … <p>[Introduction] The differentiated cells derived from pluripotent stem cells (PSCs) have potentials to transform to malignant cells and thus, the establishment of validated assays to detect the malignant transformed cells in cell therapy products (CTPs) is of paramount importance for minimizing the risk of PSCs-based tumorigenicity. …

    Annual Meeting of the Japanese Society of Toxicology 45.1(0), P-217, 2018

    J-STAGE 

  • Altered properties of feline adipose-derived mesenchymal stem cells during continuous <i>in vitro</i> cultivation

    LEE Bo-Yeon , LI Qiang , SONG Woo-Jin , CHAE Hyung-Kyu , KWEON Kyeong , AHN Jin-Ok , YOUN Hwa-Young

    … <p>Cytotherapy with mesenchymal stem cells (MSCs) has been studied in many species, and often requires <i>in vitro</i> … This study assessed the changes in the characteristics of feline adipose tissue-derived (fAT)-MSCs during <i>in vitro</i> … The cell proliferation rate decreased significantly at passage 5 (P5). …

    Journal of Veterinary Medical Science 80(6), 930-938, 2018

    J-STAGE  Ichushi Web 

  • Characterization of canine coronavirus spread among domestic dogs in Vietnam

    van NGUYEN Dung , TERADA Yukata , MINAMI Shohei , YONEMITSU Kenzo , NAGATA Nao , LE Thanh Dinh Ha , KUWATA Ryusei , SHIMODA Hiroshi , MAEDA Ken

    … <p>Canine coronavirus (CCoV) is an important pathogen that causes enteritis in dogs, but there is no information on CCoV infection in Vietnam. … The results showed that antibody against CCoV-II was present in 87 dogs (43.3%). … Furthemore, we succeeded in isolating CCoV/dog/HCM47/2015, the isolate was plaque-purified three times, and 3'-terminal one-third of the genome was analyzed. …

    Journal of Veterinary Medical Science 79(2), 343-349, 2017

    J-STAGE  Ichushi Web 

  • The detection of milk fat in some commercial milk and dairy products on Lipase hydrolysis(3)Effect of detection of Lipase hydrolysis by intensifying emulsification  [in Japanese]

    ONJI Masashi , OKA Terumi

    … To evaluate in vitro digestion of milk fat in some commercial dairy products as butter and fat spreader as margarine, the distinctive digestion on lipase hydrolysis by intensifying emulsification, was investigated in 4 different conditions at 40℃ for 0, 15, 30 and 60 minutes, respectively. …

    久留米信愛女学院短期大学研究紀要 (39), 27-32, 2016-07

    NDL Digital Collections 

  • An <i>In Vitro</i> Contraction Model in Mouse Primary Cultured Myotubes Using Satellite Cells Originated from the EDL and Soleus Muscles

    MANABE YASUKO , OGINO SHINYA , ITO MIYUKI , FURUICHI YASURO , TAKAGI MAYUMI , YAMADA MIO , GOTO-INOUE NAOKO , ONO YUSUKE , FUJII NOBUHARU L.

    … <p><b><i>Purpose: </i></b>Skeletal muscle cell lines such as mouse C2C12 cells and rat L6 cells often show abnormal characteristics because of repeated-passage cultures and artificial culture conditions. … Primary myotubes are considered to retain their <i>in vivo</i> …

    Juntendo Medical Journal 62(Suppl.1), 143-143, 2016

    J-STAGE 

  • Impact of the Maraviroc-Resistant Mutation M434I in the C4 Region of HIV-1 gp120 on Sensitivity to Antibody-Mediated Neutralization

    Boonchawalit Samatchaya , Harada Shigeyoshi , Shirai Noriko , Gatanaga Hiroyuki , Oka Shinichi , Matsushita Shuzo , Yoshimura Kazuhisa

    … We previously reported that a maraviroc (MVC)-resistant human immunodeficiency virus type 1variant, generated using in vitro selection, exhibited high sensitivity to several neutralizing monoclonal antibodies (NMAbs) and autologous plasma IgGs. … The MVC-resistant variant acquired 4 sequential mutations in gp120: T297I, M434I, V200I, and K305R. …

    Japanese Journal of Infectious Diseases 69(3), 236-243, 2016

    J-STAGE 

  • Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells

    RUNGSIWIWUT Ruttachuk , NUMCHAISRIKA Pranee , AHNONKITPANIT Vichuda , VIRUTAMASEN Pramuan , PRUKSANANONDA Kamthorn

    In the present study, a total of 20 tripronuclear zygotes were cultured; … Although the cell line displayed an abnormal chromosome number, it was found the cell line has been shown to be pluripotent with the ability to differentiate into 3 embryonic germ layers both <i>in vitro</i> … and <i>in vivo</i>. …

    Journal of Reproduction and Development 62(2), 167-176, 2016

    J-STAGE  Ichushi Web 

  • Ultrastructural comparison of porcine putative embryonic stem cells derived by <i>in vitro</i> fertilization and somatic cell nuclear transfer

    YOO Hyunju , LEE Eunsong , KIM Hyunggee , KIM Gonhyung , HYUN Sang-Hwan , KIM Eunhye , HWANG Seon-Ung , YOON Junchul David , JEON Yubyeol , PARK Kyu-Mi , KIM Kyu-Jun , JIN Minghui , LEE Chang-Kyu

    … The aim of this study was to compare the features of organelles in <i>in vitro</i> … Also, the features of organelles in high-passage IVF-pESCs were compared with those in low-passage cells. …

    Journal of Reproduction and Development 62(2), 177-185, 2016

    J-STAGE  Ichushi Web 

  • The detection of milk fat in some commercial milk and dairy products on Lipase hydrolysis(2)  [in Japanese]

    ONJI Masashi , OKA Terumi

    … To evaluate in vitro digestion of milk fat in some commercial dairy products as butter and fat spreader as margarine, the distinctive digestion on lipase hydrolysis was investigated in 4 different conditions at 40 ℃ for 0, 15, 30 and 60 minutes, respectively. … The digestion comparatively was differentiated concentration of substances, passage ofreactive time, and content of percentage on oils and fats. …

    久留米信愛女学院短期大学研究紀要 (38), 35-39, 2015-07

    NDL Digital Collections 

  • Morphological study of boar sperm during their passage through the female genital tract

    GARCÍA-VÁZQUEZ Francisco Alberto , HERNÁNDEZ-CARAVACA Iván , MATÁS Carmen , SORIANO-ÚBEDA Cristina , ABRIL-SÁNCHEZ Silvia , IZQUIERDO-RICO María José

    … Once deposited in the female tract, sperm face a series of challenges that must be overcome to ensure the presence of an adequate normal sperm population close to the site of fertilization. …

    Journal of Reproduction and Development 61(5), 407-413, 2015

    J-STAGE  Ichushi Web 

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